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Exceptional diversity of opsin expression patterns in Neogonodactylus oerstedii (Stomatopoda) retinas.
Proc Natl Acad Sci U S A. 2020 04 21; 117(16):8948-8957.PN

Abstract

Stomatopod crustaceans possess some of the most complex animal visual systems, including at least 16 spectrally distinct types of photoreceptive units (e.g., assemblages of photoreceptor cells). Here we fully characterize the set of opsin genes expressed in retinal tissues and determine expression patterns of each in the stomatopod Neogonodactylus oerstedii Using a combination of transcriptome and RACE sequencing, we identified 33 opsin transcripts expressed in each N. oerstedii eye, which are predicted to form 20 long-wavelength-sensitive, 10 middle-wavelength-sensitive, and three UV-sensitive visual pigments. Observed expression patterns of these 33 transcripts were highly unusual in five respects: 1) All long-wavelength and short/middle-wavelength photoreceptive units expressed multiple opsins, while UV photoreceptor cells expressed single opsins; 2) most of the long-wavelength photoreceptive units expressed at least one middle-wavelength-sensitive opsin transcript; 3) the photoreceptors involved in spatial, motion, and polarization vision expressed more transcripts than those involved in color vision; 4) there is a unique opsin transcript that is expressed in all eight of the photoreceptive units devoted to color vision; and 5) expression patterns in the peripheral hemispheres of the eyes suggest visual specializations not previously recognized in stomatopods. Elucidating the expression patterns of all opsin transcripts expressed in the N. oerstedii retina reveals the potential for previously undocumented functional diversity in the already complex stomatopod eye and is a first step toward understanding the functional significance of the unusual abundance of opsins found in many arthropod species' visual systems.

Authors+Show Affiliations

Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250; mlporter@hawaii.edu. Department of Biology, University of Hawai'i at Mānoa, Honolulu, HI 96822.Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250.Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250. School of Biological Sciences, University of Bristol, BS8 1TQ Bristol, United Kingdom.Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

32241889

Citation

Porter, Megan L., et al. "Exceptional Diversity of Opsin Expression Patterns in Neogonodactylus Oerstedii (Stomatopoda) Retinas." Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 16, 2020, pp. 8948-8957.
Porter ML, Awata H, Bok MJ, et al. Exceptional diversity of opsin expression patterns in Neogonodactylus oerstedii (Stomatopoda) retinas. Proc Natl Acad Sci U S A. 2020;117(16):8948-8957.
Porter, M. L., Awata, H., Bok, M. J., & Cronin, T. W. (2020). Exceptional diversity of opsin expression patterns in Neogonodactylus oerstedii (Stomatopoda) retinas. Proceedings of the National Academy of Sciences of the United States of America, 117(16), 8948-8957. https://doi.org/10.1073/pnas.1917303117
Porter ML, et al. Exceptional Diversity of Opsin Expression Patterns in Neogonodactylus Oerstedii (Stomatopoda) Retinas. Proc Natl Acad Sci U S A. 2020 04 21;117(16):8948-8957. PubMed PMID: 32241889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exceptional diversity of opsin expression patterns in Neogonodactylus oerstedii (Stomatopoda) retinas. AU - Porter,Megan L, AU - Awata,Hiroko, AU - Bok,Michael J, AU - Cronin,Thomas W, Y1 - 2020/04/02/ PY - 2020/4/4/pubmed PY - 2020/7/28/medline PY - 2020/4/4/entrez KW - Stomatopoda KW - evolution KW - in situ hybridization KW - opsin KW - retinal expression SP - 8948 EP - 8957 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 117 IS - 16 N2 - Stomatopod crustaceans possess some of the most complex animal visual systems, including at least 16 spectrally distinct types of photoreceptive units (e.g., assemblages of photoreceptor cells). Here we fully characterize the set of opsin genes expressed in retinal tissues and determine expression patterns of each in the stomatopod Neogonodactylus oerstedii Using a combination of transcriptome and RACE sequencing, we identified 33 opsin transcripts expressed in each N. oerstedii eye, which are predicted to form 20 long-wavelength-sensitive, 10 middle-wavelength-sensitive, and three UV-sensitive visual pigments. Observed expression patterns of these 33 transcripts were highly unusual in five respects: 1) All long-wavelength and short/middle-wavelength photoreceptive units expressed multiple opsins, while UV photoreceptor cells expressed single opsins; 2) most of the long-wavelength photoreceptive units expressed at least one middle-wavelength-sensitive opsin transcript; 3) the photoreceptors involved in spatial, motion, and polarization vision expressed more transcripts than those involved in color vision; 4) there is a unique opsin transcript that is expressed in all eight of the photoreceptive units devoted to color vision; and 5) expression patterns in the peripheral hemispheres of the eyes suggest visual specializations not previously recognized in stomatopods. Elucidating the expression patterns of all opsin transcripts expressed in the N. oerstedii retina reveals the potential for previously undocumented functional diversity in the already complex stomatopod eye and is a first step toward understanding the functional significance of the unusual abundance of opsins found in many arthropod species' visual systems. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/32241889/Exceptional_diversity_of_opsin_expression_patterns_in_Neogonodactylus_oerstedii__Stomatopoda__retinas_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=32241889 DB - PRIME DP - Unbound Medicine ER -