Tags

Type your tag names separated by a space and hit enter

Comparison of throat swabs and sputum specimens for viral nucleic acid detection in 52 cases of novel coronavirus (SARS-Cov-2)-infected pneumonia (COVID-19).
Clin Chem Lab Med. 2020 06 25; 58(7):1089-1094.CC

Abstract

Objectives In December 2019, a novel coronavirus (SARS-CoV-2)-infected pneumonia (COVID-19) occurred in Wuhan, China. Laboratory-based diagnostic tests utilized real-time reverse transcriptase polymerase chain reaction (RT-PCR) on throat samples. This study evaluated the diagnostic value to analyzing throat and sputum samples in order to improve accuracy and detection efficiency. Methods Paired specimens of throat swabs and sputum were obtained from 54 cases, and RNA was extracted and tested for 2019-nCoV (equated with SARS-CoV-2) by the RT-PCR assay. Results The positive rates of 2019-nCoV from sputum specimens and throat swabs were 76.9% and 44.2%, respectively. Sputum specimens showed a significantly higher positive rate than throat swabs in detecting viral nucleic acid using the RT-PCR assay (p = 0.001). Conclusions The detection rates of 2019-nCoV from sputum specimens were significantly higher than those from throat swabs. We suggest that sputum would benefit for the detection of 2019-nCoV in patients who produce sputum. The results can facilitate the selection of specimens and increase the accuracy of diagnosis.

Authors+Show Affiliations

Department of Blood Transfusion, Zhongnan Hospital of Wuhan University, Wuhan, P.R. China. Department of Medical Laboratory, Wuhan University, Wuhan, P.R. China.Department of Clinical laboratory, Wuhan Jinyintan Hospital, 1 Yintan Road, Wuhan, Hubei 430071, P.R. China.Department of Clinical laboratory, Wuhan Jinyintan Hospital, Wuhan, P.R. China.Department of Clinical laboratory, Wuhan Jinyintan Hospital, Wuhan, P.R. China.Department of Blood Transfusion, Zhongnan Hospital of Wuhan University, Wuhan, P.R. China. Department of Medical Laboratory, Wuhan University, Wuhan, P.R. China.Department of Blood Transfusion, Zhongnan Hospital of Wuhan University, Wuhan, P.R. China. Department of Medical Laboratory, Wuhan University, Wuhan, P.R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32301745

Citation

Lin, Chenyao, et al. "Comparison of Throat Swabs and Sputum Specimens for Viral Nucleic Acid Detection in 52 Cases of Novel Coronavirus (SARS-Cov-2)-infected Pneumonia (COVID-19)." Clinical Chemistry and Laboratory Medicine, vol. 58, no. 7, 2020, pp. 1089-1094.
Lin C, Xiang J, Yan M, et al. Comparison of throat swabs and sputum specimens for viral nucleic acid detection in 52 cases of novel coronavirus (SARS-Cov-2)-infected pneumonia (COVID-19). Clin Chem Lab Med. 2020;58(7):1089-1094.
Lin, C., Xiang, J., Yan, M., Li, H., Huang, S., & Shen, C. (2020). Comparison of throat swabs and sputum specimens for viral nucleic acid detection in 52 cases of novel coronavirus (SARS-Cov-2)-infected pneumonia (COVID-19). Clinical Chemistry and Laboratory Medicine, 58(7), 1089-1094. https://doi.org/10.1515/cclm-2020-0187
Lin C, et al. Comparison of Throat Swabs and Sputum Specimens for Viral Nucleic Acid Detection in 52 Cases of Novel Coronavirus (SARS-Cov-2)-infected Pneumonia (COVID-19). Clin Chem Lab Med. 2020 06 25;58(7):1089-1094. PubMed PMID: 32301745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of throat swabs and sputum specimens for viral nucleic acid detection in 52 cases of novel coronavirus (SARS-Cov-2)-infected pneumonia (COVID-19). AU - Lin,Chenyao, AU - Xiang,Jie, AU - Yan,Mingzhe, AU - Li,Hongze, AU - Huang,Shuang, AU - Shen,Changxin, PY - 2020/02/22/received PY - 2020/04/06/accepted PY - 2020/4/18/pubmed PY - 2020/6/27/medline PY - 2020/4/18/entrez KW - COVID-19 KW - SARS-Cov-2 KW - sputum specimens KW - throat swabs SP - 1089 EP - 1094 JF - Clinical chemistry and laboratory medicine JO - Clin. Chem. Lab. Med. VL - 58 IS - 7 N2 - Objectives In December 2019, a novel coronavirus (SARS-CoV-2)-infected pneumonia (COVID-19) occurred in Wuhan, China. Laboratory-based diagnostic tests utilized real-time reverse transcriptase polymerase chain reaction (RT-PCR) on throat samples. This study evaluated the diagnostic value to analyzing throat and sputum samples in order to improve accuracy and detection efficiency. Methods Paired specimens of throat swabs and sputum were obtained from 54 cases, and RNA was extracted and tested for 2019-nCoV (equated with SARS-CoV-2) by the RT-PCR assay. Results The positive rates of 2019-nCoV from sputum specimens and throat swabs were 76.9% and 44.2%, respectively. Sputum specimens showed a significantly higher positive rate than throat swabs in detecting viral nucleic acid using the RT-PCR assay (p = 0.001). Conclusions The detection rates of 2019-nCoV from sputum specimens were significantly higher than those from throat swabs. We suggest that sputum would benefit for the detection of 2019-nCoV in patients who produce sputum. The results can facilitate the selection of specimens and increase the accuracy of diagnosis. SN - 1437-4331 UR - https://www.unboundmedicine.com/medline/citation/32301745/Comparison_of_throat_swabs_and_sputum_specimens_for_viral_nucleic_acid_detection_in_52_cases_of_novel_coronavirus__SARS_Cov_2__infected_pneumonia__COVID_19__ L2 - https://www.degruyter.com/doi/10.1515/cclm-2020-0187 DB - PRIME DP - Unbound Medicine ER -