Citation
Yan, C, et al. "Rapid and Visual Detection of 2019 Novel Coronavirus (SARS-CoV-2) By a Reverse Transcription Loop-mediated Isothermal Amplification Assay." Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, vol. 26, no. 6, 2020, pp. 773-779.
Yan C, Cui J, Huang L, et al. Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay. Clin Microbiol Infect. 2020;26(6):773-779.
Yan, C., Cui, J., Huang, L., Du, B., Chen, L., Xue, G., Li, S., Zhang, W., Zhao, L., Sun, Y., Yao, H., Li, N., Zhao, H., Feng, Y., Liu, S., Zhang, Q., Liu, D., & Yuan, J. (2020). Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay. Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 26(6), 773-779. https://doi.org/10.1016/j.cmi.2020.04.001
Yan C, et al. Rapid and Visual Detection of 2019 Novel Coronavirus (SARS-CoV-2) By a Reverse Transcription Loop-mediated Isothermal Amplification Assay. Clin Microbiol Infect. 2020;26(6):773-779. PubMed PMID: 32276116.
TY - JOUR
T1 - Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay.
AU - Yan,C,
AU - Cui,J,
AU - Huang,L,
AU - Du,B,
AU - Chen,L,
AU - Xue,G,
AU - Li,S,
AU - Zhang,W,
AU - Zhao,L,
AU - Sun,Y,
AU - Yao,H,
AU - Li,N,
AU - Zhao,H,
AU - Feng,Y,
AU - Liu,S,
AU - Zhang,Q,
AU - Liu,D,
AU - Yuan,J,
Y1 - 2020/04/08/
PY - 2020/03/03/received
PY - 2020/04/01/revised
PY - 2020/04/02/accepted
PY - 2020/4/11/pubmed
PY - 2020/6/2/medline
PY - 2020/4/11/entrez
KW - COVID-19
KW - Detection
KW - RT-LAMP
KW - SARS-CoV-2
KW - Visual
SP - 773
EP - 779
JF - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
JO - Clin. Microbiol. Infect.
VL - 26
IS - 6
N2 - OBJECTIVE: To evaluate a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and compare it with RT-PCR. METHODS: We designed primers specific to the orf1ab and S genes of SARS-CoV-2. Total viral RNA was extracted using the QIAamp Viral RNA Mini Kit. We optimized the RT-LAMP assay, and evaluated it for its sensitivity and specificity of detection using real-time turbidity monitoring and visual observation. RESULTS: The primer sets orf1ab-4 and S-123 amplified the genes in the shortest times, the mean (±SD) times were 18 ± 1.32 min and 20 ± 1.80 min, respectively, and 63°C was the optimum reaction temperature. The sensitivities were 2 × 101 copies and 2 × 102 copies per reaction with primer sets orf1ab-4 and S-123, respectively. This assay showed no cross-reactivity with 60 other respiratory pathogens. To describe the availability of this method in clinical diagnosis, we collected 130 specimens from patients with clinically suspected SARS-CoV-2 infection. Among them, 58 were confirmed to be positive and 72 were negative by RT-LAMP. The sensitivity was 100% (95% CI 92.3%-100%), specificity 100% (95% CI 93.7%-100%). This assay detected SARS-CoV-2 in a mean (±SD) time of 26.28 ± 4.48 min and the results can be identified with visual observation. CONCLUSION: These results demonstrate that we developed a rapid, simple, specific and sensitive RT-LAMP assay for SARS-CoV-2 detection among clinical samples. It will be a powerful tool for SARS-CoV-2 identification, and for monitoring suspected patients, close contacts and high-risk groups.
SN - 1469-0691
UR - https://www.unboundmedicine.com/medline/citation/32276116/Rapid_and_visual_detection_of_2019_novel_coronavirus__SARS_CoV_2__by_a_reverse_transcription_loop_mediated_isothermal_amplification_assay_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(20)30186-5
DB - PRIME
DP - Unbound Medicine
ER -