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Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts.
Toxins (Basel). 2020 04 21; 12(4)T

Abstract

Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal).

Authors+Show Affiliations

Biomedical Research Center (CINBIO), Department of Analytical and Food Chemistry, University of Vigo, Campus Universitario de Vigo, 36310 Vigo, Spain.Ifremer, DYNECO, Laboratoire Phycotoxines, Rue de l'Île d'Yeu, 44311 Nantes, France.Biomedical Research Center (CINBIO), Department of Analytical and Food Chemistry, University of Vigo, Campus Universitario de Vigo, 36310 Vigo, Spain.IPMA-Portuguese Institute of the Sea and Atmosphere, Av. Brasília, 1449-006 Lisbon, Portugal.Biomedical Research Center (CINBIO), Department of Analytical and Food Chemistry, University of Vigo, Campus Universitario de Vigo, 36310 Vigo, Spain.Ifremer, DYNECO, Laboratoire Phycotoxines, Rue de l'Île d'Yeu, 44311 Nantes, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32326183

Citation

Estevez, Pablo, et al. "Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused By Fish From European Atlantic Coasts." Toxins, vol. 12, no. 4, 2020.
Estevez P, Sibat M, Leão-Martins JM, et al. Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts. Toxins (Basel). 2020;12(4).
Estevez, P., Sibat, M., Leão-Martins, J. M., Reis Costa, P., Gago-Martínez, A., & Hess, P. (2020). Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts. Toxins, 12(4). https://doi.org/10.3390/toxins12040267
Estevez P, et al. Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused By Fish From European Atlantic Coasts. Toxins (Basel). 2020 04 21;12(4) PubMed PMID: 32326183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts. AU - Estevez,Pablo, AU - Sibat,Manoella, AU - Leão-Martins,José Manuel, AU - Reis Costa,Pedro, AU - Gago-Martínez,Ana, AU - Hess,Philipp, Y1 - 2020/04/21/ PY - 2020/03/31/received PY - 2020/04/16/revised PY - 2020/04/18/accepted PY - 2020/4/25/entrez PY - 2020/4/25/pubmed PY - 2021/2/26/medline KW - C-CTX1 KW - HRMS KW - Q-TOF KW - ciguatera poisoning KW - ciguatoxins KW - fragmentation pathways JF - Toxins JO - Toxins (Basel) VL - 12 IS - 4 N2 - Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal). SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/32326183/Liquid_Chromatography_Coupled_to_High_Resolution_Mass_Spectrometry_for_the_Confirmation_of_Caribbean_Ciguatoxin_1_as_the_Main_Toxin_Responsible_for_Ciguatera_Poisoning_Caused_by_Fish_from_European_Atlantic_Coasts_ L2 - https://www.mdpi.com/resolver?pii=toxins12040267 DB - PRIME DP - Unbound Medicine ER -