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[Effect of lead pollution on anticoagulant effect of Whitmania pigra based on in vitro anticoagulation experiment].
Zhongguo Zhong Yao Za Zhi. 2019 Dec; 44(23):5114-5117.ZZ

Abstract

Leech has a good anticoagulant activity and is one of the raw materials for treatment of many cardiovascular and cerebrovascular diseases. This study was based on in vitro anticoagulant experiments( APTT and PT) to investigate the effects of lead contamination on the anticoagulant effect of leech. At present,the Hirudo circulating in the market are dominated by Whitmania pigra,therefore Wh. pigra were cultivated under a different lead pollution for 50 days. Then,the effects of Wh. pigra extract,extracting from different cultivating environment,on activated partial thrombin time( APTT) and prothrombin time( PT) were determined by automatic coagulation instrument. The results showed that the Wh. pigra extract significantly prolonged the APTT compared with the saline group.The APTT of the lead-high residual Wh. pigra was shorter than that of the blank Wh. pigra. The Wh. pigra extracts from different treatment groups had little effect on PT. The results showed that the lead residue in the Wh. pigra increased with the increase of lead in the cultured soil,the lead residual of the Pb-H group was( 10. 66±2. 79) mg·kg~(-1),which exceeded the lead limit specified in the 2015 edition of the Chinese Pharmacopoeia. The results indicated that growth environment pollution is one of the important factors causing excessive lead in Wh. pigra. Lead pollution will reduce the anticoagulant effect of Wh. pigra and affect its clinical efficacy.

Authors+Show Affiliations

School of Chinese Medicine,Beijing University of Chinese Medicine Beijing 102488,China.School of Chinese Medicine,Beijing University of Chinese Medicine Beijing 102488,China.School of Chinese Medicine,Beijing University of Chinese Medicine Beijing 102488,China.School of Chinese Medicine,Beijing University of Chinese Medicine Beijing 102488,China.School of Chinese Medicine,Beijing University of Chinese Medicine Beijing 102488,China.School of Chinese Medicine,Beijing University of Chinese Medicine Beijing 102488,China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

32237346

Citation

Luo, Xue-Mei, et al. "[Effect of Lead Pollution On Anticoagulant Effect of Whitmania Pigra Based On in Vitro Anticoagulation Experiment]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 44, no. 23, 2019, pp. 5114-5117.
Luo XM, Meng JQ, Chen XF, et al. [Effect of lead pollution on anticoagulant effect of Whitmania pigra based on in vitro anticoagulation experiment]. Zhongguo Zhong Yao Za Zhi. 2019;44(23):5114-5117.
Luo, X. M., Meng, J. Q., Chen, X. F., Xu, Q., Zhang, F., & Yang, Y. J. (2019). [Effect of lead pollution on anticoagulant effect of Whitmania pigra based on in vitro anticoagulation experiment]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 44(23), 5114-5117. https://doi.org/10.19540/j.cnki.cjcmm.20190920.101
Luo XM, et al. [Effect of Lead Pollution On Anticoagulant Effect of Whitmania Pigra Based On in Vitro Anticoagulation Experiment]. Zhongguo Zhong Yao Za Zhi. 2019;44(23):5114-5117. PubMed PMID: 32237346.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of lead pollution on anticoagulant effect of Whitmania pigra based on in vitro anticoagulation experiment]. AU - Luo,Xue-Mei, AU - Meng,Jie-Qin, AU - Chen,Xiu-Fen, AU - Xu,Qing, AU - Zhang,Fan, AU - Yang,Yao-Jun, PY - 2020/4/3/entrez PY - 2020/4/3/pubmed PY - 2020/5/2/medline KW - APTT KW - PT KW - Whitmania pigra KW - anticoagulant effect KW - lead pollution SP - 5114 EP - 5117 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 44 IS - 23 N2 - Leech has a good anticoagulant activity and is one of the raw materials for treatment of many cardiovascular and cerebrovascular diseases. This study was based on in vitro anticoagulant experiments( APTT and PT) to investigate the effects of lead contamination on the anticoagulant effect of leech. At present,the Hirudo circulating in the market are dominated by Whitmania pigra,therefore Wh. pigra were cultivated under a different lead pollution for 50 days. Then,the effects of Wh. pigra extract,extracting from different cultivating environment,on activated partial thrombin time( APTT) and prothrombin time( PT) were determined by automatic coagulation instrument. The results showed that the Wh. pigra extract significantly prolonged the APTT compared with the saline group.The APTT of the lead-high residual Wh. pigra was shorter than that of the blank Wh. pigra. The Wh. pigra extracts from different treatment groups had little effect on PT. The results showed that the lead residue in the Wh. pigra increased with the increase of lead in the cultured soil,the lead residual of the Pb-H group was( 10. 66±2. 79) mg·kg~(-1),which exceeded the lead limit specified in the 2015 edition of the Chinese Pharmacopoeia. The results indicated that growth environment pollution is one of the important factors causing excessive lead in Wh. pigra. Lead pollution will reduce the anticoagulant effect of Wh. pigra and affect its clinical efficacy. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/32237346/[Effect_of_lead_pollution_on_anticoagulant_effect_of_Whitmania_pigra_based_on_in_vitro_anticoagulation_experiment] DB - PRIME DP - Unbound Medicine ER -