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DNA enzyme mediated ratiometric fluorescence assay for Pb(II) ion using magnetic nanosphere-loaded gold nanoparticles and CdSe/ZnS quantum dots.
Mikrochim Acta. 2020 04 15; 187(5):273.MA

Abstract

Based on the inner filter effect mechanism of quantum dots, a ratiometric fluorescence nanoprobe was constructed for the determination of Pb(II) ion. Green emitting quantum dots conjugated with DNA substrate (DNA2) acted as donors providing green fluorescence, while gold nanoparticles coupled with DNA enzyme (DNA1) as acceptors quench the green fluorescence. Meanwhile, Fe3O4 nanosphere served as magnetic substrates to facilitate separation process and red fluorescence as an "inner rule" to eliminate the background signal. In the presence of Pb(II) ion, the DNA1 specifically recognize and capture Pb(II) ion with enhanced catalytic activity, which can cleave DNA2 and "turn on" the green fluorescence (I540), while the red fluorescence (I630) remained unchanged. In this way, the ratio of I540/I630 reflects the Pb(II) ion in the system, enabling the quantitative and selective determination of Pb(II) ion over nine different metal ions. Under optimal conditions, the ratiometric fluorescence assay showed good linearity (R2 = 0.98) within the range 10 to 100 ng mL-1. The limit of detection (LOD) was calculated to be 1.79 pg mL-1 (S/N = 3, n = 3, ±3.8%). The proposed fluorescence nanoprobe provides better sensitivity and accuracy than non-ratiometric signal evaluation for Pb(II) ion determination. Schematic representation of ratiometric fluorescence nanoprobe for Pb(II) ion detection using green fluorescence of I540 as "signal switch" and red fluorescence of I630 as "inner rule." Graphical abstract.

Authors+Show Affiliations

National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), College of Bioengineering and Food, Hubei University of Technology, Wuhan, 430068, Hubei, People's Republic of China. longquan.good@163.com.National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), College of Bioengineering and Food, Hubei University of Technology, Wuhan, 430068, Hubei, People's Republic of China.College of Life Science, Yangtze University, Jingzhou, 434023, Hubei, People's Republic of China.College of Food Science and Technology, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, People's Republic of China.College of Food Science and Technology, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, People's Republic of China.College of Food Science and Technology, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, People's Republic of China. chenyiping@mail.hzau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32296959

Citation

Wu, Long, et al. "DNA Enzyme Mediated Ratiometric Fluorescence Assay for Pb(II) Ion Using Magnetic Nanosphere-loaded Gold Nanoparticles and CdSe/ZnS Quantum Dots." Mikrochimica Acta, vol. 187, no. 5, 2020, p. 273.
Wu L, Zhu L, Ma J, et al. DNA enzyme mediated ratiometric fluorescence assay for Pb(II) ion using magnetic nanosphere-loaded gold nanoparticles and CdSe/ZnS quantum dots. Mikrochim Acta. 2020;187(5):273.
Wu, L., Zhu, L., Ma, J., Li, J., Liu, J., & Chen, Y. (2020). DNA enzyme mediated ratiometric fluorescence assay for Pb(II) ion using magnetic nanosphere-loaded gold nanoparticles and CdSe/ZnS quantum dots. Mikrochimica Acta, 187(5), 273. https://doi.org/10.1007/s00604-020-04230-w
Wu L, et al. DNA Enzyme Mediated Ratiometric Fluorescence Assay for Pb(II) Ion Using Magnetic Nanosphere-loaded Gold Nanoparticles and CdSe/ZnS Quantum Dots. Mikrochim Acta. 2020 04 15;187(5):273. PubMed PMID: 32296959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA enzyme mediated ratiometric fluorescence assay for Pb(II) ion using magnetic nanosphere-loaded gold nanoparticles and CdSe/ZnS quantum dots. AU - Wu,Long, AU - Zhu,Lin, AU - Ma,Jing, AU - Li,Jinjie, AU - Liu,Jiawei, AU - Chen,Yiping, Y1 - 2020/04/15/ PY - 2019/11/01/received PY - 2020/03/23/accepted PY - 2020/4/17/entrez PY - 2020/4/17/pubmed PY - 2020/12/2/medline KW - Fluorescence nanoprobe KW - Inner filter effect KW - Inner rule KW - Magnetic separation KW - Metal ions KW - Tea sample SP - 273 EP - 273 JF - Mikrochimica acta JO - Mikrochim Acta VL - 187 IS - 5 N2 - Based on the inner filter effect mechanism of quantum dots, a ratiometric fluorescence nanoprobe was constructed for the determination of Pb(II) ion. Green emitting quantum dots conjugated with DNA substrate (DNA2) acted as donors providing green fluorescence, while gold nanoparticles coupled with DNA enzyme (DNA1) as acceptors quench the green fluorescence. Meanwhile, Fe3O4 nanosphere served as magnetic substrates to facilitate separation process and red fluorescence as an "inner rule" to eliminate the background signal. In the presence of Pb(II) ion, the DNA1 specifically recognize and capture Pb(II) ion with enhanced catalytic activity, which can cleave DNA2 and "turn on" the green fluorescence (I540), while the red fluorescence (I630) remained unchanged. In this way, the ratio of I540/I630 reflects the Pb(II) ion in the system, enabling the quantitative and selective determination of Pb(II) ion over nine different metal ions. Under optimal conditions, the ratiometric fluorescence assay showed good linearity (R2 = 0.98) within the range 10 to 100 ng mL-1. The limit of detection (LOD) was calculated to be 1.79 pg mL-1 (S/N = 3, n = 3, ±3.8%). The proposed fluorescence nanoprobe provides better sensitivity and accuracy than non-ratiometric signal evaluation for Pb(II) ion determination. Schematic representation of ratiometric fluorescence nanoprobe for Pb(II) ion detection using green fluorescence of I540 as "signal switch" and red fluorescence of I630 as "inner rule." Graphical abstract. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/32296959/DNA_enzyme_mediated_ratiometric_fluorescence_assay_for_Pb_II__ion_using_magnetic_nanosphere_loaded_gold_nanoparticles_and_CdSe/ZnS_quantum_dots_ DB - PRIME DP - Unbound Medicine ER -