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Facile coconut inflorescence sap mediated synthesis of silver nanoparticles and its diverse antimicrobial and cytotoxic properties.
Mater Sci Eng C Mater Biol Appl. 2020 Jun; 111:110834.MS

Abstract

Green synthesis of nanoparticles (NPs) involves the use of diverse extracts of biological origin as substrates to synthesize NPs and can overcome the hazards associated with chemical methods. Coconut inflorescence sap, which is unfermented phloem sap obtained by tapping of coconut inflorescence, is a rich source of sugars and secondary metabolites. In this study, coconut inflorescence sap was used to synthesize silver NPs (AgNPs). We have initially undertaken metabolomic profiling of coconut inflorescence sap from West Coast Tall cultivar to delineate its individual components. It was found to comprise of 64% secondary metabolites, 9% sugars, 12% lipids/fats and 9% peptides in positive mode, whereas in the negative mode, it was 33, 20, 9 and 11%, respectively. The concentration of silver nitrate, inflorescence sap and incubation temperature for the synthesis of AgNPs were optimized. Incubating the reaction mixture at 40 °C was found to enhance AgNP synthesis. The AgNPs synthesized were characterized using UV-visible (UV-Vis) spectrophotometry, X-Ray Diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR) and Transmission Electron Microscopy (TEM). The particles were crystalline in nature and the bulk of the particles were spherical with smooth (thin) shell and poly-dispersed with a diameter ranging from 10 nm to 30 nm. Antimicrobial property of AgNPs was tested in tissue culture of arecanut (Areca catechu L.) where bacterial contamination (Bacillus pumilus) was a frequent occurrence. A significant reduction in the contamination was observed when plantlets were treated with aqueous solutions of AgNPs. Notably, treatment with AgNPs did not affect the growth and development of the arecanut plantlets. Antimicrobial properties of AgNPs synthesized from inflorescence sap were also evaluated in human pathogenic bacteria viz., Escherichia coli ATCC 25922; Salmonella Typhimurium ATCC 14028 and Vibrio parahaemolyticus AQ4037. The antibacterial action was confirmed by determining the production of reactive oxygen species (ROS) and protein leakage studies. Cytotoxicity of AgNPs was quantified in HeLa cells. The viability (%) of HeLa cells declined significantly at 10 mg L-1 concentration of AgNP and complete mortality was observed at a concentration of 60 mg L-1. The study concludes that unfermented inflorescence sap, with above neutral pH, serves as an excellent reducing agent to synthesize AgNPs from Ag+.

Authors+Show Affiliations

ICAR-Central Plantation Crops Research Institute, Kasaragod 671 124, Kerala, India. Electronic address: rajesh.mk@icar.gov.in.ICAR-Central Plantation Crops Research Institute, Kasaragod 671 124, Kerala, India.Central University of Kerala, Kasaragod 671 316, Kerala, India.Nitte University Center for Science Education & Research, Nitte (Deemed to be University), Mangaluru 575 018, Karnataka, India.Graduate Institute of Applied Science and Technology Department, National Taiwan University of Science and Technology, Taipei 106 07, Taiwan.Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru 560 064, Karnataka, India.ICAR-National Bureau of Agricultural Insect Resources, Bengaluru 560 064, Karnataka, India.Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Mangaluru 575 018, Karnataka, India.Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Mangaluru 575 018, Karnataka, India.Central University of Kerala, Kasaragod 671 316, Kerala, India.Nitte University Center for Science Education & Research, Nitte (Deemed to be University), Mangaluru 575 018, Karnataka, India.ICAR-Central Plantation Crops Research Institute, Kasaragod 671 124, Kerala, India.ICAR-Central Plantation Crops Research Institute, Kasaragod 671 124, Kerala, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32279817

Citation

M K, Rajesh, et al. "Facile Coconut Inflorescence Sap Mediated Synthesis of Silver Nanoparticles and Its Diverse Antimicrobial and Cytotoxic Properties." Materials Science & Engineering. C, Materials for Biological Applications, vol. 111, 2020, p. 110834.
M K R, K S M, Nair SS, et al. Facile coconut inflorescence sap mediated synthesis of silver nanoparticles and its diverse antimicrobial and cytotoxic properties. Mater Sci Eng C Mater Biol Appl. 2020;111:110834.
M K, R., K S, M., Nair, S. S., B Krishna, K., T M, S., K P, S., K, S., H, S., T S Keshava, P., Neeli, C., Karunasagar, I., K B, H., & Karun, A. (2020). Facile coconut inflorescence sap mediated synthesis of silver nanoparticles and its diverse antimicrobial and cytotoxic properties. Materials Science & Engineering. C, Materials for Biological Applications, 111, 110834. https://doi.org/10.1016/j.msec.2020.110834
M K R, et al. Facile Coconut Inflorescence Sap Mediated Synthesis of Silver Nanoparticles and Its Diverse Antimicrobial and Cytotoxic Properties. Mater Sci Eng C Mater Biol Appl. 2020;111:110834. PubMed PMID: 32279817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Facile coconut inflorescence sap mediated synthesis of silver nanoparticles and its diverse antimicrobial and cytotoxic properties. AU - M K,Rajesh, AU - K S,Muralikrishna, AU - Nair,Swapna S, AU - B Krishna,Kumar, AU - T M,Subrahmanya, AU - K P,Sonu, AU - K,Subaharan, AU - H,Sweta, AU - T S Keshava,Prasad, AU - Neeli,Chandran, AU - Karunasagar,Indrani, AU - K B,Hebbar, AU - Karun,Anitha, Y1 - 2020/03/10/ PY - 2019/10/10/received PY - 2020/02/18/revised PY - 2020/03/09/accepted PY - 2020/4/14/entrez PY - 2020/4/14/pubmed PY - 2021/1/8/medline KW - Antimicrobial activity KW - Coconut inflorescence sap KW - Cytotoxicity KW - Green synthesis KW - Metabolome analysis KW - Silver nanoparticles SP - 110834 EP - 110834 JF - Materials science & engineering. C, Materials for biological applications JO - Mater Sci Eng C Mater Biol Appl VL - 111 N2 - Green synthesis of nanoparticles (NPs) involves the use of diverse extracts of biological origin as substrates to synthesize NPs and can overcome the hazards associated with chemical methods. Coconut inflorescence sap, which is unfermented phloem sap obtained by tapping of coconut inflorescence, is a rich source of sugars and secondary metabolites. In this study, coconut inflorescence sap was used to synthesize silver NPs (AgNPs). We have initially undertaken metabolomic profiling of coconut inflorescence sap from West Coast Tall cultivar to delineate its individual components. It was found to comprise of 64% secondary metabolites, 9% sugars, 12% lipids/fats and 9% peptides in positive mode, whereas in the negative mode, it was 33, 20, 9 and 11%, respectively. The concentration of silver nitrate, inflorescence sap and incubation temperature for the synthesis of AgNPs were optimized. Incubating the reaction mixture at 40 °C was found to enhance AgNP synthesis. The AgNPs synthesized were characterized using UV-visible (UV-Vis) spectrophotometry, X-Ray Diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR) and Transmission Electron Microscopy (TEM). The particles were crystalline in nature and the bulk of the particles were spherical with smooth (thin) shell and poly-dispersed with a diameter ranging from 10 nm to 30 nm. Antimicrobial property of AgNPs was tested in tissue culture of arecanut (Areca catechu L.) where bacterial contamination (Bacillus pumilus) was a frequent occurrence. A significant reduction in the contamination was observed when plantlets were treated with aqueous solutions of AgNPs. Notably, treatment with AgNPs did not affect the growth and development of the arecanut plantlets. Antimicrobial properties of AgNPs synthesized from inflorescence sap were also evaluated in human pathogenic bacteria viz., Escherichia coli ATCC 25922; Salmonella Typhimurium ATCC 14028 and Vibrio parahaemolyticus AQ4037. The antibacterial action was confirmed by determining the production of reactive oxygen species (ROS) and protein leakage studies. Cytotoxicity of AgNPs was quantified in HeLa cells. The viability (%) of HeLa cells declined significantly at 10 mg L-1 concentration of AgNP and complete mortality was observed at a concentration of 60 mg L-1. The study concludes that unfermented inflorescence sap, with above neutral pH, serves as an excellent reducing agent to synthesize AgNPs from Ag+. SN - 1873-0191 UR - https://www.unboundmedicine.com/medline/citation/32279817/Facile_coconut_inflorescence_sap_mediated_synthesis_of_silver_nanoparticles_and_its_diverse_antimicrobial_and_cytotoxic_properties_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-4931(19)33769-5 DB - PRIME DP - Unbound Medicine ER -