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Sequential extraction and enrichment of flavonoids from Euonymus alatus by ultrasonic-assisted polyethylene glycol-based extraction coupled to temperature-induced cloud point extraction.
Ultrason Sonochem. 2020 Sep; 66:105073.US

Abstract

A green method for simultaneous extraction and enrichment of flavonoids from Euonymus alatus was developed by ultrasonic-assisted extraction (UAE) and temperature-induced cloud point extraction (TICPE) using PEG-base aqueous solution as the extractant. Based on screening different molecular weights of PEGs, PEG-400/water was used as the extractant, and the effects of key factors on extraction yields of flavonoids were investigated by single-factor experiments and response surface methodology (RSM). The optimum conditions of UAE were as follows: PEG-400 concentration of 16% (w/w), particle size of 80 mesh, solvent-to-material ratio of 60:1, extraction temperature of 90 °C and extraction time of 15 min. The results obtained by validation experiments were consistent with the values predicted by RSM. Temperature-induced formation of the aqueous two-phase system (ATPS) and TICPE process were further investigated by controlling temperature and adding (NH4)2SO4. In the presence of (NH4)2SO4, the ATPS formed at 75 ℃ and pH 3.5 could effectively improve separation and recovery of flavonoids with enrichment factor of above five times. Gallic acid, catechin, dihydromyricetin and ellagic acid in the extract were identified and confirmed by UPLC-Q-TOF-MS and the corresponding standards. The UAE-TICPE coupled to HPLC was successfully applied for extraction and determination of flavonoids in two batches of Euonymus alatus. The extraction yields of catechin, dihydromyricetin and total flavonoids were 0.377-0.684 mg/g, 1.091-1.353 mg/g and 2.612-3.146 mg/g, respectively. Compared to conventional extraction methods, PEG-based UAE integrated with TICPE in one-step procedure exhibited higher extraction efficiency and better extraction selectivity.

Authors+Show Affiliations

School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China; Guangdong Provincial Public Laboratory of Analysis and Testing Technology, China National Analytical Center, Guangzhou 510070, China.Guangdong Provincial Public Laboratory of Analysis and Testing Technology, China National Analytical Center, Guangzhou 510070, China.School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China.School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: junhuafan@126.com.School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.School of Food Engineering and Biotechnology, Guangdong Industry Polytechnic, Guangzhou 510300, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32247232

Citation

Mai, Xiaoman, et al. "Sequential Extraction and Enrichment of Flavonoids From Euonymus Alatus By Ultrasonic-assisted Polyethylene Glycol-based Extraction Coupled to Temperature-induced Cloud Point Extraction." Ultrasonics Sonochemistry, vol. 66, 2020, p. 105073.
Mai X, Liu Y, Tang X, et al. Sequential extraction and enrichment of flavonoids from Euonymus alatus by ultrasonic-assisted polyethylene glycol-based extraction coupled to temperature-induced cloud point extraction. Ultrason Sonochem. 2020;66:105073.
Mai, X., Liu, Y., Tang, X., Wang, L., Lin, Y., Zeng, H., Luo, L., Fan, H., & Li, P. (2020). Sequential extraction and enrichment of flavonoids from Euonymus alatus by ultrasonic-assisted polyethylene glycol-based extraction coupled to temperature-induced cloud point extraction. Ultrasonics Sonochemistry, 66, 105073. https://doi.org/10.1016/j.ultsonch.2020.105073
Mai X, et al. Sequential Extraction and Enrichment of Flavonoids From Euonymus Alatus By Ultrasonic-assisted Polyethylene Glycol-based Extraction Coupled to Temperature-induced Cloud Point Extraction. Ultrason Sonochem. 2020;66:105073. PubMed PMID: 32247232.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequential extraction and enrichment of flavonoids from Euonymus alatus by ultrasonic-assisted polyethylene glycol-based extraction coupled to temperature-induced cloud point extraction. AU - Mai,Xiaoman, AU - Liu,Yingtao, AU - Tang,Xunyou, AU - Wang,Liping, AU - Lin,Yuyang, AU - Zeng,Huiyun, AU - Luo,Laicheng, AU - Fan,Huajun, AU - Li,Pingfan, Y1 - 2020/03/27/ PY - 2019/12/10/received PY - 2020/03/13/revised PY - 2020/03/19/accepted PY - 2020/4/5/pubmed PY - 2021/1/8/medline PY - 2020/4/5/entrez KW - Euonymus alatus KW - Flavonoids KW - PEG-based extractant KW - Temperature-induced cloud-point extraction KW - Ultrasonic-assisted extraction SP - 105073 EP - 105073 JF - Ultrasonics sonochemistry JO - Ultrason Sonochem VL - 66 N2 - A green method for simultaneous extraction and enrichment of flavonoids from Euonymus alatus was developed by ultrasonic-assisted extraction (UAE) and temperature-induced cloud point extraction (TICPE) using PEG-base aqueous solution as the extractant. Based on screening different molecular weights of PEGs, PEG-400/water was used as the extractant, and the effects of key factors on extraction yields of flavonoids were investigated by single-factor experiments and response surface methodology (RSM). The optimum conditions of UAE were as follows: PEG-400 concentration of 16% (w/w), particle size of 80 mesh, solvent-to-material ratio of 60:1, extraction temperature of 90 °C and extraction time of 15 min. The results obtained by validation experiments were consistent with the values predicted by RSM. Temperature-induced formation of the aqueous two-phase system (ATPS) and TICPE process were further investigated by controlling temperature and adding (NH4)2SO4. In the presence of (NH4)2SO4, the ATPS formed at 75 ℃ and pH 3.5 could effectively improve separation and recovery of flavonoids with enrichment factor of above five times. Gallic acid, catechin, dihydromyricetin and ellagic acid in the extract were identified and confirmed by UPLC-Q-TOF-MS and the corresponding standards. The UAE-TICPE coupled to HPLC was successfully applied for extraction and determination of flavonoids in two batches of Euonymus alatus. The extraction yields of catechin, dihydromyricetin and total flavonoids were 0.377-0.684 mg/g, 1.091-1.353 mg/g and 2.612-3.146 mg/g, respectively. Compared to conventional extraction methods, PEG-based UAE integrated with TICPE in one-step procedure exhibited higher extraction efficiency and better extraction selectivity. SN - 1873-2828 UR - https://www.unboundmedicine.com/medline/citation/32247232/Sequential_extraction_and_enrichment_of_flavonoids_from_Euonymus_alatus_by_ultrasonic_assisted_polyethylene_glycol_based_extraction_coupled_to_temperature_induced_cloud_point_extraction_ DB - PRIME DP - Unbound Medicine ER -