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Spleen Oxylipin and Polyunsaturated Fatty Acid Profiles are Altered by Dietary Source of Polyunsaturated Fatty Acid and by Sex.
Lipids. 2020 05; 55(3):261-270.L

Abstract

As the largest secondary lymphoid organ, the spleen plays an important role in immune responses. The role of arachidonic acid (ARA) and its 20-carbon eicosanoids in modulating immune function has long been of interest. However, recent advances have enabled the identification of numerous other n-6 and n-3 polyunsaturated fatty acid (PUFA)-derived oxylipins. Here, we investigate the effects of diet and sex on the spleen nonesterified oxylipin profiles and phospholipid and neutral lipid PUFA composition in Sprague-Dawley rats supplemented with oils rich in α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), or linoleic acid. Dietary ALA, EPA, and DHA resulted in lower levels of ARA and ARA oxylipins. Oxylipins derived from other n-6 PUFA were also reduced despite no or opposite effect on their PUFA levels. Each diet also resulted in higher levels of oxylipins almost exclusively derived from the supplemented PUFA, despite PUFA in the same biosynthetic pathway also often being increased. Further, while oxylipin differences often reflected changes to phospholipid PUFA, there were instances where they corresponded more closely to changes in neutral lipid PUFA. With respect to sex effects, >50% of lipoxygenase ARA-derived oxylipins were higher in males in at least one diet group, while multiple DHA oxylipins were lower in males only in rats provided the DHA diet. This fundamental description of oxylipin composition in the spleen, including the influence of diet and sex and the relationship to PUFA composition, will help inform future studies examining the functions of these oxylipins under physiological and pathological conditions.

Authors+Show Affiliations

Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada. Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, Manitoba, Canada.Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada. Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, Manitoba, Canada.Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32255511

Citation

Pauls, Samantha D., et al. "Spleen Oxylipin and Polyunsaturated Fatty Acid Profiles Are Altered By Dietary Source of Polyunsaturated Fatty Acid and By Sex." Lipids, vol. 55, no. 3, 2020, pp. 261-270.
Pauls SD, Ragheb M, Winter T, et al. Spleen Oxylipin and Polyunsaturated Fatty Acid Profiles are Altered by Dietary Source of Polyunsaturated Fatty Acid and by Sex. Lipids. 2020;55(3):261-270.
Pauls, S. D., Ragheb, M., Winter, T., Leng, S., Taylor, C. G., Zahradka, P., & Aukema, H. M. (2020). Spleen Oxylipin and Polyunsaturated Fatty Acid Profiles are Altered by Dietary Source of Polyunsaturated Fatty Acid and by Sex. Lipids, 55(3), 261-270. https://doi.org/10.1002/lipd.12235
Pauls SD, et al. Spleen Oxylipin and Polyunsaturated Fatty Acid Profiles Are Altered By Dietary Source of Polyunsaturated Fatty Acid and By Sex. Lipids. 2020;55(3):261-270. PubMed PMID: 32255511.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spleen Oxylipin and Polyunsaturated Fatty Acid Profiles are Altered by Dietary Source of Polyunsaturated Fatty Acid and by Sex. AU - Pauls,Samantha D, AU - Ragheb,Mariam, AU - Winter,Tanja, AU - Leng,Shan, AU - Taylor,Carla G, AU - Zahradka,Peter, AU - Aukema,Harold M, Y1 - 2020/04/07/ PY - 2020/01/29/received PY - 2020/03/09/revised PY - 2020/03/18/accepted PY - 2020/4/8/pubmed PY - 2021/6/10/medline PY - 2020/4/8/entrez KW - Eicosanoids KW - Oxylipins KW - Spleen KW - Targeted Lipidomics KW - n-3 PUFA KW - n-6 PUFA SP - 261 EP - 270 JF - Lipids JO - Lipids VL - 55 IS - 3 N2 - As the largest secondary lymphoid organ, the spleen plays an important role in immune responses. The role of arachidonic acid (ARA) and its 20-carbon eicosanoids in modulating immune function has long been of interest. However, recent advances have enabled the identification of numerous other n-6 and n-3 polyunsaturated fatty acid (PUFA)-derived oxylipins. Here, we investigate the effects of diet and sex on the spleen nonesterified oxylipin profiles and phospholipid and neutral lipid PUFA composition in Sprague-Dawley rats supplemented with oils rich in α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), or linoleic acid. Dietary ALA, EPA, and DHA resulted in lower levels of ARA and ARA oxylipins. Oxylipins derived from other n-6 PUFA were also reduced despite no or opposite effect on their PUFA levels. Each diet also resulted in higher levels of oxylipins almost exclusively derived from the supplemented PUFA, despite PUFA in the same biosynthetic pathway also often being increased. Further, while oxylipin differences often reflected changes to phospholipid PUFA, there were instances where they corresponded more closely to changes in neutral lipid PUFA. With respect to sex effects, >50% of lipoxygenase ARA-derived oxylipins were higher in males in at least one diet group, while multiple DHA oxylipins were lower in males only in rats provided the DHA diet. This fundamental description of oxylipin composition in the spleen, including the influence of diet and sex and the relationship to PUFA composition, will help inform future studies examining the functions of these oxylipins under physiological and pathological conditions. SN - 1558-9307 UR - https://www.unboundmedicine.com/medline/citation/32255511/Spleen_Oxylipin_and_Polyunsaturated_Fatty_Acid_Profiles_are_Altered_by_Dietary_Source_of_Polyunsaturated_Fatty_Acid_and_by_Sex_ L2 - https://doi.org/10.1002/lipd.12235 DB - PRIME DP - Unbound Medicine ER -