Tags

Type your tag names separated by a space and hit enter

Long Noncoding RNA POU3F3 and α-Synuclein in Plasma L1CAM Exosomes Combined with β-Glucocerebrosidase Activity: Potential Predictors of Parkinson's Disease.
Neurotherapeutics. 2020 07; 17(3):1104-1119.N

Abstract

Long noncoding RNAs (lncRNAs) are implicated in the autophagic-lysosomal pathway (ALP) and are closely linked to Parkinson's disease (PD) pathology. β-Glucocerebrosidase (GCase) has also been reported to be correlated with α-synuclein (α-syn) proteostasis. However, lncRNAs and α-syn in neural-derived L1CAM exosomes and GCase activity in the plasma of PD patients have not been studied. This study used an ultrasensitive methodology, fluorescence nanoparticle tracking analysis (NTA), to measure plasma L1CAM exosomes and Quanterix Simoa to measure α-syn concentrations in L1CAM exosomes. Eighty-five healthy controls and 93 PD patients were enrolled, and several scales were used to rate the severity of PD. Receiver operating characteristic (ROC) curves were applied to map the diagnostic accuracy of categorizing PD patients and healthy subjects. We found increased Linc-POU3F3 and α-syn concentrations in L1CAM exosomes and decreased GCase activity in PD patients compared with controls. The three biomarkers displayed obvious differences among PD patients based on gender, H-Y stage, and UPDRS-III distribution. Interestingly, Linc-POU3F3 was significantly positively correlated with α-syn in L1CAM exosomes and inversely correlated with GCase activity in PD patients. Significant correlations were observed among L1CAM exosomal Linc-POU3F3 levels, GCase activity, and PD severity, including motor/cognitive dysfunction. Additionally, the combination of Linc-POU3F3 and α-syn in L1CAM exosomes and GCase activity could discriminate PD patients from controls. These results suggest that L1CAM exosomal Linc-POU3F3, L1CAM exosomal α-syn, and GCase activity may shed light on the mechanism underlying the autophagic-lysosomal system in the pathogenesis of PD and could be used to assess the severity of PD.

Authors+Show Affiliations

Department of Neurology and Stroke Center, The First Affiliated Hospital, Jinan University, Guangzhou, 510630, Guangdong, People's Republic of China. Clinical Neuroscience Institute of Jinan University, Guangzhou, 510630, Guangdong, People's Republic of China.Key Laboratory of Assisted Circulation, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong, People's Republic of China. NHC Key Laboratory of Assisted Circulation, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong, People's Republic of China.Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, Guangdong, People's Republic of China.Department of Neurology, University of Pittsburgh, Pittsburgh, Pennsylvania, 15213, USA.Department of Medicine, Lincoln Medical Center, Bronx, New York, 10451, USA.Department of Neurology and Stroke Center, The First Affiliated Hospital, Jinan University, Guangzhou, 510630, Guangdong, People's Republic of China. tlil@jnu.edu.cn. Clinical Neuroscience Institute of Jinan University, Guangzhou, 510630, Guangdong, People's Republic of China. tlil@jnu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32236821

Citation

Zou, Jing, et al. "Long Noncoding RNA POU3F3 and α-Synuclein in Plasma L1CAM Exosomes Combined With β-Glucocerebrosidase Activity: Potential Predictors of Parkinson's Disease." Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics, vol. 17, no. 3, 2020, pp. 1104-1119.
Zou J, Guo Y, Wei L, et al. Long Noncoding RNA POU3F3 and α-Synuclein in Plasma L1CAM Exosomes Combined with β-Glucocerebrosidase Activity: Potential Predictors of Parkinson's Disease. Neurotherapeutics. 2020;17(3):1104-1119.
Zou, J., Guo, Y., Wei, L., Yu, F., Yu, B., & Xu, A. (2020). Long Noncoding RNA POU3F3 and α-Synuclein in Plasma L1CAM Exosomes Combined with β-Glucocerebrosidase Activity: Potential Predictors of Parkinson's Disease. Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics, 17(3), 1104-1119. https://doi.org/10.1007/s13311-020-00842-5
Zou J, et al. Long Noncoding RNA POU3F3 and α-Synuclein in Plasma L1CAM Exosomes Combined With β-Glucocerebrosidase Activity: Potential Predictors of Parkinson's Disease. Neurotherapeutics. 2020;17(3):1104-1119. PubMed PMID: 32236821.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long Noncoding RNA POU3F3 and α-Synuclein in Plasma L1CAM Exosomes Combined with β-Glucocerebrosidase Activity: Potential Predictors of Parkinson's Disease. AU - Zou,Jing, AU - Guo,Yue, AU - Wei,Lei, AU - Yu,Fang, AU - Yu,Bo, AU - Xu,Anding, PY - 2020/4/3/pubmed PY - 2021/9/2/medline PY - 2020/4/3/entrez KW - Autophagic-lysosomal pathway KW - L1CAM exosomes KW - Linc-POU3F3 KW - Parkinson’s disease KW - α-synuclein SP - 1104 EP - 1119 JF - Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics JO - Neurotherapeutics VL - 17 IS - 3 N2 - Long noncoding RNAs (lncRNAs) are implicated in the autophagic-lysosomal pathway (ALP) and are closely linked to Parkinson's disease (PD) pathology. β-Glucocerebrosidase (GCase) has also been reported to be correlated with α-synuclein (α-syn) proteostasis. However, lncRNAs and α-syn in neural-derived L1CAM exosomes and GCase activity in the plasma of PD patients have not been studied. This study used an ultrasensitive methodology, fluorescence nanoparticle tracking analysis (NTA), to measure plasma L1CAM exosomes and Quanterix Simoa to measure α-syn concentrations in L1CAM exosomes. Eighty-five healthy controls and 93 PD patients were enrolled, and several scales were used to rate the severity of PD. Receiver operating characteristic (ROC) curves were applied to map the diagnostic accuracy of categorizing PD patients and healthy subjects. We found increased Linc-POU3F3 and α-syn concentrations in L1CAM exosomes and decreased GCase activity in PD patients compared with controls. The three biomarkers displayed obvious differences among PD patients based on gender, H-Y stage, and UPDRS-III distribution. Interestingly, Linc-POU3F3 was significantly positively correlated with α-syn in L1CAM exosomes and inversely correlated with GCase activity in PD patients. Significant correlations were observed among L1CAM exosomal Linc-POU3F3 levels, GCase activity, and PD severity, including motor/cognitive dysfunction. Additionally, the combination of Linc-POU3F3 and α-syn in L1CAM exosomes and GCase activity could discriminate PD patients from controls. These results suggest that L1CAM exosomal Linc-POU3F3, L1CAM exosomal α-syn, and GCase activity may shed light on the mechanism underlying the autophagic-lysosomal system in the pathogenesis of PD and could be used to assess the severity of PD. SN - 1878-7479 UR - https://www.unboundmedicine.com/medline/citation/32236821/Long_Noncoding_RNA_POU3F3_and_α_Synuclein_in_Plasma_L1CAM_Exosomes_Combined_with_β_Glucocerebrosidase_Activity:_Potential_Predictors_of_Parkinson's_Disease_ L2 - https://dx.doi.org/10.1007/s13311-020-00842-5 DB - PRIME DP - Unbound Medicine ER -