TY - JOUR T1 - Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2. AU - Baek,Yun Hee, AU - Um,Jihye, AU - Antigua,Khristine Joy C, AU - Park,Ji-Hyun, AU - Kim,Yeonjae, AU - Oh,Sol, AU - Kim,Young-Il, AU - Choi,Won-Suk, AU - Kim,Seong Gyu, AU - Jeong,Ju Hwan, AU - Chin,Bum Sik, AU - Nicolas,Halcyon Dawn G, AU - Ahn,Ji-Young, AU - Shin,Kyeong Seob, AU - Choi,Young Ki, AU - Park,Jun-Sun, AU - Song,Min-Suk, PY - 2020/4/21/pubmed PY - 2020/5/20/medline PY - 2020/4/21/entrez KW - COVID-19 KW - SARS-CoV-2 KW - colorimetric detection KW - molecular diagnosis KW - novel coronavirus KW - reverse transcription-loop-mediated isothermal amplification SP - 998 EP - 1007 JF - Emerging microbes & infections JO - Emerg Microbes Infect VL - 9 IS - 1 N2 - The previous outbreaks of SARS-CoV and MERS-CoV have led researchers to study the role of diagnostics in impediment of further spread and transmission. With the recent emergence of the novel SARS-CoV-2, the availability of rapid, sensitive, and reliable diagnostic methods is essential for disease control. Hence, we have developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the specific detection of SARS-CoV-2. The primer sets for RT-LAMP assay were designed to target the nucleocapsid gene of the viral RNA, and displayed a detection limit of 102 RNA copies close to that of qRT-PCR. Notably, the assay has exhibited a rapid detection span of 30 min combined with the colorimetric visualization. This test can detect specifically viral RNAs of the SARS-CoV-2 with no cross-reactivity to related coronaviruses, such as HCoV-229E, HCoV-NL63, HCoV-OC43, and MERS-CoV as well as human infectious influenza viruses (type B, H1N1pdm, H3N2, H5N1, H5N6, H5N8, and H7N9), and other respiratory disease-causing viruses (RSVA, RSVB, ADV, PIV, MPV, and HRV). Furthermore, the developed RT-LAMP assay has been evaluated using specimens collected from COVID-19 patients that exhibited high agreement to the qRT-PCR. Our RT-LAMP assay is simple to perform, less expensive, time-efficient, and can be used in clinical laboratories for preliminary detection of SARS-CoV-2 in suspected patients. In addition to the high sensitivity and specificity, this isothermal amplification conjugated with a single-tube colorimetric detection method may contribute to the public health responses and disease control, especially in the areas with limited laboratory capacities. SN - 2222-1751 UR - https://www.unboundmedicine.com/medline/citation/32306853/Development_of_a_reverse_transcription_loop_mediated_isothermal_amplification_as_a_rapid_early_detection_method_for_novel_SARS_CoV_2_ L2 - https://www.tandfonline.com/doi/full/10.1080/22221751.2020.1756698 DB - PRIME DP - Unbound Medicine ER -