Tags

Type your tag names separated by a space and hit enter

Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development.
Sci Rep. 2020 Apr 10; 10(1):6255.SR

Abstract

In artificial insemination the use of sex-sorted bovine sperm results in reduced conception, the causes of which are only partly understood. Therefore, we set out to investigate the effects of sexing on bovine sperm function and early embryonic development. Computer-assisted semen analysis (CASA) of sperm of the same bulls (n = 5), before and after sexing, demonstrated significantly reduced fast (A) and slow (B) progressively motile sperm (p < 0.05) after sexing. Sexed-sperm also revealed significantly less hyperactivated sperm (p < 0.05). As shown by time-lapse videomicroscopy of in vitro produced embryos (n = 360), embryos derived from sexed-sperm displayed significantly increased incidences of arrest at the 4-cell stage (p < 0.05). The relative risk for shrinkage/fusion of blastomeres with subsequent lysis was 1.71 times higher in the embryos derived from sexed-sperm as compared to conventional embryos (p < 0.05) resulting in significantly reduced blastocyst rates (p < 0.001). The relative risk for cleavage was 2.36 times lower in the embryos derived from sex-sorted sperm (p < 0.001). Additionally, sexed-sperm-derived embryos showed reduced survival times (hazard ratio HR = 1.54, p < 0.001) which were bull dependent (p < 0.001). However, the percentage of apoptotic cells was similar to conventional embryos. Furthermore, embryos derived from sexed-sperm were found to reach developmental stages at similar timings as conventional embryos. Our results suggest that reduced conception rates after sexing are due to altered sperm morphokinetics, decreasing the chance of sperm to reach and fertilise the oocyte, and aberrant early embryonic development.

Authors+Show Affiliations

School of Medicine, Health Sciences Centre, University College Dublin, Dublin, Ireland.School of Medicine, University of Nottingham, Nottingham, United Kingdom.School of Medicine, University of Nottingham, Nottingham, United Kingdom.School of Veterinary Medicine, Institute of Veterinary Anatomy, Histology and Embryology, University of Munich, Munich, Germany.School of Medicine, Health Sciences Centre, University College Dublin, Dublin, Ireland. sabine.koelle@ucd.ie.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32277124

Citation

Steele, H, et al. "Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development." Scientific Reports, vol. 10, no. 1, 2020, p. 6255.
Steele H, Makri D, Maalouf WE, et al. Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development. Sci Rep. 2020;10(1):6255.
Steele, H., Makri, D., Maalouf, W. E., Reese, S., & Kölle, S. (2020). Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development. Scientific Reports, 10(1), 6255. https://doi.org/10.1038/s41598-020-63077-6
Steele H, et al. Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development. Sci Rep. 2020 Apr 10;10(1):6255. PubMed PMID: 32277124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development. AU - Steele,H, AU - Makri,D, AU - Maalouf,W E, AU - Reese,S, AU - Kölle,S, Y1 - 2020/04/10/ PY - 2019/10/27/received PY - 2020/03/20/accepted PY - 2020/4/12/entrez PY - 2020/4/12/pubmed PY - 2020/4/12/medline SP - 6255 EP - 6255 JF - Scientific reports JO - Sci Rep VL - 10 IS - 1 N2 - In artificial insemination the use of sex-sorted bovine sperm results in reduced conception, the causes of which are only partly understood. Therefore, we set out to investigate the effects of sexing on bovine sperm function and early embryonic development. Computer-assisted semen analysis (CASA) of sperm of the same bulls (n = 5), before and after sexing, demonstrated significantly reduced fast (A) and slow (B) progressively motile sperm (p < 0.05) after sexing. Sexed-sperm also revealed significantly less hyperactivated sperm (p < 0.05). As shown by time-lapse videomicroscopy of in vitro produced embryos (n = 360), embryos derived from sexed-sperm displayed significantly increased incidences of arrest at the 4-cell stage (p < 0.05). The relative risk for shrinkage/fusion of blastomeres with subsequent lysis was 1.71 times higher in the embryos derived from sexed-sperm as compared to conventional embryos (p < 0.05) resulting in significantly reduced blastocyst rates (p < 0.001). The relative risk for cleavage was 2.36 times lower in the embryos derived from sex-sorted sperm (p < 0.001). Additionally, sexed-sperm-derived embryos showed reduced survival times (hazard ratio HR = 1.54, p < 0.001) which were bull dependent (p < 0.001). However, the percentage of apoptotic cells was similar to conventional embryos. Furthermore, embryos derived from sexed-sperm were found to reach developmental stages at similar timings as conventional embryos. Our results suggest that reduced conception rates after sexing are due to altered sperm morphokinetics, decreasing the chance of sperm to reach and fertilise the oocyte, and aberrant early embryonic development. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/32277124/Bovine_Sperm_Sexing_Alters_Sperm_Morphokinetics_and_Subsequent_Early_Embryonic_Development L2 - http://dx.doi.org/10.1038/s41598-020-63077-6 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.