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Paeonol protects against testicular ischaemia-reperfusion injury in rats through inhibition of oxidative stress and inflammation.
Andrologia. 2020 Jul; 52(6):e13599.A

Abstract

Ischaemia-reperfusion (IR) is the most common form of testicular injury that results in oxidative damage and inflammation ending by subinfertility. Paeonol, a natural phenolic compound, exhibits antioxidant and anti-inflammatory effects. Thus, the present study investigated the role of paeonol in rat testicular IR injury. Thirty adult Wistar rats were randomly divided into five groups; sham, sham treated with paeonol, IR injury, and IR pre-treated with paeonol at low and high doses. Serum testosterone and testicular levels of malondialdehyde and reduced glutathione (GSH) besides superoxide dismutase (SOD) activity were determined. Gene quantifications for tumour necrosis factor-α (TNF-α), hypoxia-inducible factor-1α (HIF-1α) and heat shock protein 70 (HSP70) were also assessed. Histopathological pictures and the immunohistochemical expression of testicular nuclear factor erythroid 2-related factor 2 (Nrf2), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were shown. Pre-treatment with paeonol prevented the drop in serum testosterone, alongside with improvement of testicular malondialdehyde and GSH levels plus SOD activity. Paeonol regained the normal spermatogenesis with prevention of IR-induced increase in TNF-α, HIF-1α and HSP70 gene expression besides IL-1β and IL-6 immunostaining and reduction in Nrf2 protein expression. Paeonol exerted a dose-dependent beneficial effect on testicular IR injury. This effect was achieved by its antioxidant and anti-inflammatory effects.

Authors+Show Affiliations

Department of Pharmacology, Faculty of Medicine, Minia University, El-Minia, Egypt.Department of Pharmacology, Faculty of Medicine, Minia University, El-Minia, Egypt. Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, Saudi Arabia.Department of Histology, Faculty of Medicine, Minia University, El-Minia, Egypt.Department of Pharmacology, Faculty of Medicine, Minia University, El-Minia, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32314822

Citation

Mohamed, Mervat Z., et al. "Paeonol Protects Against Testicular Ischaemia-reperfusion Injury in Rats Through Inhibition of Oxidative Stress and Inflammation." Andrologia, vol. 52, no. 6, 2020, pp. e13599.
Mohamed MZ, Morsy MA, Mohamed HH, et al. Paeonol protects against testicular ischaemia-reperfusion injury in rats through inhibition of oxidative stress and inflammation. Andrologia. 2020;52(6):e13599.
Mohamed, M. Z., Morsy, M. A., Mohamed, H. H., & Hafez, H. M. (2020). Paeonol protects against testicular ischaemia-reperfusion injury in rats through inhibition of oxidative stress and inflammation. Andrologia, 52(6), e13599. https://doi.org/10.1111/and.13599
Mohamed MZ, et al. Paeonol Protects Against Testicular Ischaemia-reperfusion Injury in Rats Through Inhibition of Oxidative Stress and Inflammation. Andrologia. 2020;52(6):e13599. PubMed PMID: 32314822.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Paeonol protects against testicular ischaemia-reperfusion injury in rats through inhibition of oxidative stress and inflammation. AU - Mohamed,Mervat Z, AU - Morsy,Mohamed A, AU - Mohamed,Hanaa H, AU - Hafez,Heba M, Y1 - 2020/04/21/ PY - 2019/12/10/received PY - 2020/03/05/revised PY - 2020/03/23/accepted PY - 2020/4/22/pubmed PY - 2021/4/2/medline PY - 2020/4/22/entrez KW - HIF-1α KW - HSP70 KW - Nrf2 KW - TNF-α KW - paeonol KW - testicular ischaemia-reperfusion SP - e13599 EP - e13599 JF - Andrologia JO - Andrologia VL - 52 IS - 6 N2 - Ischaemia-reperfusion (IR) is the most common form of testicular injury that results in oxidative damage and inflammation ending by subinfertility. Paeonol, a natural phenolic compound, exhibits antioxidant and anti-inflammatory effects. Thus, the present study investigated the role of paeonol in rat testicular IR injury. Thirty adult Wistar rats were randomly divided into five groups; sham, sham treated with paeonol, IR injury, and IR pre-treated with paeonol at low and high doses. Serum testosterone and testicular levels of malondialdehyde and reduced glutathione (GSH) besides superoxide dismutase (SOD) activity were determined. Gene quantifications for tumour necrosis factor-α (TNF-α), hypoxia-inducible factor-1α (HIF-1α) and heat shock protein 70 (HSP70) were also assessed. Histopathological pictures and the immunohistochemical expression of testicular nuclear factor erythroid 2-related factor 2 (Nrf2), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were shown. Pre-treatment with paeonol prevented the drop in serum testosterone, alongside with improvement of testicular malondialdehyde and GSH levels plus SOD activity. Paeonol regained the normal spermatogenesis with prevention of IR-induced increase in TNF-α, HIF-1α and HSP70 gene expression besides IL-1β and IL-6 immunostaining and reduction in Nrf2 protein expression. Paeonol exerted a dose-dependent beneficial effect on testicular IR injury. This effect was achieved by its antioxidant and anti-inflammatory effects. SN - 1439-0272 UR - https://www.unboundmedicine.com/medline/citation/32314822/Paeonol_protects_against_testicular_ischaemia_reperfusion_injury_in_rats_through_inhibition_of_oxidative_stress_and_inflammation_ L2 - https://doi.org/10.1111/and.13599 DB - PRIME DP - Unbound Medicine ER -