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Spectrofluorimetric approach for determination of citicoline in the presence of co-formulated piracetam through fluorescence quenching of eosin Y.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Aug 05; 236:118337.SA

Abstract

A simple, sensitive, and precise spectrofluorimetric method has been developed and validated for quantitation of citicoline in its pharmaceutical formulations. The proposed method based on quantitative quenching effect of citicoline on the native fluorescence of Eosin Y via developing of a binary complex reaction between the cited drug and Eosin Y in acidic medium using acetate buffer pH = 3.6. The quenching of the fluorescence of eosin was measured at 540 nm after excitation at 518 nm. Calibration graph was achieved in the range of 300-3000 ng/mL with 0.9996 as correlation coefficient and 291.0 and 93.86 ng/mL as quantitation and detection limits, respectively. The developed method considered as the first developed spectrofluorimetric one for quantitation of citicoline with high sensitivity and validated according to ICH guidelines. The selectivity of the proposed method was investigated by studying the interference of piracetam as co-formulated drug with CIT in pharmaceutical formulation, therefore the developed method could be used for routine quality control of citicoline in its pharmaceutical formulations either alone or in combination with piracetam.

Authors+Show Affiliations

Pharmacognosy and Pharmaceutical Chemistry Department, College of Pharmacy, Taibah University, Medinah, Saudi Arabia; Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia, Egypt.Pharmaceutical Chemistry, Faculty of Pharmacy, Nahda University, 62514 Beni-Suef, Egypt. Electronic address: amal.badawy@nub.edu.eg.Pharmaceutical Chemistry, Faculty of Pharmacy, Nahda University, 62514 Beni-Suef, Egypt; Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, 62514 Beni-Suef, Egypt.Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, 62514 Beni-Suef, Egypt.Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32320917

Citation

Omar, Mahmoud A., et al. "Spectrofluorimetric Approach for Determination of Citicoline in the Presence of Co-formulated Piracetam Through Fluorescence Quenching of Eosin Y." Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, vol. 236, 2020, p. 118337.
Omar MA, Ahmed AB, Abdelwahab NS, et al. Spectrofluorimetric approach for determination of citicoline in the presence of co-formulated piracetam through fluorescence quenching of eosin Y. Spectrochim Acta A Mol Biomol Spectrosc. 2020;236:118337.
Omar, M. A., Ahmed, A. B., Abdelwahab, N. S., Abdelrahman, M. M., & Derayea, S. M. (2020). Spectrofluorimetric approach for determination of citicoline in the presence of co-formulated piracetam through fluorescence quenching of eosin Y. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 236, 118337. https://doi.org/10.1016/j.saa.2020.118337
Omar MA, et al. Spectrofluorimetric Approach for Determination of Citicoline in the Presence of Co-formulated Piracetam Through Fluorescence Quenching of Eosin Y. Spectrochim Acta A Mol Biomol Spectrosc. 2020 Aug 5;236:118337. PubMed PMID: 32320917.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spectrofluorimetric approach for determination of citicoline in the presence of co-formulated piracetam through fluorescence quenching of eosin Y. AU - Omar,Mahmoud A, AU - Ahmed,Amal B, AU - Abdelwahab,Nada S, AU - Abdelrahman,Maha M, AU - Derayea,Sayed M, Y1 - 2020/04/02/ PY - 2020/03/19/received PY - 2020/03/31/revised PY - 2020/03/31/accepted PY - 2020/4/23/pubmed PY - 2020/4/23/medline PY - 2020/4/23/entrez KW - Citicoline KW - Eosin Y KW - Pharmaceutical formulations KW - Piracetam KW - Spectrofluorimetric SP - 118337 EP - 118337 JF - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JO - Spectrochim Acta A Mol Biomol Spectrosc VL - 236 N2 - A simple, sensitive, and precise spectrofluorimetric method has been developed and validated for quantitation of citicoline in its pharmaceutical formulations. The proposed method based on quantitative quenching effect of citicoline on the native fluorescence of Eosin Y via developing of a binary complex reaction between the cited drug and Eosin Y in acidic medium using acetate buffer pH = 3.6. The quenching of the fluorescence of eosin was measured at 540 nm after excitation at 518 nm. Calibration graph was achieved in the range of 300-3000 ng/mL with 0.9996 as correlation coefficient and 291.0 and 93.86 ng/mL as quantitation and detection limits, respectively. The developed method considered as the first developed spectrofluorimetric one for quantitation of citicoline with high sensitivity and validated according to ICH guidelines. The selectivity of the proposed method was investigated by studying the interference of piracetam as co-formulated drug with CIT in pharmaceutical formulation, therefore the developed method could be used for routine quality control of citicoline in its pharmaceutical formulations either alone or in combination with piracetam. SN - 1873-3557 UR - https://www.unboundmedicine.com/medline/citation/32320917/Spectrofluorimetric_approach_for_determination_of_citicoline_in_the_presence_of_co_formulated_piracetam_through_fluorescence_quenching_of_eosin_Y_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-1425(20)30315-2 DB - PRIME DP - Unbound Medicine ER -
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