Citation
Baek, Yun Hee, et al. "Development of a Reverse Transcription-loop-mediated Isothermal Amplification as a Rapid Early-detection Method for Novel SARS-CoV-2." Emerging Microbes & Infections, vol. 9, no. 1, 2020, pp. 998-1007.
Baek YH, Um J, Antigua KJC, et al. Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2. Emerg Microbes Infect. 2020;9(1):998-1007.
Baek, Y. H., Um, J., Antigua, K. J. C., Park, J. H., Kim, Y., Oh, S., Kim, Y. I., Choi, W. S., Kim, S. G., Jeong, J. H., Chin, B. S., Nicolas, H. D. G., Ahn, J. Y., Shin, K. S., Choi, Y. K., Park, J. S., & Song, M. S. (2020). Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2. Emerging Microbes & Infections, 9(1), 998-1007. https://doi.org/10.1080/22221751.2020.1756698
Baek YH, et al. Development of a Reverse Transcription-loop-mediated Isothermal Amplification as a Rapid Early-detection Method for Novel SARS-CoV-2. Emerg Microbes Infect. 2020;9(1):998-1007. PubMed PMID: 32306853.
TY - JOUR
T1 - Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2.
AU - Baek,Yun Hee,
AU - Um,Jihye,
AU - Antigua,Khristine Joy C,
AU - Park,Ji-Hyun,
AU - Kim,Yeonjae,
AU - Oh,Sol,
AU - Kim,Young-Il,
AU - Choi,Won-Suk,
AU - Kim,Seong Gyu,
AU - Jeong,Ju Hwan,
AU - Chin,Bum Sik,
AU - Nicolas,Halcyon Dawn G,
AU - Ahn,Ji-Young,
AU - Shin,Kyeong Seob,
AU - Choi,Young Ki,
AU - Park,Jun-Sun,
AU - Song,Min-Suk,
PY - 2020/4/21/pubmed
PY - 2020/5/20/medline
PY - 2020/4/21/entrez
KW - COVID-19
KW - SARS-CoV-2
KW - colorimetric detection
KW - molecular diagnosis
KW - novel coronavirus
KW - reverse transcription-loop-mediated isothermal amplification
SP - 998
EP - 1007
JF - Emerging microbes & infections
JO - Emerg Microbes Infect
VL - 9
IS - 1
N2 - The previous outbreaks of SARS-CoV and MERS-CoV have led researchers to study the role of diagnostics in impediment of further spread and transmission. With the recent emergence of the novel SARS-CoV-2, the availability of rapid, sensitive, and reliable diagnostic methods is essential for disease control. Hence, we have developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the specific detection of SARS-CoV-2. The primer sets for RT-LAMP assay were designed to target the nucleocapsid gene of the viral RNA, and displayed a detection limit of 102 RNA copies close to that of qRT-PCR. Notably, the assay has exhibited a rapid detection span of 30 min combined with the colorimetric visualization. This test can detect specifically viral RNAs of the SARS-CoV-2 with no cross-reactivity to related coronaviruses, such as HCoV-229E, HCoV-NL63, HCoV-OC43, and MERS-CoV as well as human infectious influenza viruses (type B, H1N1pdm, H3N2, H5N1, H5N6, H5N8, and H7N9), and other respiratory disease-causing viruses (RSVA, RSVB, ADV, PIV, MPV, and HRV). Furthermore, the developed RT-LAMP assay has been evaluated using specimens collected from COVID-19 patients that exhibited high agreement to the qRT-PCR. Our RT-LAMP assay is simple to perform, less expensive, time-efficient, and can be used in clinical laboratories for preliminary detection of SARS-CoV-2 in suspected patients. In addition to the high sensitivity and specificity, this isothermal amplification conjugated with a single-tube colorimetric detection method may contribute to the public health responses and disease control, especially in the areas with limited laboratory capacities.
SN - 2222-1751
UR - https://www.unboundmedicine.com/medline/citation/32306853/Development_of_a_reverse_transcription_loop_mediated_isothermal_amplification_as_a_rapid_early_detection_method_for_novel_SARS_CoV_2_
L2 - https://www.tandfonline.com/doi/full/10.1080/22221751.2020.1756698
DB - PRIME
DP - Unbound Medicine
ER -