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Immunization with Human Cytomegalovirus Core Fusion Machinery and Accessory Envelope Proteins Elicit Strong Synergistic Neutralizing Activities.
Vaccines (Basel). 2020 Apr 13; 8(2)V

Abstract

Human cytomegalovirus (HCMV) core fusion machinery proteins gB and gH/gL, and accessory proteins UL128/UL130/UL131A, are the key envelope proteins that mediate HCMV entry into and infection of host cells. To determine whether these HCMV envelope proteins could elicit neutralizing activities synergistically, we immunized rabbits with individual or various combinations of these proteins adsorbed to aluminum hydroxide mixed with CpG-ODN. We then analyzed serum neutralizing activities with multiple HCMV laboratory strains and clinical isolates. HCMV trimeric gB and gH/gL elicited high and moderate titers of HCMV neutralizing activity, respectively. HCMV gB in combination with gH/gL elicited up to 17-fold higher HCMV neutralizing activities compared to the sum of neutralizing activity elicited by the individual proteins analyzed with both fibroblasts and epithelial cells. HCMV gB+gH/gL+UL128/UL130/UL131A in combination increased the neutralizing activity up to 32-fold compared to the sum of neutralizing activities elicited by the individual proteins analyzed with epithelial cells. Adding UL128/UL130/UL131A to gB and gH/gL combination did not increase further the HCMV neutralizing activity analyzed with fibroblasts. These data suggest that the combination of HCMV core fusion machinery envelope proteins gB+gH/gL or the combination of gB and pentameric complex could be ideal vaccine candidates that would induce optimal immune responses against HCMV infection.

Authors+Show Affiliations

The Institute for Vaccine Research and Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.The Institute for Vaccine Research and Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.CMV Research Foundation, Richmond, VA 23229, USA.Department of Pediatrics, Virginia Commonwealth University, Richmond, VA 23298, USA.The Institute for Vaccine Research and Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32294946

Citation

Cui, Xinle, et al. "Immunization With Human Cytomegalovirus Core Fusion Machinery and Accessory Envelope Proteins Elicit Strong Synergistic Neutralizing Activities." Vaccines, vol. 8, no. 2, 2020.
Cui X, Cao Z, Wang S, et al. Immunization with Human Cytomegalovirus Core Fusion Machinery and Accessory Envelope Proteins Elicit Strong Synergistic Neutralizing Activities. Vaccines (Basel). 2020;8(2).
Cui, X., Cao, Z., Wang, S., Adler, S. P., McVoy, M. A., & Snapper, C. M. (2020). Immunization with Human Cytomegalovirus Core Fusion Machinery and Accessory Envelope Proteins Elicit Strong Synergistic Neutralizing Activities. Vaccines, 8(2). https://doi.org/10.3390/vaccines8020179
Cui X, et al. Immunization With Human Cytomegalovirus Core Fusion Machinery and Accessory Envelope Proteins Elicit Strong Synergistic Neutralizing Activities. Vaccines (Basel). 2020 Apr 13;8(2) PubMed PMID: 32294946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunization with Human Cytomegalovirus Core Fusion Machinery and Accessory Envelope Proteins Elicit Strong Synergistic Neutralizing Activities. AU - Cui,Xinle, AU - Cao,Zhouhong, AU - Wang,Shuishu, AU - Adler,Stuart P, AU - McVoy,Michael A, AU - Snapper,Clifford M, Y1 - 2020/04/13/ PY - 2020/03/13/received PY - 2020/04/04/revised PY - 2020/04/10/accepted PY - 2020/4/17/entrez PY - 2020/4/17/pubmed PY - 2020/4/17/medline KW - HCMV vaccine development KW - UL128/UL130/UL131A KW - congenital infection KW - core fusion machinery KW - envelope glycoprotein KW - gB KW - gH/gL KW - human cytomegalovirus KW - neutralizing antibody KW - solid organ and hematopoietic stem cell transplantation JF - Vaccines JO - Vaccines (Basel) VL - 8 IS - 2 N2 - Human cytomegalovirus (HCMV) core fusion machinery proteins gB and gH/gL, and accessory proteins UL128/UL130/UL131A, are the key envelope proteins that mediate HCMV entry into and infection of host cells. To determine whether these HCMV envelope proteins could elicit neutralizing activities synergistically, we immunized rabbits with individual or various combinations of these proteins adsorbed to aluminum hydroxide mixed with CpG-ODN. We then analyzed serum neutralizing activities with multiple HCMV laboratory strains and clinical isolates. HCMV trimeric gB and gH/gL elicited high and moderate titers of HCMV neutralizing activity, respectively. HCMV gB in combination with gH/gL elicited up to 17-fold higher HCMV neutralizing activities compared to the sum of neutralizing activity elicited by the individual proteins analyzed with both fibroblasts and epithelial cells. HCMV gB+gH/gL+UL128/UL130/UL131A in combination increased the neutralizing activity up to 32-fold compared to the sum of neutralizing activities elicited by the individual proteins analyzed with epithelial cells. Adding UL128/UL130/UL131A to gB and gH/gL combination did not increase further the HCMV neutralizing activity analyzed with fibroblasts. These data suggest that the combination of HCMV core fusion machinery envelope proteins gB+gH/gL or the combination of gB and pentameric complex could be ideal vaccine candidates that would induce optimal immune responses against HCMV infection. SN - 2076-393X UR - https://www.unboundmedicine.com/medline/citation/32294946/Immunization_with_Human_Cytomegalovirus_Core_Fusion_Machinery_and_Accessory_Envelope_Proteins_Elicit_Strong_Synergistic_Neutralizing_Activities_ DB - PRIME DP - Unbound Medicine ER -
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