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Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis.
Odontology. 2021 Jan; 109(1):18-28.O

Abstract

Porphyromonas gingivalis is a keystone pathogen and major colonizer in host tissue which plays a pivotal role in periodontitis among the other polymicrobial infections. Increasing facts demonstrate that curcumin has antibacterial activity and anti-biofilm effect against the periodontopathogens through diverse mechanisms that have a positive impact on periodontal health. The present study was aimed to elucidate the effect of curcumin on biofilm formation and virulence factor gene expression of P. gingivalis. By using gene expression studies, we exploited the mechanism of anti-biofilm effects of curcumin on P. gingivalis. The minimum inhibitory concentration and minimum bactericidal concentration of curcumin for both ATCC and clinical strains of P. gingivalis were found to be 62.5 and 125 µg ml-1 respectively. Curcumin prevented bacterial adhesion and biofilm formation in a dose-dependent manner. Further, curcumin attenuated the virulence of P. gingivalis by reducing the expression of genes coding for major virulence factors, including adhesions (fimA, hagA, and hagB) and proteinases (rgpA, rgpB, and kgp). The results indicated that curcumin has shown anti-biofilm as well as antibacterial activity against P. gingivalis. Further, curcumin because of its pleiotropic actions could be a simple and inexpensive therapeutic strategy in the treatment of periodontal disease.

Authors+Show Affiliations

Central Research Laboratory, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Near KSRP Ground, Bauxite road, Belgaum, Karnataka, 590 010, India.Central Research Laboratory, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Near KSRP Ground, Bauxite road, Belgaum, Karnataka, 590 010, India. Department of Pharmaceutics, Maratha Mandal's College of Pharmacy, Belgaum, Karnataka, 590 010, India.Central Research Laboratory, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Near KSRP Ground, Bauxite road, Belgaum, Karnataka, 590 010, India.Department of Biotechnology, Sinhgad College of Engineering, Affiliated to Savitribai Phule Pune University, Vadgaon Budruk, Sinhagad Road, Pune, Maharashtra, 411 0 41, India.Central Research Laboratory, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Near KSRP Ground, Bauxite road, Belgaum, Karnataka, 590 010, India.Department of Biotechnology, KLE Technological University (Formerly Known as B.V.Bhoomaraddi College of Engineering and Technology), BVB Campus, Hubli, Karnataka, 580031, India.Central Research Laboratory, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Near KSRP Ground, Bauxite road, Belgaum, Karnataka, 590 010, India. drkgbhat@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32279229

Citation

Kumbar, Vijay M., et al. "Effect of Curcumin On Growth, Biofilm Formation and Virulence Factor Gene Expression of Porphyromonas Gingivalis." Odontology, vol. 109, no. 1, 2021, pp. 18-28.
Kumbar VM, Peram MR, Kugaji MS, et al. Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis. Odontology. 2021;109(1):18-28.
Kumbar, V. M., Peram, M. R., Kugaji, M. S., Shah, T., Patil, S. P., Muddapur, U. M., & Bhat, K. G. (2021). Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis. Odontology, 109(1), 18-28. https://doi.org/10.1007/s10266-020-00514-y
Kumbar VM, et al. Effect of Curcumin On Growth, Biofilm Formation and Virulence Factor Gene Expression of Porphyromonas Gingivalis. Odontology. 2021;109(1):18-28. PubMed PMID: 32279229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis. AU - Kumbar,Vijay M, AU - Peram,Malleswara Rao, AU - Kugaji,Manohar S, AU - Shah,Tejas, AU - Patil,Sanjivani P, AU - Muddapur,Uday M, AU - Bhat,Kishore G, Y1 - 2020/04/11/ PY - 2020/01/09/received PY - 2020/03/29/accepted PY - 2020/4/13/pubmed PY - 2021/1/12/medline PY - 2020/4/13/entrez KW - Antibacterial KW - Biofilm KW - Curcumin KW - Porphyromonas gingivalis KW - RT-PCR KW - Virulence factors SP - 18 EP - 28 JF - Odontology JO - Odontology VL - 109 IS - 1 N2 - Porphyromonas gingivalis is a keystone pathogen and major colonizer in host tissue which plays a pivotal role in periodontitis among the other polymicrobial infections. Increasing facts demonstrate that curcumin has antibacterial activity and anti-biofilm effect against the periodontopathogens through diverse mechanisms that have a positive impact on periodontal health. The present study was aimed to elucidate the effect of curcumin on biofilm formation and virulence factor gene expression of P. gingivalis. By using gene expression studies, we exploited the mechanism of anti-biofilm effects of curcumin on P. gingivalis. The minimum inhibitory concentration and minimum bactericidal concentration of curcumin for both ATCC and clinical strains of P. gingivalis were found to be 62.5 and 125 µg ml-1 respectively. Curcumin prevented bacterial adhesion and biofilm formation in a dose-dependent manner. Further, curcumin attenuated the virulence of P. gingivalis by reducing the expression of genes coding for major virulence factors, including adhesions (fimA, hagA, and hagB) and proteinases (rgpA, rgpB, and kgp). The results indicated that curcumin has shown anti-biofilm as well as antibacterial activity against P. gingivalis. Further, curcumin because of its pleiotropic actions could be a simple and inexpensive therapeutic strategy in the treatment of periodontal disease. SN - 1618-1255 UR - https://www.unboundmedicine.com/medline/citation/32279229/Effect_of_curcumin_on_growth_biofilm_formation_and_virulence_factor_gene_expression_of_Porphyromonas_gingivalis_ L2 - https://dx.doi.org/10.1007/s10266-020-00514-y DB - PRIME DP - Unbound Medicine ER -