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Effects of hydrolyzed fish protein and autolyzed yeast as substitutes of fishmeal in the gilthead sea bream (Sparus aurata) diet, on fish intestinal microbiome.
BMC Vet Res. 2020 04 22; 16(1):118.BV

Abstract

BACKGROUND

This study evaluated the effects of partial substitution of dietary fishmeal (FM) with either fish protein hydrolysate (FPH) or autolysed dried yeast (HiCell®, Biorigin, Brazil) on intestinal microbiota of gilthead sea bream (Sparus aurata). A total number of 720 fish of 122.18 ± 6.22 g were fed for 92 days with three different diets in triplicate (3 tanks/diet). A diet based on FM/vegetable meal was used as control. The other two diets were formulated by replacing FM with 5% of either FPH or HiCell®. To analyze the gut microbiota associated to autochthonous and allochthonous microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME pipeline were used.

RESULTS

A total number of 102 OTUs (operational taxonomic units) at 97% identity were identified in fish gut samples collected at the end of feeding trial. Fourteen OTUs constituted the core gut microbiota, i.e. those OTUs found in at least nine out of fifteen samples per group and shared regardless of the diet. Eight OTUs were assigned to Firmicutes represented by Lactobacillus, Staphylococcus, and Bacillus genera, and six to Proteobacteria phylum. Dietary dried yeast autolysate modulated the intestinal microbiota by promoting the growth of some beneficial bacteria. At order level, fish fed yeast showed an enrichment in Bacillales and Clostridiales as compared to the control group, whereas fish fed FPH showed a significantly lower amount of bacteria belonging to Alteromonadales and Enterobacteriales than the other two feeding groups. Although we did not observe any effect of 5% FM replacement with alternative nitrogen sources at phylum level, at lower taxonomical levels, the composition of gut microbiota, in terms of relative abundance of specific taxa, was significantly influenced by the dietary treatment.

CONCLUSIONS

The metabarcoding analysis revealed a clearly intestinal microbiota modulation in response to dietary autolyzed yeast. The abundance of some beneficial bacteria, i.e. indigestible carbohydrate degrading- and SCFA producing bacteria, was positively affected. Autolysed dried yeast obtained by the fermentation of a strain of Saccharomyces cerevisiae could be a valid alternative protein source to FM as well as a valid functional ingredient for aquafeed production [corrected].

Authors+Show Affiliations

Department of Biotechnology and Life Sciences, University of Insubria, Via J.H. Dunant, 3, 21100, Varese, Italy.Department of Biotechnology and Life Sciences, University of Insubria, Via J.H. Dunant, 3, 21100, Varese, Italy.Biorigin Brazil. Rua XV de Novembro, 865, Lençóis Paulista, São Paulo, 18680-900, Brazil.Department of Biotechnology and Life Sciences, University of Insubria, Via J.H. Dunant, 3, 21100, Varese, Italy.VRM srl Naturalleva, Via Sommacampagna, 63/D, 37137, Verona, Italy.Department of Biotechnology and Life Sciences, University of Insubria, Via J.H. Dunant, 3, 21100, Varese, Italy. genciana.terova@uninsubria.it.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32321508

Citation

Rimoldi, S, et al. "Effects of Hydrolyzed Fish Protein and Autolyzed Yeast as Substitutes of Fishmeal in the Gilthead Sea Bream (Sparus Aurata) Diet, On Fish Intestinal Microbiome." BMC Veterinary Research, vol. 16, no. 1, 2020, p. 118.
Rimoldi S, Gini E, Koch JFA, et al. Effects of hydrolyzed fish protein and autolyzed yeast as substitutes of fishmeal in the gilthead sea bream (Sparus aurata) diet, on fish intestinal microbiome. BMC Vet Res. 2020;16(1):118.
Rimoldi, S., Gini, E., Koch, J. F. A., Iannini, F., Brambilla, F., & Terova, G. (2020). Effects of hydrolyzed fish protein and autolyzed yeast as substitutes of fishmeal in the gilthead sea bream (Sparus aurata) diet, on fish intestinal microbiome. BMC Veterinary Research, 16(1), 118. https://doi.org/10.1186/s12917-020-02335-1
Rimoldi S, et al. Effects of Hydrolyzed Fish Protein and Autolyzed Yeast as Substitutes of Fishmeal in the Gilthead Sea Bream (Sparus Aurata) Diet, On Fish Intestinal Microbiome. BMC Vet Res. 2020 04 22;16(1):118. PubMed PMID: 32321508.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of hydrolyzed fish protein and autolyzed yeast as substitutes of fishmeal in the gilthead sea bream (Sparus aurata) diet, on fish intestinal microbiome. AU - Rimoldi,S, AU - Gini,E, AU - Koch,J F A, AU - Iannini,F, AU - Brambilla,F, AU - Terova,G, Y1 - 2020/04/22/ PY - 2019/12/12/received PY - 2020/04/06/accepted PY - 2020/4/24/entrez PY - 2020/4/24/pubmed PY - 2021/1/6/medline KW - Aquaculture KW - Fish protein hydrolysate, autolyzed yeast KW - Gut microbiota KW - Single cell proteins, fish nutrition SP - 118 EP - 118 JF - BMC veterinary research JO - BMC Vet Res VL - 16 IS - 1 N2 - BACKGROUND: This study evaluated the effects of partial substitution of dietary fishmeal (FM) with either fish protein hydrolysate (FPH) or autolysed dried yeast (HiCell®, Biorigin, Brazil) on intestinal microbiota of gilthead sea bream (Sparus aurata). A total number of 720 fish of 122.18 ± 6.22 g were fed for 92 days with three different diets in triplicate (3 tanks/diet). A diet based on FM/vegetable meal was used as control. The other two diets were formulated by replacing FM with 5% of either FPH or HiCell®. To analyze the gut microbiota associated to autochthonous and allochthonous microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME pipeline were used. RESULTS: A total number of 102 OTUs (operational taxonomic units) at 97% identity were identified in fish gut samples collected at the end of feeding trial. Fourteen OTUs constituted the core gut microbiota, i.e. those OTUs found in at least nine out of fifteen samples per group and shared regardless of the diet. Eight OTUs were assigned to Firmicutes represented by Lactobacillus, Staphylococcus, and Bacillus genera, and six to Proteobacteria phylum. Dietary dried yeast autolysate modulated the intestinal microbiota by promoting the growth of some beneficial bacteria. At order level, fish fed yeast showed an enrichment in Bacillales and Clostridiales as compared to the control group, whereas fish fed FPH showed a significantly lower amount of bacteria belonging to Alteromonadales and Enterobacteriales than the other two feeding groups. Although we did not observe any effect of 5% FM replacement with alternative nitrogen sources at phylum level, at lower taxonomical levels, the composition of gut microbiota, in terms of relative abundance of specific taxa, was significantly influenced by the dietary treatment. CONCLUSIONS: The metabarcoding analysis revealed a clearly intestinal microbiota modulation in response to dietary autolyzed yeast. The abundance of some beneficial bacteria, i.e. indigestible carbohydrate degrading- and SCFA producing bacteria, was positively affected. Autolysed dried yeast obtained by the fermentation of a strain of Saccharomyces cerevisiae could be a valid alternative protein source to FM as well as a valid functional ingredient for aquafeed production [corrected]. SN - 1746-6148 UR - https://www.unboundmedicine.com/medline/citation/32321508/Effects_of_hydrolyzed_fish_protein_and_autolyzed_yeast_as_substitutes_of_fishmeal_in_the_gilthead_sea_bream__Sparus_aurata__diet_on_fish_intestinal_microbiome_ L2 - https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02335-1 DB - PRIME DP - Unbound Medicine ER -