Tags

Type your tag names separated by a space and hit enter

Improve the developmental competence of porcine oocytes from small antral follicles by pre-maturation culture method.
Theriogenology. 2020 Jun; 149:139-148.T

Abstract

The oocytes from small antral follicle have low developmental potential to reach blastocyst due to incomplete cytoplasmic maturation during in vitro maturation (IVM). Thus, we developed an in vitro culture system for porcine oocytes derived from small antral follicles with l-ascorbic acid supplement during pre-maturation (pre-IVM) to support their development to blastocyst stage. Besides that, how l-ascorbic acid effect on the developmental competence of porcine oocytes with a special focus on histone modifications will be elucidated. The in vitro culture process consisted of two steps. The first step is 22 h of pre-IVM and the second step is 42 h of IVM. We utilized dibutyryl-cyclicAMP (dbcAMP) with L-ascorbic supplement during pre-IVM. Based on the result of this procedure, we proposed that the best culture condition in which hormone human chorionic gonadotropin (hCG) be added during the last 7 h of pre-IVM and continued culture to complete IVM. We observed that, in this culture system, the meiotic competence of porcine oocytes derived from small follicles was as high as those derived from large follicles after undergoing IVM. In addition, our study suggested that l-ascorbic acid supplementation at 100 μg/mL sharply enhanced the developmental potential of porcine oocytes. Interestingly, oocytes from small antral follicles treated with l-ascorbic acid could obtain the blastocyst quantity and quality as high as that of large antral follicles. The treated groups showed a significantly higher number of blastomeres compared to those in non-treated groups in both small and large follicle groups. Besides that, = The increasing levels of acetylation of histone H3 at lysine 9 (H3K9) and methylation of histone H3 at lysine 4 (H3K4) in blastocyst derived from small and large antral follicle under the present of l-ascrobic acid lead to a significant positive effect on the developmental competence and improvement in quality of porcine embryos.

Authors+Show Affiliations

Cellular Reprogramming Laboratory, School of Biotechnology, International University, Ho Chi Minh City, Vietnam; Vietnam National University, Ho Chi Minh City, Vietnam.Cellular Reprogramming Laboratory, School of Biotechnology, International University, Ho Chi Minh City, Vietnam; Vietnam National University, Ho Chi Minh City, Vietnam.Cellular Reprogramming Laboratory, School of Biotechnology, International University, Ho Chi Minh City, Vietnam; Vietnam National University, Ho Chi Minh City, Vietnam. Electronic address: nvthuan@hcmiu.edu.vn.Cellular Reprogramming Laboratory, School of Biotechnology, International University, Ho Chi Minh City, Vietnam; Vietnam National University, Ho Chi Minh City, Vietnam. Electronic address: bhthuy@hcmiu.edu.vn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32272343

Citation

Thuy Van, Nguyen Thi, et al. "Improve the Developmental Competence of Porcine Oocytes From Small Antral Follicles By Pre-maturation Culture Method." Theriogenology, vol. 149, 2020, pp. 139-148.
Thuy Van NT, Anh My LB, Van Thuan N, et al. Improve the developmental competence of porcine oocytes from small antral follicles by pre-maturation culture method. Theriogenology. 2020;149:139-148.
Thuy Van, N. T., Anh My, L. B., Van Thuan, N., & Bui, H. T. (2020). Improve the developmental competence of porcine oocytes from small antral follicles by pre-maturation culture method. Theriogenology, 149, 139-148. https://doi.org/10.1016/j.theriogenology.2020.02.038
Thuy Van NT, et al. Improve the Developmental Competence of Porcine Oocytes From Small Antral Follicles By Pre-maturation Culture Method. Theriogenology. 2020;149:139-148. PubMed PMID: 32272343.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improve the developmental competence of porcine oocytes from small antral follicles by pre-maturation culture method. AU - Thuy Van,Nguyen Thi, AU - Anh My,Le Ba, AU - Van Thuan,Nguyen, AU - Bui,Hong-Thuy, Y1 - 2020/03/04/ PY - 2019/11/15/received PY - 2020/02/05/revised PY - 2020/02/24/accepted PY - 2020/4/10/pubmed PY - 2020/4/10/medline PY - 2020/4/10/entrez KW - Histone acetylation KW - Histone methylation KW - Porcine oocyte KW - Small antral follicle KW - l-ascorbic acid SP - 139 EP - 148 JF - Theriogenology JO - Theriogenology VL - 149 N2 - The oocytes from small antral follicle have low developmental potential to reach blastocyst due to incomplete cytoplasmic maturation during in vitro maturation (IVM). Thus, we developed an in vitro culture system for porcine oocytes derived from small antral follicles with l-ascorbic acid supplement during pre-maturation (pre-IVM) to support their development to blastocyst stage. Besides that, how l-ascorbic acid effect on the developmental competence of porcine oocytes with a special focus on histone modifications will be elucidated. The in vitro culture process consisted of two steps. The first step is 22 h of pre-IVM and the second step is 42 h of IVM. We utilized dibutyryl-cyclicAMP (dbcAMP) with L-ascorbic supplement during pre-IVM. Based on the result of this procedure, we proposed that the best culture condition in which hormone human chorionic gonadotropin (hCG) be added during the last 7 h of pre-IVM and continued culture to complete IVM. We observed that, in this culture system, the meiotic competence of porcine oocytes derived from small follicles was as high as those derived from large follicles after undergoing IVM. In addition, our study suggested that l-ascorbic acid supplementation at 100 μg/mL sharply enhanced the developmental potential of porcine oocytes. Interestingly, oocytes from small antral follicles treated with l-ascorbic acid could obtain the blastocyst quantity and quality as high as that of large antral follicles. The treated groups showed a significantly higher number of blastomeres compared to those in non-treated groups in both small and large follicle groups. Besides that, = The increasing levels of acetylation of histone H3 at lysine 9 (H3K9) and methylation of histone H3 at lysine 4 (H3K4) in blastocyst derived from small and large antral follicle under the present of l-ascrobic acid lead to a significant positive effect on the developmental competence and improvement in quality of porcine embryos. SN - 1879-3231 UR - https://www.unboundmedicine.com/medline/citation/32272343/Improve_the_developmental_competence_of_porcine_oocytes_from_small_antral_follicles_by_pre-maturation_culture_method L2 - https://linkinghub.elsevier.com/retrieve/pii/S0093-691X(20)30150-3 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.