Citation
Chu, Hin, et al. "Comparative Replication and Immune Activation Profiles of SARS-CoV-2 and SARS-CoV in Human Lungs: an Ex Vivo Study With Implications for the Pathogenesis of COVID-19." Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America, vol. 71, no. 6, 2020, pp. 1400-1409.
Chu H, Chan JF, Wang Y, et al. Comparative Replication and Immune Activation Profiles of SARS-CoV-2 and SARS-CoV in Human Lungs: An Ex Vivo Study With Implications for the Pathogenesis of COVID-19. Clin Infect Dis. 2020;71(6):1400-1409.
Chu, H., Chan, J. F., Wang, Y., Yuen, T. T., Chai, Y., Hou, Y., Shuai, H., Yang, D., Hu, B., Huang, X., Zhang, X., Cai, J. P., Zhou, J., Yuan, S., Kok, K. H., To, K. K., Chan, I. H., Zhang, A. J., Sit, K. Y., ... Yuen, K. Y. (2020). Comparative Replication and Immune Activation Profiles of SARS-CoV-2 and SARS-CoV in Human Lungs: An Ex Vivo Study With Implications for the Pathogenesis of COVID-19. Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America, 71(6), 1400-1409. https://doi.org/10.1093/cid/ciaa410
Chu H, et al. Comparative Replication and Immune Activation Profiles of SARS-CoV-2 and SARS-CoV in Human Lungs: an Ex Vivo Study With Implications for the Pathogenesis of COVID-19. Clin Infect Dis. 2020 09 12;71(6):1400-1409. PubMed PMID: 32270184.
TY - JOUR
T1 - Comparative Replication and Immune Activation Profiles of SARS-CoV-2 and SARS-CoV in Human Lungs: An Ex Vivo Study With Implications for the Pathogenesis of COVID-19.
AU - Chu,Hin,
AU - Chan,Jasper Fuk-Woo,
AU - Wang,Yixin,
AU - Yuen,Terrence Tsz-Tai,
AU - Chai,Yue,
AU - Hou,Yuxin,
AU - Shuai,Huiping,
AU - Yang,Dong,
AU - Hu,Bingjie,
AU - Huang,Xiner,
AU - Zhang,Xi,
AU - Cai,Jian-Piao,
AU - Zhou,Jie,
AU - Yuan,Shuofeng,
AU - Kok,Kin-Hang,
AU - To,Kelvin Kai-Wang,
AU - Chan,Ivy Hau-Yee,
AU - Zhang,Anna Jinxia,
AU - Sit,Ko-Yung,
AU - Au,Wing-Kuk,
AU - Yuen,Kwok-Yung,
PY - 2020/04/04/received
PY - 2020/04/08/accepted
PY - 2020/4/10/pubmed
PY - 2020/9/25/medline
PY - 2020/4/10/entrez
KW - COVID-19
KW - SARS-CoV-2
KW - coronavirus
KW - ex vivo
KW - interferon
SP - 1400
EP - 1409
JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
JO - Clin Infect Dis
VL - 71
IS - 6
N2 - BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging coronavirus that has resulted in more than 2 000 000 laboratory-confirmed cases including over 145 000 deaths. Although SARS-CoV-2 and SARS-CoV share a number of common clinical manifestations, SARS-CoV-2 appears to be highly efficient in person-to-person transmission and frequently causes asymptomatic or presymptomatic infections. However, the underlying mechanisms that confer these viral characteristics of high transmissibility and asymptomatic infection remain incompletely understood. METHODS: We comprehensively investigated the replication, cell tropism, and immune activation profile of SARS-CoV-2 infection in human lung tissues with SARS-CoV included as a comparison. RESULTS: SARS-CoV-2 infected and replicated in human lung tissues more efficiently than SARS-CoV. Within the 48-hour interval, SARS-CoV-2 generated 3.20-fold more infectious virus particles than did SARS-CoV from the infected lung tissues (P < .024). SARS-CoV-2 and SARS-CoV were similar in cell tropism, with both targeting types I and II pneumocytes and alveolar macrophages. Importantly, despite the more efficient virus replication, SARS-CoV-2 did not significantly induce types I, II, or III interferons in the infected human lung tissues. In addition, while SARS-CoV infection upregulated the expression of 11 out of 13 (84.62%) representative proinflammatory cytokines/chemokines, SARS-CoV-2 infection only upregulated 5 of these 13 (38.46%) key inflammatory mediators despite replicating more efficiently. CONCLUSIONS: Our study provides the first quantitative data on the comparative replication capacity and immune activation profile of SARS-CoV-2 and SARS-CoV infection in human lung tissues. Our results provide important insights into the pathogenesis, high transmissibility, and asymptomatic infection of SARS-CoV-2.
SN - 1537-6591
UR - https://www.unboundmedicine.com/medline/citation/32270184/Comparative_Replication_and_Immune_Activation_Profiles_of_SARS_CoV_2_and_SARS_CoV_in_Human_Lungs:_An_Ex_Vivo_Study_With_Implications_for_the_Pathogenesis_of_COVID_19_
L2 - https://academic.oup.com/cid/article-lookup/doi/10.1093/cid/ciaa410
DB - PRIME
DP - Unbound Medicine
ER -