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Influence of hyaluronic acid binding on the actin cortex measured by optical forces.
J Biophotonics. 2020 Jul; 13(7):e201960215.JB

Abstract

Melanoma cells are often surrounded by hyaluronic acid (HA) rich environments, which are considered to promote tumor progression and metastasis. Induced effects in compound materials consisting of cells embedded in an extracellular matrix have been studied, however, alterations of the single cells have never been addressed. Here, we explicitly addressed single cell properties and measured HA-induced biomechanical changes via deformations induced solely by optical forces. With the optical stretcher setup, cells were deformed after culturing them in either the presence or absence of HA revealing the crucial interplay of HA with the CD44 receptor. To assess the role of CD44 in transducing effects of HA, we compared a CD44 expressing variant of the melanoma cell line RPM-MC to its natural CD44-negative counterpart. Our measurements revealed a significant stiffness change, which we attribute to changes of the actin cytoskeleton.

Authors+Show Affiliations

Leipzig University, Faculty of Physics and Earth Sciences, Peter Debye Institute for Soft Matter Physics, Leipzig, Germany. Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany.University of Maryland, Institute for Physical Science and Technology, Physical Sciences Complex, College Park, MD, USA.Department of Dermatology, Leipzig University, Medical Faculty, Leipzig, Germany.Leipzig University, Faculty of Physics and Earth Sciences, Peter Debye Institute for Soft Matter Physics, Leipzig, Germany.University of Maryland, Institute for Physical Science and Technology, Physical Sciences Complex, College Park, MD, USA.Department of Dermatology, Leipzig University, Medical Faculty, Leipzig, Germany.Leipzig University, Faculty of Physics and Earth Sciences, Peter Debye Institute for Soft Matter Physics, Leipzig, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32246559

Citation

Schnauβ, Jörg, et al. "Influence of Hyaluronic Acid Binding On the Actin Cortex Measured By Optical Forces." Journal of Biophotonics, vol. 13, no. 7, 2020, pp. e201960215.
Schnauβ J, Schmidt BUS, Brazel CB, et al. Influence of hyaluronic acid binding on the actin cortex measured by optical forces. J Biophotonics. 2020;13(7):e201960215.
Schnauβ, J., Schmidt, B. U. S., Brazel, C. B., Dogan, S., Losert, W., Anderegg, U., & Käs, J. A. (2020). Influence of hyaluronic acid binding on the actin cortex measured by optical forces. Journal of Biophotonics, 13(7), e201960215. https://doi.org/10.1002/jbio.201960215
Schnauβ J, et al. Influence of Hyaluronic Acid Binding On the Actin Cortex Measured By Optical Forces. J Biophotonics. 2020;13(7):e201960215. PubMed PMID: 32246559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of hyaluronic acid binding on the actin cortex measured by optical forces. AU - Schnauβ,Jörg, AU - Schmidt,B U Sebastian, AU - Brazel,Christina B, AU - Dogan,Senol, AU - Losert,Wolfgang, AU - Anderegg,Ulf, AU - Käs,Josef A, Y1 - 2020/04/14/ PY - 2019/12/10/received PY - 2020/03/24/revised PY - 2020/03/25/accepted PY - 2020/4/5/pubmed PY - 2020/4/5/medline PY - 2020/4/5/entrez KW - CD44 receptor KW - actin cytoskeleton KW - cell mechanics KW - hyaluronic acid KW - optical stretcher SP - e201960215 EP - e201960215 JF - Journal of biophotonics JO - J Biophotonics VL - 13 IS - 7 N2 - Melanoma cells are often surrounded by hyaluronic acid (HA) rich environments, which are considered to promote tumor progression and metastasis. Induced effects in compound materials consisting of cells embedded in an extracellular matrix have been studied, however, alterations of the single cells have never been addressed. Here, we explicitly addressed single cell properties and measured HA-induced biomechanical changes via deformations induced solely by optical forces. With the optical stretcher setup, cells were deformed after culturing them in either the presence or absence of HA revealing the crucial interplay of HA with the CD44 receptor. To assess the role of CD44 in transducing effects of HA, we compared a CD44 expressing variant of the melanoma cell line RPM-MC to its natural CD44-negative counterpart. Our measurements revealed a significant stiffness change, which we attribute to changes of the actin cytoskeleton. SN - 1864-0648 UR - https://www.unboundmedicine.com/medline/citation/32246559/Influence_of_hyaluronic_acid_binding_on_the_actin_cortex_measured_by_optical_forces L2 - https://doi.org/10.1002/jbio.201960215 DB - PRIME DP - Unbound Medicine ER -
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