Tags

Type your tag names separated by a space and hit enter

Transcriptomic profiling of microglia and astrocytes throughout aging.
J Neuroinflammation. 2020 Apr 01; 17(1):97.JN

Abstract

BACKGROUND

Activation of microglia and astrocytes, a prominent hallmark of both aging and Alzheimer's disease (AD), has been suggested to contribute to aging and AD progression, but the underlying cellular and molecular mechanisms are largely unknown.

METHODS

We performed RNA-seq analyses on microglia and astrocytes freshly isolated from wild-type and APP-PS1 (AD) mouse brains at five time points to elucidate their age-related gene-expression profiles.

RESULTS

Our results showed that from 4 months onward, a set of age-related genes in microglia and astrocytes exhibited consistent upregulation or downregulation (termed "age-up"/"age-down" genes) relative to their expression at the young-adult stage (2 months). And most age-up genes were more highly expressed in AD mice at the same time points. Bioinformatic analyses revealed that the age-up genes in microglia were associated with the inflammatory response, whereas these genes in astrocytes included widely recognized AD risk genes, genes associated with synaptic transmission or elimination, and peptidase-inhibitor genes.

CONCLUSIONS

Overall, our RNA-seq data provide a valuable resource for future investigations into the roles of microglia and astrocytes in aging- and amyloid-β-induced AD pathologies.

Links

PMC Free PDF

Authors+Show Affiliations

Pan J
Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China.
Ma N
Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China.
Yu B
Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China. Department of Dermatology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
Zhang W
Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China. zhangweispace@163.com.
Wan J
Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China. wanj@ust.hk. Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay Road, Kowloon, Hong Kong, China. wanj@ust.hk.

MeSH

AgingAlzheimer DiseaseAmyloid beta-Protein PrecursorAnimalsAstrocytesBrainDisease Models, AnimalDisease ProgressionGene Expression ProfilingMiceMice, TransgenicMicrogliaPresenilin-1Transcriptome

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32238175

Citation

Pan, Jie, et al. "Transcriptomic Profiling of Microglia and Astrocytes Throughout Aging." Journal of Neuroinflammation, vol. 17, no. 1, 2020, p. 97.
Pan J, Ma N, Yu B, et al. Transcriptomic profiling of microglia and astrocytes throughout aging. J Neuroinflammation. 2020;17(1):97.
Pan, J., Ma, N., Yu, B., Zhang, W., & Wan, J. (2020). Transcriptomic profiling of microglia and astrocytes throughout aging. Journal of Neuroinflammation, 17(1), 97. https://doi.org/10.1186/s12974-020-01774-9
Pan J, et al. Transcriptomic Profiling of Microglia and Astrocytes Throughout Aging. J Neuroinflammation. 2020 Apr 1;17(1):97. PubMed PMID: 32238175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptomic profiling of microglia and astrocytes throughout aging. AU - Pan,Jie, AU - Ma,Nana, AU - Yu,Bo, AU - Zhang,Wei, AU - Wan,Jun, Y1 - 2020/04/01/ PY - 2019/11/27/received PY - 2020/03/17/accepted PY - 2020/4/3/entrez PY - 2020/4/3/pubmed PY - 2021/1/7/medline KW - Aging KW - Alzheimer’s disease (AD) KW - Astrocyte KW - Microglia KW - RNA-seq SP - 97 EP - 97 JF - Journal of neuroinflammation JO - J Neuroinflammation VL - 17 IS - 1 N2 - BACKGROUND: Activation of microglia and astrocytes, a prominent hallmark of both aging and Alzheimer's disease (AD), has been suggested to contribute to aging and AD progression, but the underlying cellular and molecular mechanisms are largely unknown. METHODS: We performed RNA-seq analyses on microglia and astrocytes freshly isolated from wild-type and APP-PS1 (AD) mouse brains at five time points to elucidate their age-related gene-expression profiles. RESULTS: Our results showed that from 4 months onward, a set of age-related genes in microglia and astrocytes exhibited consistent upregulation or downregulation (termed "age-up"/"age-down" genes) relative to their expression at the young-adult stage (2 months). And most age-up genes were more highly expressed in AD mice at the same time points. Bioinformatic analyses revealed that the age-up genes in microglia were associated with the inflammatory response, whereas these genes in astrocytes included widely recognized AD risk genes, genes associated with synaptic transmission or elimination, and peptidase-inhibitor genes. CONCLUSIONS: Overall, our RNA-seq data provide a valuable resource for future investigations into the roles of microglia and astrocytes in aging- and amyloid-β-induced AD pathologies. SN - 1742-2094 UR - https://www.unboundmedicine.com/medline/citation/32238175/Transcriptomic_profiling_of_microglia_and_astrocytes_throughout_aging_ DB - PRIME DP - Unbound Medicine ER -