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Fumaric acid incorporated Ag/agar-agar hybrid hydrogel: A multifunctional avenue to tackle wound healing.
Mater Sci Eng C Mater Biol Appl. 2020 Jun; 111:110743.MS

Abstract

Wound and its treatment is one of the major health concerns throughout the globe. Various extrinsic and intrinsic factors can influence the dynamics of healing mechanism. One such extrinsic factor is moist environment in wound healing. The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation. But hydrated wounds often end up with patient's uncomfortability, associated infection, and tissue lipid peroxidation. Healing process prefers antimicrobial, anti-inflammatory and optimum moist microenvironment. Here, we have synthesized fumaric acid incorporated agar-silver hydrogel (AA-Ag-FA); characterized by UV-Visible spectroscopy, FTIR spectroscopy and TEM. The surface morphology is evaluated through SEM. The size of the silver nanoparticles (Ag NPs) was found to be 10-15 nm. The hydrogel shows potential antibacterial effect against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa which are predominantly responsible for wound infection. The gel shows reasonable antioxidant property evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel. Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles. The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay was found to be reasonably less when treated with AA-Ag-FA hydrogel compared to standard (Mega heal). Cytotoxicity of the samples tested through MTT assay and live-dead cell staining shows its nontoxic biocompatibility nature. In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.

Authors+Show Affiliations

GITAM Institute of Medical Sciences and Research (GIMSR), Department of Pharmacology, Visakhapatnam, Andhra Pradesh 530045, India; Department of Research and Development, Saveetha Institute of Medical and Technical Sciences (Deemed University), Thandalam, Chennai, Tamilnadu 602105, India.Indian Institute of Petroleum and Energy (IIPE), Department of Chemistry, Visakhapatnam, Andhra Pradesh 530003, India. Electronic address: somnath.chm@iipe.ac.in.VPro Biotech, 51-Arumparthapuram Main Road, Puducherry-605 110, India.GITAM Institute of Medical Sciences and Research (GIMSR), Department of Pharmacology, Visakhapatnam, Andhra Pradesh 530045, India.GITAM Institute of Medical Sciences and Research (GIMSR), Department of Microbiology, Visakhapatnam, Andhra Pradesh 530045, India.GITAM Institute of Medical Sciences and Research (GIMSR), Department of Pathology, Visakhapatnam, Andhra Pradesh 530045, India.Department of Research and Development, Saveetha Institute of Medical and Technical Sciences (Deemed University), Thandalam, Chennai, Tamilnadu 602105, India. Electronic address: drsuku3@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32279739

Citation

Basha, Syed Ilias, et al. "Fumaric Acid Incorporated Ag/agar-agar Hybrid Hydrogel: a Multifunctional Avenue to Tackle Wound Healing." Materials Science & Engineering. C, Materials for Biological Applications, vol. 111, 2020, p. 110743.
Basha SI, Ghosh S, Vinothkumar K, et al. Fumaric acid incorporated Ag/agar-agar hybrid hydrogel: A multifunctional avenue to tackle wound healing. Mater Sci Eng C Mater Biol Appl. 2020;111:110743.
Basha, S. I., Ghosh, S., Vinothkumar, K., Ramesh, B., Kumari, P. H. P., Mohan, K. V. M., & Sukumar, E. (2020). Fumaric acid incorporated Ag/agar-agar hybrid hydrogel: A multifunctional avenue to tackle wound healing. Materials Science & Engineering. C, Materials for Biological Applications, 111, 110743. https://doi.org/10.1016/j.msec.2020.110743
Basha SI, et al. Fumaric Acid Incorporated Ag/agar-agar Hybrid Hydrogel: a Multifunctional Avenue to Tackle Wound Healing. Mater Sci Eng C Mater Biol Appl. 2020;111:110743. PubMed PMID: 32279739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fumaric acid incorporated Ag/agar-agar hybrid hydrogel: A multifunctional avenue to tackle wound healing. AU - Basha,Syed Ilias, AU - Ghosh,Somnath, AU - Vinothkumar,K, AU - Ramesh,B, AU - Kumari,P Hema Praksh, AU - Mohan,K V Murali, AU - Sukumar,E, Y1 - 2020/02/13/ PY - 2019/08/24/received PY - 2020/02/11/revised PY - 2020/02/12/accepted PY - 2020/4/14/entrez PY - 2020/4/14/pubmed PY - 2021/1/5/medline KW - Agar-silver hydrogel KW - Antibacterial KW - Antioxidant KW - Collagen-growth-factor KW - Wound healing SP - 110743 EP - 110743 JF - Materials science & engineering. C, Materials for biological applications JO - Mater Sci Eng C Mater Biol Appl VL - 111 N2 - Wound and its treatment is one of the major health concerns throughout the globe. Various extrinsic and intrinsic factors can influence the dynamics of healing mechanism. One such extrinsic factor is moist environment in wound healing. The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation. But hydrated wounds often end up with patient's uncomfortability, associated infection, and tissue lipid peroxidation. Healing process prefers antimicrobial, anti-inflammatory and optimum moist microenvironment. Here, we have synthesized fumaric acid incorporated agar-silver hydrogel (AA-Ag-FA); characterized by UV-Visible spectroscopy, FTIR spectroscopy and TEM. The surface morphology is evaluated through SEM. The size of the silver nanoparticles (Ag NPs) was found to be 10-15 nm. The hydrogel shows potential antibacterial effect against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa which are predominantly responsible for wound infection. The gel shows reasonable antioxidant property evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel. Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles. The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay was found to be reasonably less when treated with AA-Ag-FA hydrogel compared to standard (Mega heal). Cytotoxicity of the samples tested through MTT assay and live-dead cell staining shows its nontoxic biocompatibility nature. In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly. SN - 1873-0191 UR - https://www.unboundmedicine.com/medline/citation/32279739/Fumaric_acid_incorporated_Ag/agar-agar_hybrid_hydrogel:_A_multifunctional_avenue_to_tackle_wound_healing. L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-4931(19)33140-6 DB - PRIME DP - Unbound Medicine ER -