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Lipase Treatment of Dietary Krill Oil, but Not Fish Oil, Enables Enrichment of Brain Eicosapentaenoic Acid and Docosahexaenoic Acid.
Mol Nutr Food Res. 2020 06; 64(12):e2000059.MN

Abstract

SCOPE

Currently available omega-3 fatty acid supplements do not enrich the docosahexaenoic acid (DHA) of the adult brain because they are absorbed as triacylglycerol, whereas the transporter at the blood brain barrier requires lysophosphatidylcholine (LPC)-DHA. The hypothesis that treatment of krill oil (KO), which contains DHA/eicosapentaenoic acid (EPA) at the SN2 position of phosphatidylcholine, with SN1-specific lipase will generate LPC-DHA/EPA and which can be absorbed intact and transported into the brain, is tested.

METHODS

KO and fish oil (FO) are treated with Mucor meihei lipase, incorporated into AIN 93G diet, and fed to 2-month-old mice for 30 days. Fatty acid composition is analyzed by gas chromatography/mass spectroscopy. Brain derived neurotrophic factor (BDNF) is measured by ELISA.

RESULTS

Lipase-treated (LT) KO increases brain DHA and EPA, respectively, 5-and 70-fold better than untreated (UT) KO. FO, whether lipase-treated or not, has no effect on brain DHA/EPA. LTKO is also more efficient in enriching liver DHA/EPA, but less efficient than UTKO and FO in enriching adipose tissue and heart. Brain BDNF is significantly increased by LTKO, but only marginally by other preparations.

CONCLUSIONS

Pretreatment of dietary KO with lipase enables it to efficiently increase brain DHA/EPA because of the generation of LPC-DHA/EPA.

Authors+Show Affiliations

Department of Medicine, University of Illinois at Chicago, Chicago, IL, 60612, USA.Department of Medicine, University of Illinois at Chicago, Chicago, IL, 60612, USA.Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, IL, 60612, USA.Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, IL, 60612, USA.Department of Medicine, University of Illinois at Chicago, Chicago, IL, 60612, USA. Jesse Brown VA Medical Center, 820 South Damen Avenue, Chicago, IL, 60612, USA.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

32304625

Citation

Yalagala, Poorna C R., et al. "Lipase Treatment of Dietary Krill Oil, but Not Fish Oil, Enables Enrichment of Brain Eicosapentaenoic Acid and Docosahexaenoic Acid." Molecular Nutrition & Food Research, vol. 64, no. 12, 2020, pp. e2000059.
Yalagala PCR, Sugasini D, Zaldua SB, et al. Lipase Treatment of Dietary Krill Oil, but Not Fish Oil, Enables Enrichment of Brain Eicosapentaenoic Acid and Docosahexaenoic Acid. Mol Nutr Food Res. 2020;64(12):e2000059.
Yalagala, P. C. R., Sugasini, D., Zaldua, S. B., Tai, L. M., & Subbaiah, P. V. (2020). Lipase Treatment of Dietary Krill Oil, but Not Fish Oil, Enables Enrichment of Brain Eicosapentaenoic Acid and Docosahexaenoic Acid. Molecular Nutrition & Food Research, 64(12), e2000059. https://doi.org/10.1002/mnfr.202000059
Yalagala PCR, et al. Lipase Treatment of Dietary Krill Oil, but Not Fish Oil, Enables Enrichment of Brain Eicosapentaenoic Acid and Docosahexaenoic Acid. Mol Nutr Food Res. 2020;64(12):e2000059. PubMed PMID: 32304625.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipase Treatment of Dietary Krill Oil, but Not Fish Oil, Enables Enrichment of Brain Eicosapentaenoic Acid and Docosahexaenoic Acid. AU - Yalagala,Poorna C R, AU - Sugasini,Dhavamani, AU - Zaldua,Steve B, AU - Tai,Leon M, AU - Subbaiah,Papasani V, Y1 - 2020/05/04/ PY - 2020/01/21/received PY - 2020/03/31/revised PY - 2020/4/19/pubmed PY - 2021/7/13/medline PY - 2020/4/19/entrez KW - blood-brain barrier KW - fish oil KW - krill oil KW - omega 3 fatty acids SP - e2000059 EP - e2000059 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 64 IS - 12 N2 - SCOPE: Currently available omega-3 fatty acid supplements do not enrich the docosahexaenoic acid (DHA) of the adult brain because they are absorbed as triacylglycerol, whereas the transporter at the blood brain barrier requires lysophosphatidylcholine (LPC)-DHA. The hypothesis that treatment of krill oil (KO), which contains DHA/eicosapentaenoic acid (EPA) at the SN2 position of phosphatidylcholine, with SN1-specific lipase will generate LPC-DHA/EPA and which can be absorbed intact and transported into the brain, is tested. METHODS: KO and fish oil (FO) are treated with Mucor meihei lipase, incorporated into AIN 93G diet, and fed to 2-month-old mice for 30 days. Fatty acid composition is analyzed by gas chromatography/mass spectroscopy. Brain derived neurotrophic factor (BDNF) is measured by ELISA. RESULTS: Lipase-treated (LT) KO increases brain DHA and EPA, respectively, 5-and 70-fold better than untreated (UT) KO. FO, whether lipase-treated or not, has no effect on brain DHA/EPA. LTKO is also more efficient in enriching liver DHA/EPA, but less efficient than UTKO and FO in enriching adipose tissue and heart. Brain BDNF is significantly increased by LTKO, but only marginally by other preparations. CONCLUSIONS: Pretreatment of dietary KO with lipase enables it to efficiently increase brain DHA/EPA because of the generation of LPC-DHA/EPA. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/32304625/Lipase_Treatment_of_Dietary_Krill_Oil_but_Not_Fish_Oil_Enables_Enrichment_of_Brain_Eicosapentaenoic_Acid_and_Docosahexaenoic_Acid_ L2 - https://doi.org/10.1002/mnfr.202000059 DB - PRIME DP - Unbound Medicine ER -