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High Performance Liquid Chromatography and Metabolomics Analysis of Tannase Metabolism of Gallic Acid and Gallates in Tea Leaves.
J Agric Food Chem. 2020 Apr 29; 68(17):4946-4954.JA

Abstract

Tannase (E.C. 3.1.1.20) is hypothesized to be involved in the metabolism of gallates and gallic acid (GA) in pu-erh tea fermentation. In this work, we measured tannase in Aspergillus niger fermented tea leaves and confirmed the production of fungal tannase during pu-erh tea fermentation. A decrease in catechin and theaflavin gallates and a significant increase in GA content and the relative peak areas of ethyl gallate, procyanidin A2, procyanidin B2, procyanidin B3, catechin-catechin-catechin, epiafzelechin, and epicatechin-epiafzelechin [variable importance in the projection (VIP) > 1.0, p < 0.05, and fold change (FC) > 1.5] were observed using high performance liquid chromatography (HPLC) and metabolomics analysis of tea leaves fermented or hydrolyzed by tannase. In vitro assays showed that hydrolysis by tannase or polymerization of catechins increased the antioxidant activity of tea leaves. In summary, we identified a metabolic pathway for gallates and their derivatives in tea leaves hydrolyzed by tannase as well as associated changes in gallate and GA concentrations caused by fungal tannase during pu-erh tea fermentation.

Authors+Show Affiliations

College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China.State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, 230036 Anhui China.College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China.College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China.College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China.College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China.College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China.College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China.College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China.College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China.College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China.College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China.State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, 230036 Anhui China.College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China. State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, China. The Key Laboratory of Medicinal Plant Biology of Yunnan Province, National & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming, Yunnan 650201, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32275834

Citation

Liu, Mingli, et al. "High Performance Liquid Chromatography and Metabolomics Analysis of Tannase Metabolism of Gallic Acid and Gallates in Tea Leaves." Journal of Agricultural and Food Chemistry, vol. 68, no. 17, 2020, pp. 4946-4954.
Liu M, Xie H, Ma Y, et al. High Performance Liquid Chromatography and Metabolomics Analysis of Tannase Metabolism of Gallic Acid and Gallates in Tea Leaves. J Agric Food Chem. 2020;68(17):4946-4954.
Liu, M., Xie, H., Ma, Y., Li, H., Li, C., Chen, L., Jiang, B., Nian, B., Guo, T., Zhang, Z., Jiao, W., Liu, Q., Ling, T., & Zhao, M. (2020). High Performance Liquid Chromatography and Metabolomics Analysis of Tannase Metabolism of Gallic Acid and Gallates in Tea Leaves. Journal of Agricultural and Food Chemistry, 68(17), 4946-4954. https://doi.org/10.1021/acs.jafc.0c00513
Liu M, et al. High Performance Liquid Chromatography and Metabolomics Analysis of Tannase Metabolism of Gallic Acid and Gallates in Tea Leaves. J Agric Food Chem. 2020 Apr 29;68(17):4946-4954. PubMed PMID: 32275834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High Performance Liquid Chromatography and Metabolomics Analysis of Tannase Metabolism of Gallic Acid and Gallates in Tea Leaves. AU - Liu,Mingli, AU - Xie,Haofen, AU - Ma,Yan, AU - Li,Hongye, AU - Li,Chongping, AU - Chen,Lijiao, AU - Jiang,Bin, AU - Nian,Bo, AU - Guo,Tianjie, AU - Zhang,Zhengyan, AU - Jiao,Wenwen, AU - Liu,Qianting, AU - Ling,Tiejun, AU - Zhao,Ming, Y1 - 2020/04/17/ PY - 2020/4/11/pubmed PY - 2020/12/30/medline PY - 2020/4/11/entrez KW - antioxidant activity KW - gallate KW - gallic acid KW - metabolomics analysis KW - pu-erh tea KW - tannase SP - 4946 EP - 4954 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 68 IS - 17 N2 - Tannase (E.C. 3.1.1.20) is hypothesized to be involved in the metabolism of gallates and gallic acid (GA) in pu-erh tea fermentation. In this work, we measured tannase in Aspergillus niger fermented tea leaves and confirmed the production of fungal tannase during pu-erh tea fermentation. A decrease in catechin and theaflavin gallates and a significant increase in GA content and the relative peak areas of ethyl gallate, procyanidin A2, procyanidin B2, procyanidin B3, catechin-catechin-catechin, epiafzelechin, and epicatechin-epiafzelechin [variable importance in the projection (VIP) > 1.0, p < 0.05, and fold change (FC) > 1.5] were observed using high performance liquid chromatography (HPLC) and metabolomics analysis of tea leaves fermented or hydrolyzed by tannase. In vitro assays showed that hydrolysis by tannase or polymerization of catechins increased the antioxidant activity of tea leaves. In summary, we identified a metabolic pathway for gallates and their derivatives in tea leaves hydrolyzed by tannase as well as associated changes in gallate and GA concentrations caused by fungal tannase during pu-erh tea fermentation. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/32275834/High_Performance_Liquid_Chromatography_and_Metabolomics_Analysis_of_Tannase_Metabolism_of_Gallic_Acid_and_Gallates_in_Tea_Leaves_ L2 - https://doi.org/10.1021/acs.jafc.0c00513 DB - PRIME DP - Unbound Medicine ER -