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Sodium Nitroprusside Enhances Absorption in the Rat Jejunum via the Transcellular Route.
J Membr Biol. 2020 06; 253(3):221-228.JM

Abstract

It was reported that nitric oxide (NO) donors increased the permeability of water-soluble compounds across intestinal membrane with neither loss of cell viability nor release of lactate dehydrogenase. Therefore, the detail mechanism of action of NO donors on the gastrointestinal membrane has yet to be clarified. We previously reported the possibility of the enhancing effect of the NO donor on the membrane permeability via transcellular route. The purpose of this study is to clarify the mechanism of the membrane permeation-enhancing effect via the transcellular route by sodium nitroprusside (SNP), which is one of the NO donors. The effect of SNP on membrane permeation was examined by the in vitro sac method using rat jejunum. SNP increased the membrane permeation of rhodamine 123 same as using N-acetyl-L-cysteine and dithiothreitol which removes unstirred water layer (UWL). Moreover, SNP increased the membrane permeation of antipyrine and β-naphthol, which are transcellular markers. And it was also investigated the expression levels of mucins (MUCs) which are construction component of UWL and the slight change of MUCs expression by SNP was shown. It was suggested that the expression balance of MUCs is necessary to regulate transcellular permeation, and SNP may affect to UWL. This finding was considered useful for highly lipophilic drugs for which membrane permeation is restricted by the UWL.

Authors+Show Affiliations

Division of Clinical Pharmaceutics, Department of Pharmaceutical Sciences, Nihon Pharmaceutical University, 10281, Komuro, Ina-machi, Kitaadachi-gun, Saitama, 362-0806, Japan. y-takizawa@nichiyaku.ac.jp. Department of Drug Absorption and Pharmacokinetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan. y-takizawa@nichiyaku.ac.jp.Department of Drug Absorption and Pharmacokinetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.Department of Drug Absorption and Pharmacokinetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.Department of Drug Absorption and Pharmacokinetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.Laboratory of Molecular Pharmaceutics and Technology, Faculty of Pharmacy, Takasaki University of Health and Welfare, 60 Nakaorui-machi, Takasaki, Gunma, 370-0033, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32328687

Citation

Takizawa, Yusuke, et al. "Sodium Nitroprusside Enhances Absorption in the Rat Jejunum Via the Transcellular Route." The Journal of Membrane Biology, vol. 253, no. 3, 2020, pp. 221-228.
Takizawa Y, Tobe Y, Sakamoto N, et al. Sodium Nitroprusside Enhances Absorption in the Rat Jejunum via the Transcellular Route. J Membr Biol. 2020;253(3):221-228.
Takizawa, Y., Tobe, Y., Sakamoto, N., Sakamoto, J., & Hayashi, M. (2020). Sodium Nitroprusside Enhances Absorption in the Rat Jejunum via the Transcellular Route. The Journal of Membrane Biology, 253(3), 221-228. https://doi.org/10.1007/s00232-020-00118-1
Takizawa Y, et al. Sodium Nitroprusside Enhances Absorption in the Rat Jejunum Via the Transcellular Route. J Membr Biol. 2020;253(3):221-228. PubMed PMID: 32328687.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sodium Nitroprusside Enhances Absorption in the Rat Jejunum via the Transcellular Route. AU - Takizawa,Yusuke, AU - Tobe,Yoshifusa, AU - Sakamoto,Nasa, AU - Sakamoto,Junya, AU - Hayashi,Masahiro, Y1 - 2020/04/23/ PY - 2019/12/13/received PY - 2020/04/15/accepted PY - 2020/4/25/pubmed PY - 2021/8/14/medline PY - 2020/4/25/entrez KW - Mucin KW - Nitric oxide KW - Sodium nitroprusside KW - Transcellular route KW - Unstirred water layer SP - 221 EP - 228 JF - The Journal of membrane biology JO - J Membr Biol VL - 253 IS - 3 N2 - It was reported that nitric oxide (NO) donors increased the permeability of water-soluble compounds across intestinal membrane with neither loss of cell viability nor release of lactate dehydrogenase. Therefore, the detail mechanism of action of NO donors on the gastrointestinal membrane has yet to be clarified. We previously reported the possibility of the enhancing effect of the NO donor on the membrane permeability via transcellular route. The purpose of this study is to clarify the mechanism of the membrane permeation-enhancing effect via the transcellular route by sodium nitroprusside (SNP), which is one of the NO donors. The effect of SNP on membrane permeation was examined by the in vitro sac method using rat jejunum. SNP increased the membrane permeation of rhodamine 123 same as using N-acetyl-L-cysteine and dithiothreitol which removes unstirred water layer (UWL). Moreover, SNP increased the membrane permeation of antipyrine and β-naphthol, which are transcellular markers. And it was also investigated the expression levels of mucins (MUCs) which are construction component of UWL and the slight change of MUCs expression by SNP was shown. It was suggested that the expression balance of MUCs is necessary to regulate transcellular permeation, and SNP may affect to UWL. This finding was considered useful for highly lipophilic drugs for which membrane permeation is restricted by the UWL. SN - 1432-1424 UR - https://www.unboundmedicine.com/medline/citation/32328687/Sodium_Nitroprusside_Enhances_Absorption_in_the_Rat_Jejunum_via_the_Transcellular_Route_ L2 - https://dx.doi.org/10.1007/s00232-020-00118-1 DB - PRIME DP - Unbound Medicine ER -