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Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30.
Bioresour Bioprocess. 2018; 5(1):40.BB

Abstract

Enhancing cellulase production in Trichoderma reesei is of great interest for an economical biorefinery. Artificial transcription factors are a potentially powerful molecular strategy for improving cellulase production in T. reesei. In this study, enhanced transcriptional activators XYR1VP, ACE2VP, and ACE1VP were constructed by linking the C terminus of XYR1, ACE2, or ACE1 with an activation domain of herpes simplex virus protein VP16. T. reesei transformants TXYR1VP, TACE2VP, and TACE1VP showed improved cellulase and/or xylanase production. TXYR1VP has a cellulase-free phenotype but with significantly elevated xylanase production. Xylanase I and xylanase II activities [U/(mg biomass)] increased by 51% and 80%, respectively, in TXYR1VP in comparison with parental strain RUT C30. The filter paper activity of TACE2VP in the Avicel-based medium increased by 52% compared to that of RUT C30. In the Avicel-based medium, TACE1VP manifested an 80% increase in FPase activity and a 50% increase in xylanase activity as compared to those of RUT C30. Additionally, when pretreated corn stover was hydrolyzed, crude enzymes produced from TACE1VP yielded a greater glucose release than did the enzymes produced by parental strain RUT C30.

Authors+Show Affiliations

1New World Institute of Biotechnology, State Key Lab of Bioreactor Engineering, East China University of Science and Technology, P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China.1New World Institute of Biotechnology, State Key Lab of Bioreactor Engineering, East China University of Science and Technology, P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China.2State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237 China.1New World Institute of Biotechnology, State Key Lab of Bioreactor Engineering, East China University of Science and Technology, P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China.1New World Institute of Biotechnology, State Key Lab of Bioreactor Engineering, East China University of Science and Technology, P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32288986

Citation

Zhang, Jiajia, et al. "Construction of Enhanced Transcriptional Activators for Improving Cellulase Production in Trichoderma Reesei RUT C30." Bioresources and Bioprocessing, vol. 5, no. 1, 2018, p. 40.
Zhang J, Wu C, Wang W, et al. Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30. Bioresour Bioprocess. 2018;5(1):40.
Zhang, J., Wu, C., Wang, W., Wang, W., & Wei, D. (2018). Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30. Bioresources and Bioprocessing, 5(1), 40. https://doi.org/10.1186/s40643-018-0226-4
Zhang J, et al. Construction of Enhanced Transcriptional Activators for Improving Cellulase Production in Trichoderma Reesei RUT C30. Bioresour Bioprocess. 2018;5(1):40. PubMed PMID: 32288986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30. AU - Zhang,Jiajia, AU - Wu,Chuan, AU - Wang,Wei, AU - Wang,Wei, AU - Wei,Dongzhi, Y1 - 2018/08/18/ PY - 2018/06/09/received PY - 2018/08/14/accepted PY - 2020/4/15/entrez PY - 2018/1/1/pubmed PY - 2018/1/1/medline KW - ACE1 KW - ACE2 KW - Cellulase KW - Enhanced transcriptional activator KW - Transcription factor KW - Trichoderma reesei KW - XYR1 SP - 40 EP - 40 JF - Bioresources and bioprocessing JO - Bioresour Bioprocess VL - 5 IS - 1 N2 - Enhancing cellulase production in Trichoderma reesei is of great interest for an economical biorefinery. Artificial transcription factors are a potentially powerful molecular strategy for improving cellulase production in T. reesei. In this study, enhanced transcriptional activators XYR1VP, ACE2VP, and ACE1VP were constructed by linking the C terminus of XYR1, ACE2, or ACE1 with an activation domain of herpes simplex virus protein VP16. T. reesei transformants TXYR1VP, TACE2VP, and TACE1VP showed improved cellulase and/or xylanase production. TXYR1VP has a cellulase-free phenotype but with significantly elevated xylanase production. Xylanase I and xylanase II activities [U/(mg biomass)] increased by 51% and 80%, respectively, in TXYR1VP in comparison with parental strain RUT C30. The filter paper activity of TACE2VP in the Avicel-based medium increased by 52% compared to that of RUT C30. In the Avicel-based medium, TACE1VP manifested an 80% increase in FPase activity and a 50% increase in xylanase activity as compared to those of RUT C30. Additionally, when pretreated corn stover was hydrolyzed, crude enzymes produced from TACE1VP yielded a greater glucose release than did the enzymes produced by parental strain RUT C30. SN - 2197-4365 UR - https://www.unboundmedicine.com/medline/citation/32288986/Construction_of_enhanced_transcriptional_activators_for_improving_cellulase_production_in_Trichoderma_reesei_RUT_C30_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32288986/ DB - PRIME DP - Unbound Medicine ER -
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