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[CXCR2 participates in cerebral endothelial activation and neutrophil migration in mice with septic encephalopathy].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2020 Jan; 36(1):20-25.XB

Abstract

Objective To investigate the role of CXCR2 in the cerebral endothelial activation and migration of neutrophils into the brain in septic encephalopathy (SE) induced by lipopolysaccharide (LPS). Methods C57BL/6J mice and CXCR2-knockout mice were randomly divided into a normal control group, a wildtype mice group with LPS treatment and CXCR2-knockout mice group with LPS treatment. Mouse SE models were induced by intraperitoneal LPS injection. Naphthol AS-D chloroacetate histochemical staining of the brain section was performed to quantitate the neutrophils infiltrating into the cerebral cortex. TNF-α and CXCL1 concentrations in the brain and plasma were determined by ELISA. After the stimulation of LPS (1 μg/mL) and TNF-α (200 ng/mL), the levels of CXCR2 protein in the primary mouse brain microvascular endothelial cells isolated from the cerebral cortex were detected by Western blotting. The levels of F-actin and vascular cell adhesion molecule 1 (VCAM-1) protein in the primary mouse brain microvascular endothelial cells stimulated by CXCL1 (100 ng/mL) were detected by Western blotting. Results After intraperitoneal LPS injection, there was a significant increase in the level of TNF-α in the brain and plasma and there was also an evident increase in the level of CXCL1 in the brain of wild type mice (C57BL/6J mice). And intraperitoneal LPS injection caused increased neutrophil infiltration into the cerebral cortex in the wild type mice (C57BL/6J mice). But CXCR2-knockout mice displayed evidently reduced neutrophil infiltration into the cerebral cortex compared with the wildtype mice. In vitro LPS and TNF-α upregulated the expression of CXCR2 in the primary brain microvascular endothelial cells. CXCL1 increased remarkably the expression of endothelial F-actin and VCAM-1. Conclusion In the SE model, CXCR2 participates in the cerebral endothelial activation and neutrophil migration into the brain.

Authors+Show Affiliations

Department of Immunology, Bengbu Medical College, Bengbu 233030, China.Department of Immunology, Bengbu Medical College, Bengbu 233030, China.Department of Immunology, Bengbu Medical College, Bengbu 233030, China.Department of Immunology, Bengbu Medical College, Bengbu 233030, China.Department of Immunology, Bengbu Medical College, Bengbu 233030, China.Department of Immunology, Bengbu Medical College, Bengbu 233030, China. *Corresponding authors, E-mail: chuanwangsong@163.com.Department of Immunology, Bengbu Medical College, Bengbu 233030, China. *Corresponding authors, E-mail: qzq7778@hotmail.com.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

32314720

Citation

Wu, Fengjiao, et al. "[CXCR2 Participates in Cerebral Endothelial Activation and Neutrophil Migration in Mice With Septic Encephalopathy]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 36, no. 1, 2020, pp. 20-25.
Wu F, Mu M, Chen X, et al. [CXCR2 participates in cerebral endothelial activation and neutrophil migration in mice with septic encephalopathy]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2020;36(1):20-25.
Wu, F., Mu, M., Chen, X., Zhai, L., Zhang, J., Song, C., & Qian, Z. (2020). [CXCR2 participates in cerebral endothelial activation and neutrophil migration in mice with septic encephalopathy]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 36(1), 20-25.
Wu F, et al. [CXCR2 Participates in Cerebral Endothelial Activation and Neutrophil Migration in Mice With Septic Encephalopathy]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2020;36(1):20-25. PubMed PMID: 32314720.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [CXCR2 participates in cerebral endothelial activation and neutrophil migration in mice with septic encephalopathy]. AU - Wu,Fengjiao, AU - Mu,Mimi, AU - Chen,Xiaofen, AU - Zhai,Liqian, AU - Zhang,Jin, AU - Song,Chuanwang, AU - Qian,Zhongqing, PY - 2020/4/22/entrez PY - 2020/4/22/pubmed PY - 2020/6/27/medline SP - 20 EP - 25 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 36 IS - 1 N2 - Objective To investigate the role of CXCR2 in the cerebral endothelial activation and migration of neutrophils into the brain in septic encephalopathy (SE) induced by lipopolysaccharide (LPS). Methods C57BL/6J mice and CXCR2-knockout mice were randomly divided into a normal control group, a wildtype mice group with LPS treatment and CXCR2-knockout mice group with LPS treatment. Mouse SE models were induced by intraperitoneal LPS injection. Naphthol AS-D chloroacetate histochemical staining of the brain section was performed to quantitate the neutrophils infiltrating into the cerebral cortex. TNF-α and CXCL1 concentrations in the brain and plasma were determined by ELISA. After the stimulation of LPS (1 μg/mL) and TNF-α (200 ng/mL), the levels of CXCR2 protein in the primary mouse brain microvascular endothelial cells isolated from the cerebral cortex were detected by Western blotting. The levels of F-actin and vascular cell adhesion molecule 1 (VCAM-1) protein in the primary mouse brain microvascular endothelial cells stimulated by CXCL1 (100 ng/mL) were detected by Western blotting. Results After intraperitoneal LPS injection, there was a significant increase in the level of TNF-α in the brain and plasma and there was also an evident increase in the level of CXCL1 in the brain of wild type mice (C57BL/6J mice). And intraperitoneal LPS injection caused increased neutrophil infiltration into the cerebral cortex in the wild type mice (C57BL/6J mice). But CXCR2-knockout mice displayed evidently reduced neutrophil infiltration into the cerebral cortex compared with the wildtype mice. In vitro LPS and TNF-α upregulated the expression of CXCR2 in the primary brain microvascular endothelial cells. CXCL1 increased remarkably the expression of endothelial F-actin and VCAM-1. Conclusion In the SE model, CXCR2 participates in the cerebral endothelial activation and neutrophil migration into the brain. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/32314720/[CXCR2_participates_in_cerebral_endothelial_activation_and_neutrophil_migration_in_mice_with_septic_encephalopathy]_ L2 - https://medlineplus.gov/sepsis.html DB - PRIME DP - Unbound Medicine ER -