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Silver nanoparticles synthesis using Wedelia urticifolia (Blume) DC. flower extract: Characterization and antibacterial activity evaluation.
Microsc Res Tech. 2020 Sep; 83(9):1085-1094.MR

Abstract

Silver nanoparticles (AgNPs), synthesized by green methods with the property to kill microbes, are highly valuable in medical sciences. So, the current study was aimed at using the flower extract of Wedelia urticifolia for synthesizing AgNPs with antibacterial properties. The AgNPs were produced by adding the extract to three different AgNO3 concentrations (1, 10, and 100 mM) in nine possible flower extract to metal salt ratios (9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, and 1:9). The formation of brown color and the presence of a peak at 431 nm in the UV-Vis spectrum of the colloidal solution indicates the synthesis of AgNPs, which were also characterized by dynamic light scattering (DLS), Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and energy-dispersive X-ray spectroscopy. The DLS results exposed that the smallest sized AgNPs were obtained in 10 mM AgNO3 solution and 4E6M was the optimized extract to metal salt solution ratio. The characterization techniques revealed that the synthesized AgNPs were spherical shaped and crystalline with a diameter of less than 30 nm. Furthermore, the synthesized nanoparticles were tested against two Gram-positive (Klebsiella pneumonia and Staphylococcus aureus) and two Gram-negative (Pseudomonas aeruginosa and Escherichia coli) bacterial strains for their antibacterial efficiency. Although the studied strains showed limited growth, overall, the effect of nanoparticles was found to be insignificant. It is concluded that the current study is advantageous over other previous studies because the AgNPs were synthesized at room temperature from 10 mM AgNO3 concentration in only 2 hours. Additionally, the present work is simple, ecofriendly, and in-expensive.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Rather MY
Department of Ecology and Environmental Sciences, Pondicherry University, Puducherry, India.
Shincy M
Department of Ecology and Environmental Sciences, Pondicherry University, Puducherry, India.
Sundarapandian S
Department of Ecology and Environmental Sciences, Pondicherry University, Puducherry, India.

MeSH

Anti-Bacterial AgentsBacteriaFlowersGreen Chemistry TechnologyMetal NanoparticlesMicrobial Sensitivity TestsMicroscopy, Electron, ScanningMicroscopy, Electron, TransmissionParticle SizePlant ExtractsSilverSpectroscopy, Fourier Transform InfraredWedeliaX-Ray Diffraction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32306505

Citation

Rather, Mohd Yousuf, et al. "Silver Nanoparticles Synthesis Using Wedelia Urticifolia (Blume) DC. Flower Extract: Characterization and Antibacterial Activity Evaluation." Microscopy Research and Technique, vol. 83, no. 9, 2020, pp. 1085-1094.
Rather MY, Shincy M, Sundarapandian S. Silver nanoparticles synthesis using Wedelia urticifolia (Blume) DC. flower extract: Characterization and antibacterial activity evaluation. Microsc Res Tech. 2020;83(9):1085-1094.
Rather, M. Y., Shincy, M., & Sundarapandian, S. (2020). Silver nanoparticles synthesis using Wedelia urticifolia (Blume) DC. flower extract: Characterization and antibacterial activity evaluation. Microscopy Research and Technique, 83(9), 1085-1094. https://doi.org/10.1002/jemt.23499
Rather MY, Shincy M, Sundarapandian S. Silver Nanoparticles Synthesis Using Wedelia Urticifolia (Blume) DC. Flower Extract: Characterization and Antibacterial Activity Evaluation. Microsc Res Tech. 2020;83(9):1085-1094. PubMed PMID: 32306505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver nanoparticles synthesis using Wedelia urticifolia (Blume) DC. flower extract: Characterization and antibacterial activity evaluation. AU - Rather,Mohd Yousuf, AU - Shincy,Maroli, AU - Sundarapandian,Somaiah, Y1 - 2020/04/18/ PY - 2019/12/2/received PY - 2020/2/11/revised PY - 2020/4/6/accepted PY - 2020/4/20/pubmed PY - 2021/5/20/medline PY - 2020/4/20/entrez KW - Wedelia urticifolia KW - antibacterial activity KW - biosynthesis KW - plant extract KW - silver nanoparticles SP - 1085 EP - 1094 JF - Microscopy research and technique JO - Microsc Res Tech VL - 83 IS - 9 N2 - Silver nanoparticles (AgNPs), synthesized by green methods with the property to kill microbes, are highly valuable in medical sciences. So, the current study was aimed at using the flower extract of Wedelia urticifolia for synthesizing AgNPs with antibacterial properties. The AgNPs were produced by adding the extract to three different AgNO3 concentrations (1, 10, and 100 mM) in nine possible flower extract to metal salt ratios (9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, and 1:9). The formation of brown color and the presence of a peak at 431 nm in the UV-Vis spectrum of the colloidal solution indicates the synthesis of AgNPs, which were also characterized by dynamic light scattering (DLS), Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and energy-dispersive X-ray spectroscopy. The DLS results exposed that the smallest sized AgNPs were obtained in 10 mM AgNO3 solution and 4E6M was the optimized extract to metal salt solution ratio. The characterization techniques revealed that the synthesized AgNPs were spherical shaped and crystalline with a diameter of less than 30 nm. Furthermore, the synthesized nanoparticles were tested against two Gram-positive (Klebsiella pneumonia and Staphylococcus aureus) and two Gram-negative (Pseudomonas aeruginosa and Escherichia coli) bacterial strains for their antibacterial efficiency. Although the studied strains showed limited growth, overall, the effect of nanoparticles was found to be insignificant. It is concluded that the current study is advantageous over other previous studies because the AgNPs were synthesized at room temperature from 10 mM AgNO3 concentration in only 2 hours. Additionally, the present work is simple, ecofriendly, and in-expensive. SN - 1097-0029 UR - https://www.unboundmedicine.com/medline/citation/32306505/Silver_nanoparticles_synthesis_using_Wedelia_urticifolia__Blume__DC__flower_extract:_Characterization_and_antibacterial_activity_evaluation_ DB - PRIME DP - Unbound Medicine ER -