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Identifying gram-positive cocci in dermatoscopes and smartphone adapters using MALDI-TOF MS: a cross-sectional study.
An Bras Dermatol. 2020 May - Jun; 95(3):298-306.AB

Abstract

BACKGROUND

The increasingly frequent use of dermoscopy makes us think about the possibility of transfer of microorganisms, through the dermatoscope, between doctor and patients.

OBJECTIVES

To identify the most frequent gram-positive cocci in dermatoscopes and smartphone adapters, as well as the resistance profile, and to evaluate the factors associated with a higher risk of bacterial contamination of the dermatoscopes.

METHODS

A cross-sectional study was carried out with 118 dermatologists from Porto Alegre/Brazil between September 2017 and July 2018. Gram-positive cocci were identified by MALDI-TOF MS and habits of use of the dermatoscope were evaluated through an anonymous questionnaire.

RESULTS

Of the dermatoscopes analysed, 46.6% had growth of gram-positive cocci on the lens and 37.3% on the on/off button. The microorganisms most frequently found were S. epidermidis, S. hominis and S. warneri. Attending a hospital, using the dermatoscope at the hospital, with inpatients and in the intensive care unit were significantly associated with colonisation by gram-positive cocci. The highest resistance rates were observed for penicillin, erythromycin and sulfamethoxazole-trimethoprim.

STUDY LIMITATIONS

The non-search of gram-negative bacilli, fungi and viruses. Moreover, the small number of adapters did not make it possible to better define if the frequency differences were statistically significant.

CONCLUSION

Coagulase-negative staphylococci were frequently identified. S. aureus was detected only on the lens.

Authors+Show Affiliations

Gram-positive Cocci Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil; Department of Dermatology, Hospital Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, Brazil. Electronic address: mdquadros@gmail.com.Gram-positive Cocci Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.Gram-positive Cocci Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.Gram-positive Cocci Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.Gram-positive Cocci Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.Gram-positive Cocci Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32303433

Citation

Quadros, Maurício de, et al. "Identifying Gram-positive Cocci in Dermatoscopes and Smartphone Adapters Using MALDI-TOF MS: a Cross-sectional Study." Anais Brasileiros De Dermatologia, vol. 95, no. 3, 2020, pp. 298-306.
Quadros M, Bugs RCF, Soares RO, et al. Identifying gram-positive cocci in dermatoscopes and smartphone adapters using MALDI-TOF MS: a cross-sectional study. An Bras Dermatol. 2020;95(3):298-306.
Quadros, M., Bugs, R. C. F., Soares, R. O., Rossato, A. M., Rocha, L. D. L., & d'Azevedo, P. A. (2020). Identifying gram-positive cocci in dermatoscopes and smartphone adapters using MALDI-TOF MS: a cross-sectional study. Anais Brasileiros De Dermatologia, 95(3), 298-306. https://doi.org/10.1016/j.abd.2019.11.004
Quadros M, et al. Identifying Gram-positive Cocci in Dermatoscopes and Smartphone Adapters Using MALDI-TOF MS: a Cross-sectional Study. An Bras Dermatol. 2020 May - Jun;95(3):298-306. PubMed PMID: 32303433.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identifying gram-positive cocci in dermatoscopes and smartphone adapters using MALDI-TOF MS: a cross-sectional study. AU - Quadros,Maurício de, AU - Bugs,Roberto Carlos Freitas, AU - Soares,Renata de Oliveira, AU - Rossato,Adriana Medianeira, AU - Rocha,Lisiane da Luz, AU - d'Azevedo,Pedro Alves, Y1 - 2020/03/21/ PY - 2019/02/18/received PY - 2019/11/09/accepted PY - 2020/4/19/pubmed PY - 2020/6/5/medline PY - 2020/4/19/entrez KW - Dermoscopy KW - Gram-positive cocci KW - Mass spectrometry KW - Microbial sensitivity tests SP - 298 EP - 306 JF - Anais brasileiros de dermatologia JO - An Bras Dermatol VL - 95 IS - 3 N2 - BACKGROUND: The increasingly frequent use of dermoscopy makes us think about the possibility of transfer of microorganisms, through the dermatoscope, between doctor and patients. OBJECTIVES: To identify the most frequent gram-positive cocci in dermatoscopes and smartphone adapters, as well as the resistance profile, and to evaluate the factors associated with a higher risk of bacterial contamination of the dermatoscopes. METHODS: A cross-sectional study was carried out with 118 dermatologists from Porto Alegre/Brazil between September 2017 and July 2018. Gram-positive cocci were identified by MALDI-TOF MS and habits of use of the dermatoscope were evaluated through an anonymous questionnaire. RESULTS: Of the dermatoscopes analysed, 46.6% had growth of gram-positive cocci on the lens and 37.3% on the on/off button. The microorganisms most frequently found were S. epidermidis, S. hominis and S. warneri. Attending a hospital, using the dermatoscope at the hospital, with inpatients and in the intensive care unit were significantly associated with colonisation by gram-positive cocci. The highest resistance rates were observed for penicillin, erythromycin and sulfamethoxazole-trimethoprim. STUDY LIMITATIONS: The non-search of gram-negative bacilli, fungi and viruses. Moreover, the small number of adapters did not make it possible to better define if the frequency differences were statistically significant. CONCLUSION: Coagulase-negative staphylococci were frequently identified. S. aureus was detected only on the lens. SN - 1806-4841 UR - https://www.unboundmedicine.com/medline/citation/32303433/Identifying_gram-positive_cocci_in_dermatoscopes_and_smartphone_adapters_using_MALDI-TOF_MS:_a_cross-sectional_study L2 - https://linkinghub.elsevier.com/retrieve/pii/S0365-0596(20)30085-4 DB - PRIME DP - Unbound Medicine ER -