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Application of improved single blastomere fixation technique in preimplantation genetic diagnosis.
Cytotechnology. 2020 Apr; 72(2):217-226.C

Abstract

To modify a fixation method improving the intensity and clarity of the single blastomeric signal detection by fluorescence in situ hybridization (FISH) in preimplantation genetic diagnosis. 333 cycles of assisted reproduction with preimplantation genetic diagnosis FISH (PGD-FISH) performed in our hospital were analyzed and a total of 3452 single blastomeres were obtained. For the conventional fixation method, the blastomeres were kept in 0.1% sodium citrate with 0.2 mg/ml bovine serum albumin (BSA) for 2-5 min. FISH was performed and the internal relationship between embryo quality and fixed rate, signal detection rate, and signal determination rate was explored. With the modified method, 91.54% of blastomeres were fixed, while 88.30% were fixed with the conventional method. The signal detection rate was significantly increased for the modified group than for the conventional group (compared 98.53% with 94.78%, P < 0.001). Especially, the signal determination rate also showed a significant difference between the two methods (compared 90.51% with 74.17%, P < 0.001). After the development of the fixation method, the fixation efficiency and the signal determination rate were greatly improved, providing more definite diagnosis for the patient. It will hopefully allow more assisted reproduction programs to offer their patients preimplantation genetic diagnosis with FISH.

Authors+Show Affiliations

Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, Jinan, 250021, Shandong, China.Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, Jinan, 250021, Shandong, China.Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, Jinan, 250021, Shandong, China.Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, Jinan, 250021, Shandong, China.Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, Jinan, 250021, Shandong, China.Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, Jinan, 250021, Shandong, China. wukeliang_527@163.com.Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, Jinan, 250021, Shandong, China. aijunhao@sdu.edu.cn. Department of Histology and Embryology, Shandong University School of Medicine, No. 44, Wenhua Xi Road, Jinan, 250012, Shandong, China. aijunhao@sdu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32232612

Citation

Yu, Guanling, et al. "Application of Improved Single Blastomere Fixation Technique in Preimplantation Genetic Diagnosis." Cytotechnology, vol. 72, no. 2, 2020, pp. 217-226.
Yu G, Ma S, Zhu Y, et al. Application of improved single blastomere fixation technique in preimplantation genetic diagnosis. Cytotechnology. 2020;72(2):217-226.
Yu, G., Ma, S., Zhu, Y., Liu, Y., Zhang, H., Wu, K., & Hao, A. (2020). Application of improved single blastomere fixation technique in preimplantation genetic diagnosis. Cytotechnology, 72(2), 217-226. https://doi.org/10.1007/s10616-020-00371-1
Yu G, et al. Application of Improved Single Blastomere Fixation Technique in Preimplantation Genetic Diagnosis. Cytotechnology. 2020;72(2):217-226. PubMed PMID: 32232612.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of improved single blastomere fixation technique in preimplantation genetic diagnosis. AU - Yu,Guanling, AU - Ma,Shuiying, AU - Zhu,Yueting, AU - Liu,Yujin, AU - Zhang,Haozhen, AU - Wu,Keliang, AU - Hao,Aijun, Y1 - 2020/03/31/ PY - 2019/07/29/received PY - 2020/01/09/accepted PY - 2021/04/01/pmc-release PY - 2020/4/2/pubmed PY - 2020/4/2/medline PY - 2020/4/2/entrez KW - Blastomere KW - Diagnosis KW - FISH KW - Fixation KW - Preimplantation SP - 217 EP - 226 JF - Cytotechnology JO - Cytotechnology VL - 72 IS - 2 N2 - To modify a fixation method improving the intensity and clarity of the single blastomeric signal detection by fluorescence in situ hybridization (FISH) in preimplantation genetic diagnosis. 333 cycles of assisted reproduction with preimplantation genetic diagnosis FISH (PGD-FISH) performed in our hospital were analyzed and a total of 3452 single blastomeres were obtained. For the conventional fixation method, the blastomeres were kept in 0.1% sodium citrate with 0.2 mg/ml bovine serum albumin (BSA) for 2-5 min. FISH was performed and the internal relationship between embryo quality and fixed rate, signal detection rate, and signal determination rate was explored. With the modified method, 91.54% of blastomeres were fixed, while 88.30% were fixed with the conventional method. The signal detection rate was significantly increased for the modified group than for the conventional group (compared 98.53% with 94.78%, P < 0.001). Especially, the signal determination rate also showed a significant difference between the two methods (compared 90.51% with 74.17%, P < 0.001). After the development of the fixation method, the fixation efficiency and the signal determination rate were greatly improved, providing more definite diagnosis for the patient. It will hopefully allow more assisted reproduction programs to offer their patients preimplantation genetic diagnosis with FISH. SN - 0920-9069 UR - https://www.unboundmedicine.com/medline/citation/32232612/Application_of_improved_single_blastomere_fixation_technique_in_preimplantation_genetic_diagnosis L2 - https://dx.doi.org/10.1007/s10616-020-00371-1 DB - PRIME DP - Unbound Medicine ER -
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