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Effects of Ultrasound-Assisted Extraction and Solvent on the Phenolic Profile, Bacterial Growth, and Anti-Inflammatory/Antioxidant Activities of Mediterranean Olive and Fig Leaves Extracts.
Molecules. 2020 Apr 09; 25(7)M

Abstract

Mediterranean plants, such as fig and olive leaves, are well-known to exert beneficial effects in humans because of the presence of a wide range of bioactive compounds. However, scarce information regarding the impact of extraction methods, such as ultrasound and types of solvents, on their profile of antioxidant and anti-inflammatory compounds is provided. In addition, no information is available on the effects of extraction methods and solvents on the inhibition of pathogenic bacteria or promoting probiotic growth. In this scenario, this study was aimed to study the effects of ultrasound-assisted extraction (UAE) and solvent on the phenolic profile (Triple TOF-LC-MS/MS), antioxidant and anti-inflammatory compounds of olive and fig leaves. Results showed that UAE extracted more carotenoids compared to conventional extraction, while the conventional extraction impacted on higher flavonoids (olive leaves) and total phenolics (fig leaves). The antioxidant capacity of aqueous extract of fig leaves was three times higher than the extract obtained with ethanol for conventional extraction and four times higher for UAE. In general terms, hydroethanolic extracts presented the highest bacterial growth inhibition, and showed the highest anti-inflammatory activity. In conclusion, these side streams can be used as sources of bioactive compounds for further development of high-added-value products.

Authors+Show Affiliations

Department of Biotechnology, Institute of Agrochemistry and Food Technology, Spanish National Research Council (IATA-CSIC), Av. Agustin Escardino 7, 46980 Valencia, Spain.Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. Nutrition and Food Science Area, Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Universitat de València, Avda. Vicent Andrés Estellés, 46100 València, Spain.Nutrition and Food Science Area, Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Universitat de València, Avda. Vicent Andrés Estellés, 46100 València, Spain. Range Ecology Laboratory in the Institute of Arid Regions (IRA) of Medenine, 4100 Medenine, Tunisia.Grupo de Análisis y Simulación de Procesos Agroalimentarios (ASPA), Departamento de Tecnología de Alimentos, Universitat Politècnica de València, 46022 Valencia, Spain.Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia.Centro Tecnológico de la Carne de Galicia, Adva. Galicia n° 4, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain.Department of Biotechnology, Institute of Agrochemistry and Food Technology, Spanish National Research Council (IATA-CSIC), Av. Agustin Escardino 7, 46980 Valencia, Spain.Food Processing and Quality, Production Systems Unit-Natural Resources Institute Finland (Luke)-Tietotie 2, FI-02150 Espoo, Finland.Nutrition and Food Science Area, Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Universitat de València, Avda. Vicent Andrés Estellés, 46100 València, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32283592

Citation

Alcántara, Cristina, et al. "Effects of Ultrasound-Assisted Extraction and Solvent On the Phenolic Profile, Bacterial Growth, and Anti-Inflammatory/Antioxidant Activities of Mediterranean Olive and Fig Leaves Extracts." Molecules (Basel, Switzerland), vol. 25, no. 7, 2020.
Alcántara C, Žugčić T, Abdelkebir R, et al. Effects of Ultrasound-Assisted Extraction and Solvent on the Phenolic Profile, Bacterial Growth, and Anti-Inflammatory/Antioxidant Activities of Mediterranean Olive and Fig Leaves Extracts. Molecules. 2020;25(7).
Alcántara, C., Žugčić, T., Abdelkebir, R., García-Pérez, J. V., Jambrak, A. R., Lorenzo, J. M., Collado, M. C., Granato, D., & Barba, F. J. (2020). Effects of Ultrasound-Assisted Extraction and Solvent on the Phenolic Profile, Bacterial Growth, and Anti-Inflammatory/Antioxidant Activities of Mediterranean Olive and Fig Leaves Extracts. Molecules (Basel, Switzerland), 25(7). https://doi.org/10.3390/molecules25071718
Alcántara C, et al. Effects of Ultrasound-Assisted Extraction and Solvent On the Phenolic Profile, Bacterial Growth, and Anti-Inflammatory/Antioxidant Activities of Mediterranean Olive and Fig Leaves Extracts. Molecules. 2020 Apr 9;25(7) PubMed PMID: 32283592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Ultrasound-Assisted Extraction and Solvent on the Phenolic Profile, Bacterial Growth, and Anti-Inflammatory/Antioxidant Activities of Mediterranean Olive and Fig Leaves Extracts. AU - Alcántara,Cristina, AU - Žugčić,Tihana, AU - Abdelkebir,Radhia, AU - García-Pérez,Jose V, AU - Jambrak,Anet Režek, AU - Lorenzo,José M, AU - Collado,María Carmen, AU - Granato,Daniel, AU - Barba,Francisco J, Y1 - 2020/04/09/ PY - 2020/03/02/received PY - 2020/04/07/revised PY - 2020/04/08/accepted PY - 2020/4/15/entrez PY - 2020/4/15/pubmed PY - 2021/1/7/medline KW - LC-MS KW - anti-inflammatory response KW - antioxidant methods KW - bioactive compounds KW - extraction techniques KW - reactive oxygen species JF - Molecules (Basel, Switzerland) JO - Molecules VL - 25 IS - 7 N2 - Mediterranean plants, such as fig and olive leaves, are well-known to exert beneficial effects in humans because of the presence of a wide range of bioactive compounds. However, scarce information regarding the impact of extraction methods, such as ultrasound and types of solvents, on their profile of antioxidant and anti-inflammatory compounds is provided. In addition, no information is available on the effects of extraction methods and solvents on the inhibition of pathogenic bacteria or promoting probiotic growth. In this scenario, this study was aimed to study the effects of ultrasound-assisted extraction (UAE) and solvent on the phenolic profile (Triple TOF-LC-MS/MS), antioxidant and anti-inflammatory compounds of olive and fig leaves. Results showed that UAE extracted more carotenoids compared to conventional extraction, while the conventional extraction impacted on higher flavonoids (olive leaves) and total phenolics (fig leaves). The antioxidant capacity of aqueous extract of fig leaves was three times higher than the extract obtained with ethanol for conventional extraction and four times higher for UAE. In general terms, hydroethanolic extracts presented the highest bacterial growth inhibition, and showed the highest anti-inflammatory activity. In conclusion, these side streams can be used as sources of bioactive compounds for further development of high-added-value products. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/32283592/Effects_of_Ultrasound_Assisted_Extraction_and_Solvent_on_the_Phenolic_Profile_Bacterial_Growth_and_Anti_Inflammatory/Antioxidant_Activities_of_Mediterranean_Olive_and_Fig_Leaves_Extracts_ DB - PRIME DP - Unbound Medicine ER -