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Enzyme-linked immunosorbent assay (ELISA) versus Venereal Disease Research Laboratory test (VDRL) and rapid plasma reagin test (RPR) for screening of syphilis in pregnant women.
Int J Gynaecol Obstet. 2020 Jul; 150(1):103-107.IJ

Abstract

OBJECTIVE

To evaluate a treponemal enzyme-linked immunosorbent assay (ELISA) as an alternative screening test for syphilis in pregnant women.

METHODS

A cross-sectional study of diagnostic test accuracy was carried out in a large volume laboratory from a tertiary care center. A total of 416 serum samples, including 102 archived syphilis Treponema pallidum hemagglutination (TPHA)-positive samples and 314 samples from pregnant women, were used to determine the sensitivity and specificity of ELISA. All the samples were subjected to Venereal Disease Research Laboratory (VDRL), rapid plasma reagin (RPR), ELISA, and TPHA tests. Performance characteristics of VDRL, RPR, and ELISA were calculated with TPHA as a reference standard test.

RESULTS

VDRL and RPR exhibited higher false positivity of 10.5% and 9.6%, respectively, compared to 2.5% by ELISA. The sensitivity and specificity of ELISA were 98% and 97.5%, of VDRL were 71.6% and 89.5%, and of RPR were 73.5% and 90.5%, respectively. Moreover, ELISA had an excellent agreement (kappa=0.9) with TPHA compared to VDRL/RPR which had a moderate agreement (kappa=0.6) only.

CONCLUSION

ELISA has the potential to replace VDRL/RPR as a screening test for syphilis in centers that can perform ELISA, especially for antenatal screening.

Authors+Show Affiliations

Department of Clinical Microbiology, Christian Medical College, Vellore, Tamil Nadu, India.Department of Obstetrics and Gynaecology, Christian Medical College, Vellore, Tamil Nadu, India.Department of Obstetrics and Gynaecology, Christian Medical College, Vellore, Tamil Nadu, India.Department of Obstetrics and Gynaecology, Christian Medical College, Vellore, Tamil Nadu, India.Department of Clinical Microbiology, Christian Medical College, Vellore, Tamil Nadu, India.Department of Clinical Microbiology, Christian Medical College, Vellore, Tamil Nadu, India.Department of Clinical Microbiology, Christian Medical College, Vellore, Tamil Nadu, India.

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

32246772

Citation

Solaimalai, Dhanalakshmi, et al. "Enzyme-linked Immunosorbent Assay (ELISA) Versus Venereal Disease Research Laboratory Test (VDRL) and Rapid Plasma Reagin Test (RPR) for Screening of Syphilis in Pregnant Women." International Journal of Gynaecology and Obstetrics: the Official Organ of the International Federation of Gynaecology and Obstetrics, vol. 150, no. 1, 2020, pp. 103-107.
Solaimalai D, Rathore S, Beck MM, et al. Enzyme-linked immunosorbent assay (ELISA) versus Venereal Disease Research Laboratory test (VDRL) and rapid plasma reagin test (RPR) for screening of syphilis in pregnant women. Int J Gynaecol Obstet. 2020;150(1):103-107.
Solaimalai, D., Rathore, S., Beck, M. M., Regi, A., Yesudhason, B. L., Veeraraghavan, B., & Prakash, J. A. J. (2020). Enzyme-linked immunosorbent assay (ELISA) versus Venereal Disease Research Laboratory test (VDRL) and rapid plasma reagin test (RPR) for screening of syphilis in pregnant women. International Journal of Gynaecology and Obstetrics: the Official Organ of the International Federation of Gynaecology and Obstetrics, 150(1), 103-107. https://doi.org/10.1002/ijgo.13154
Solaimalai D, et al. Enzyme-linked Immunosorbent Assay (ELISA) Versus Venereal Disease Research Laboratory Test (VDRL) and Rapid Plasma Reagin Test (RPR) for Screening of Syphilis in Pregnant Women. Int J Gynaecol Obstet. 2020;150(1):103-107. PubMed PMID: 32246772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzyme-linked immunosorbent assay (ELISA) versus Venereal Disease Research Laboratory test (VDRL) and rapid plasma reagin test (RPR) for screening of syphilis in pregnant women. AU - Solaimalai,Dhanalakshmi, AU - Rathore,Swati, AU - Beck,Manisha M, AU - Regi,Annie, AU - Yesudhason,Binesh L, AU - Veeraraghavan,Balaji, AU - Prakash,John A J, Y1 - 2020/04/23/ PY - 2019/11/04/received PY - 2020/02/26/revised PY - 2020/03/30/accepted PY - 2020/4/5/pubmed PY - 2020/9/30/medline PY - 2020/4/5/entrez KW - Enzyme-linked immunosorbent assay KW - Rapid plasma reagin KW - Screening KW - Sexually transmitted infections KW - Syphilis KW - Venereal Disease Research Laboratory SP - 103 EP - 107 JF - International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics JO - Int J Gynaecol Obstet VL - 150 IS - 1 N2 - OBJECTIVE: To evaluate a treponemal enzyme-linked immunosorbent assay (ELISA) as an alternative screening test for syphilis in pregnant women. METHODS: A cross-sectional study of diagnostic test accuracy was carried out in a large volume laboratory from a tertiary care center. A total of 416 serum samples, including 102 archived syphilis Treponema pallidum hemagglutination (TPHA)-positive samples and 314 samples from pregnant women, were used to determine the sensitivity and specificity of ELISA. All the samples were subjected to Venereal Disease Research Laboratory (VDRL), rapid plasma reagin (RPR), ELISA, and TPHA tests. Performance characteristics of VDRL, RPR, and ELISA were calculated with TPHA as a reference standard test. RESULTS: VDRL and RPR exhibited higher false positivity of 10.5% and 9.6%, respectively, compared to 2.5% by ELISA. The sensitivity and specificity of ELISA were 98% and 97.5%, of VDRL were 71.6% and 89.5%, and of RPR were 73.5% and 90.5%, respectively. Moreover, ELISA had an excellent agreement (kappa=0.9) with TPHA compared to VDRL/RPR which had a moderate agreement (kappa=0.6) only. CONCLUSION: ELISA has the potential to replace VDRL/RPR as a screening test for syphilis in centers that can perform ELISA, especially for antenatal screening. SN - 1879-3479 UR - https://www.unboundmedicine.com/medline/citation/32246772/Enzyme_linked_immunosorbent_assay__ELISA__versus_Venereal_Disease_Research_Laboratory_test__VDRL__and_rapid_plasma_reagin_test__RPR__for_screening_of_syphilis_in_pregnant_women_ L2 - https://doi.org/10.1002/ijgo.13154 DB - PRIME DP - Unbound Medicine ER -