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SARS-CoV-2 RNA detection of hospital isolation wards hygiene monitoring during the Coronavirus Disease 2019 outbreak in a Chinese hospital.
Int J Infect Dis. 2020 May; 94:103-106.IJ

Abstract

OBJECTIVES

The aim of this paper was to monitor the presence of SARS-Cov-2 among hospital environment surfaces, sewage, and personal protective equipment (PPE) of staffs in isolation wards in the First Affiliated Hospital of Zhejiang University, China.

METHODS

Surfaces of objects were routinely wiped with 1000mg/L chlorine containing disinfectant. Air and sewage disinfection was proceeded routinely and strictly. Hospital environmental surfaces and PPE of staffs in isolation wards were sampled using swabs. The sewage from various inlet and outlets were sampled. The respiratory and stool specimens of patients were collected. The respiratory specimens of staffs in the isolation wards were also sampled once a week. Quantitative real-time reverse transcription PCR (qRT-PCR) methods were used to confirm the existence of SARS-Cov-2 RNA. Viral culture was done for the samples positive for SARS-Cov-2 RNA.

RESULTS

During the study period, 33 laboratory-confirmed patients were hospitalized in isolation wards in the hospital. None of SARS-Cov-2 RNA was detected among the 36 objects surface samples and 9 staffs PPE samples in isolation wards. Though the 3 sewage samples from the inlet of preprocessing disinfection pool were positive for SARS-CoV-2 RNA and the sample from the outlet of preprocessing disinfection pool was weakly positive, the sewage sample from the outlet of the last disinfection pool was negative. All of the 5 sewage samples from various points were negative by viral culture of SARS-Cov-2. None of the respiratory specimens of staffs in the isolation wards were positive.

CONCLUSIONS

Though SARS-Cov-2 RNA of the sewage samples were positive from inlets of the sewage disinfection pool and negative from the outlet of the last sewage disinfection pool, no viable virus was detected by culture. The monitoring data in this study suggested that the strict disinfection and hand hygiene could decrease the hospital-associated COVID-19 infection risk of the staffs in isolation wards.

Authors+Show Affiliations

Respiratory Department, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.Infection Control Department, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.Infection Control Department, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.Infection Control Department, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.Infection Control Department, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.General Affairs Department, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.Infection Control Department, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. Electronic address: qutingting@zju.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32311449

Citation

Wang, Jie, et al. "SARS-CoV-2 RNA Detection of Hospital Isolation Wards Hygiene Monitoring During the Coronavirus Disease 2019 Outbreak in a Chinese Hospital." International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, vol. 94, 2020, pp. 103-106.
Wang J, Feng H, Zhang S, et al. SARS-CoV-2 RNA detection of hospital isolation wards hygiene monitoring during the Coronavirus Disease 2019 outbreak in a Chinese hospital. Int J Infect Dis. 2020;94:103-106.
Wang, J., Feng, H., Zhang, S., Ni, Z., Ni, L., Chen, Y., Zhuo, L., Zhong, Z., & Qu, T. (2020). SARS-CoV-2 RNA detection of hospital isolation wards hygiene monitoring during the Coronavirus Disease 2019 outbreak in a Chinese hospital. International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, 94, 103-106. https://doi.org/10.1016/j.ijid.2020.04.024
Wang J, et al. SARS-CoV-2 RNA Detection of Hospital Isolation Wards Hygiene Monitoring During the Coronavirus Disease 2019 Outbreak in a Chinese Hospital. Int J Infect Dis. 2020;94:103-106. PubMed PMID: 32311449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SARS-CoV-2 RNA detection of hospital isolation wards hygiene monitoring during the Coronavirus Disease 2019 outbreak in a Chinese hospital. AU - Wang,Jie, AU - Feng,Haiting, AU - Zhang,Sheng, AU - Ni,Zuowei, AU - Ni,Lingmei, AU - Chen,Yu, AU - Zhuo,Lixin, AU - Zhong,Zifeng, AU - Qu,Tingting, Y1 - 2020/04/18/ PY - 2020/03/21/received PY - 2020/04/08/revised PY - 2020/04/09/accepted PY - 2020/4/21/pubmed PY - 2020/5/21/medline PY - 2020/4/21/entrez KW - COVID-19 KW - Disinfection KW - Environmental contamination KW - Healthcare-associated infection KW - SARS-CoV-2 SP - 103 EP - 106 JF - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JO - Int J Infect Dis VL - 94 N2 - OBJECTIVES: The aim of this paper was to monitor the presence of SARS-Cov-2 among hospital environment surfaces, sewage, and personal protective equipment (PPE) of staffs in isolation wards in the First Affiliated Hospital of Zhejiang University, China. METHODS: Surfaces of objects were routinely wiped with 1000mg/L chlorine containing disinfectant. Air and sewage disinfection was proceeded routinely and strictly. Hospital environmental surfaces and PPE of staffs in isolation wards were sampled using swabs. The sewage from various inlet and outlets were sampled. The respiratory and stool specimens of patients were collected. The respiratory specimens of staffs in the isolation wards were also sampled once a week. Quantitative real-time reverse transcription PCR (qRT-PCR) methods were used to confirm the existence of SARS-Cov-2 RNA. Viral culture was done for the samples positive for SARS-Cov-2 RNA. RESULTS: During the study period, 33 laboratory-confirmed patients were hospitalized in isolation wards in the hospital. None of SARS-Cov-2 RNA was detected among the 36 objects surface samples and 9 staffs PPE samples in isolation wards. Though the 3 sewage samples from the inlet of preprocessing disinfection pool were positive for SARS-CoV-2 RNA and the sample from the outlet of preprocessing disinfection pool was weakly positive, the sewage sample from the outlet of the last disinfection pool was negative. All of the 5 sewage samples from various points were negative by viral culture of SARS-Cov-2. None of the respiratory specimens of staffs in the isolation wards were positive. CONCLUSIONS: Though SARS-Cov-2 RNA of the sewage samples were positive from inlets of the sewage disinfection pool and negative from the outlet of the last sewage disinfection pool, no viable virus was detected by culture. The monitoring data in this study suggested that the strict disinfection and hand hygiene could decrease the hospital-associated COVID-19 infection risk of the staffs in isolation wards. SN - 1878-3511 UR - https://www.unboundmedicine.com/medline/citation/32311449/SARS_CoV_2_RNA_detection_of_hospital_isolation_wards_hygiene_monitoring_during_the_Coronavirus_Disease_2019_outbreak_in_a_Chinese_hospital_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1201-9712(20)30237-X DB - PRIME DP - Unbound Medicine ER -