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Rhizosphere bacterial and fungal communities during the growth of Angelica sinensis seedlings cultivated in an Alpine uncultivated meadow soil.
PeerJ. 2020; 8:e8541.P

Abstract

Background

Angelica sinensis seedlings are grown in alpine uncultivated meadow soil with rainfed agroecosystems to ensure the quality of A. sinensis after seedling transplantation. The aim was to investigate the rhizosphere bacterial and fungal communities during the growth stages of A. sinensis seedlings.

Methods

The bacterial and fungal communities were investigated by HiSeq sequencing of 16S and 18S rDNA, respectively.

Results

Proteobacteria and Bacteroidetes were bacterial dominant phyla throughout growth stages. Fungal dominant phyla varied with growth stages, dominant phyla Ascomycota and Chytridiomycota in AM5, dominant phyla Basidiomycota, Ascomycota and Zygomycota in BM5, and dominant phyla Basidiomycota and Ascomycota in CM5. There was no significant variation in the alpha-diversity of the bacterial and fungal communities, but significant variation was in the beta-diversity. We found that the variation of microbial community composition was accompanied by the changes in community function. The relative abundance of fungal pathogens increased with plant growth. We also identified the core microbes, significant-changing microbes, stage-specific microbes, and host-specific microbes. Plant weight, root length, root diameter, soil pH, rainfall, and climate temperature were the key divers to microbial community composition.

Conclusions

Our findings reported the variation and environmental drivers of rhizosphere bacterial and fungal communities during the growth of A. sinensis seedlings, which enhance the understanding of the rhizosphere microbial community in this habitat.

Authors+Show Affiliations

College of Life Science and Technology, College of Agronomy, Gansu Provincial Key Lab of Good Agricultural Production for Traditional Chinese Medicine, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China. Pharmacy Department, Gansu University of Chinese Medicine, Dingxi, China.College of Life Science and Technology, College of Agronomy, Gansu Provincial Key Lab of Good Agricultural Production for Traditional Chinese Medicine, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China.College of Life Science and Technology, College of Agronomy, Gansu Provincial Key Lab of Good Agricultural Production for Traditional Chinese Medicine, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China. Gansu Engineering Lab of Resource Reservation and Utilization for Characteristic Chinese Medicine, Gansu Tasly Zhongtian Pharmaceutical Co., Ltd., Dingxi, China.College of Life Science and Technology, College of Agronomy, Gansu Provincial Key Lab of Good Agricultural Production for Traditional Chinese Medicine, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China.College of Life Science and Technology, College of Agronomy, Gansu Provincial Key Lab of Good Agricultural Production for Traditional Chinese Medicine, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32257632

Citation

An, Zhigang, et al. "Rhizosphere Bacterial and Fungal Communities During the Growth of Angelica Sinensis Seedlings Cultivated in an Alpine Uncultivated Meadow Soil." PeerJ, vol. 8, 2020, pp. e8541.
An Z, Guo F, Chen Y, et al. Rhizosphere bacterial and fungal communities during the growth of Angelica sinensis seedlings cultivated in an Alpine uncultivated meadow soil. PeerJ. 2020;8:e8541.
An, Z., Guo, F., Chen, Y., Bai, G., & Chen, Z. (2020). Rhizosphere bacterial and fungal communities during the growth of Angelica sinensis seedlings cultivated in an Alpine uncultivated meadow soil. PeerJ, 8, e8541. https://doi.org/10.7717/peerj.8541
An Z, et al. Rhizosphere Bacterial and Fungal Communities During the Growth of Angelica Sinensis Seedlings Cultivated in an Alpine Uncultivated Meadow Soil. PeerJ. 2020;8:e8541. PubMed PMID: 32257632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rhizosphere bacterial and fungal communities during the growth of Angelica sinensis seedlings cultivated in an Alpine uncultivated meadow soil. AU - An,Zhigang, AU - Guo,Fengxia, AU - Chen,Yuan, AU - Bai,Gang, AU - Chen,Zhengjun, Y1 - 2020/03/26/ PY - 2019/07/29/received PY - 2020/01/09/accepted PY - 2020/4/8/entrez PY - 2020/4/8/pubmed PY - 2020/4/8/medline KW - Angelica sinensis KW - Bacteria KW - Community function KW - Diversity KW - Fungi KW - Rhizosphere KW - Seedlings SP - e8541 EP - e8541 JF - PeerJ JO - PeerJ VL - 8 N2 - Background: Angelica sinensis seedlings are grown in alpine uncultivated meadow soil with rainfed agroecosystems to ensure the quality of A. sinensis after seedling transplantation. The aim was to investigate the rhizosphere bacterial and fungal communities during the growth stages of A. sinensis seedlings. Methods: The bacterial and fungal communities were investigated by HiSeq sequencing of 16S and 18S rDNA, respectively. Results: Proteobacteria and Bacteroidetes were bacterial dominant phyla throughout growth stages. Fungal dominant phyla varied with growth stages, dominant phyla Ascomycota and Chytridiomycota in AM5, dominant phyla Basidiomycota, Ascomycota and Zygomycota in BM5, and dominant phyla Basidiomycota and Ascomycota in CM5. There was no significant variation in the alpha-diversity of the bacterial and fungal communities, but significant variation was in the beta-diversity. We found that the variation of microbial community composition was accompanied by the changes in community function. The relative abundance of fungal pathogens increased with plant growth. We also identified the core microbes, significant-changing microbes, stage-specific microbes, and host-specific microbes. Plant weight, root length, root diameter, soil pH, rainfall, and climate temperature were the key divers to microbial community composition. Conclusions: Our findings reported the variation and environmental drivers of rhizosphere bacterial and fungal communities during the growth of A. sinensis seedlings, which enhance the understanding of the rhizosphere microbial community in this habitat. SN - 2167-8359 UR - https://www.unboundmedicine.com/medline/citation/32257632/Rhizosphere_bacterial_and_fungal_communities_during_the_growth_of_Angelica_sinensis_seedlings_cultivated_in_an_Alpine_uncultivated_meadow_soil. L2 - https://doi.org/10.7717/peerj.8541 DB - PRIME DP - Unbound Medicine ER -
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