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The Comparative Analysis of Antiviral Activity of Native and Modified Fucoidans from Brown Algae Fucus evanescens In Vitro and In Vivo.
Mar Drugs. 2020 Apr 22; 18(4)MD

Abstract

The enzymatic depolymerization of fucoidans from brown algae allowed the production of their standardized derivatives with different biological activities. This work aimed to compare the antiviral activities of native (FeF) and modified with enzyme (FeHMP) fucoidans from F. evanescens. The cytotoxicity and antiviral activities of the FeF and FeHMP against herpes viruses (HSV-1, HSV-2), enterovirus (ECHO-1), and human immunodeficiency virus (HIV-1) in Vero and human MT-4 cell lines were examined by methylthiazolyltetrazolium bromide (MTT) and cytopathic effect (CPE) reduction assays, respectively. The efficacy of fucoidans in vivo was evaluated in the outbred mice model of vaginitis caused by HSV-2. We have shown that both FeF and FeHMP significantly inhibited virus-induced CPE in vitro and were more effective against HSV. FeF exhibited antiviral activity against HSV-2 with a selective index (SI) > 40, and FeHMP with SI ˃ 20, when they were added before virus infection or at the early stages of the HSV-2 lifecycle. Furthermore, in vivo studies showed that after intraperitoneal administration (10 mg/kg), both FeF and FeHMP protected mice from lethal intravaginal HSV-2 infection to approximately the same degree (44-56%). Thus, FeF and FeHMP have comparable potency against several DNA and RNA viruses, allowing us to consider the studied fucoidans as promising broad-spectrum antivirals.

Authors+Show Affiliations

G.P. Somov Institute of Epidemiology and Microbiology, 690087 Vladivostok, Russia.G.B. Elyakov Pacific Institute of Bioorganic Chemistry, 690022 Vladivostok, Russia.I.I. Mechnikov Research Institute of Vaccines and Sera, 105064 Moscow, Russia.I.I. Mechnikov Research Institute of Vaccines and Sera, 105064 Moscow, Russia.G.P. Somov Institute of Epidemiology and Microbiology, 690087 Vladivostok, Russia.G.P. Somov Institute of Epidemiology and Microbiology, 690087 Vladivostok, Russia.I.I. Mechnikov Research Institute of Vaccines and Sera, 105064 Moscow, Russia.I.I. Mechnikov Research Institute of Vaccines and Sera, 105064 Moscow, Russia.I.I. Mechnikov Research Institute of Vaccines and Sera, 105064 Moscow, Russia.G.B. Elyakov Pacific Institute of Bioorganic Chemistry, 690022 Vladivostok, Russia.G.P. Somov Institute of Epidemiology and Microbiology, 690087 Vladivostok, Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32331442

Citation

Krylova, Natalya V., et al. "The Comparative Analysis of Antiviral Activity of Native and Modified Fucoidans From Brown Algae Fucus Evanescens in Vitro and in Vivo." Marine Drugs, vol. 18, no. 4, 2020.
Krylova NV, Ermakova SP, Lavrov VF, et al. The Comparative Analysis of Antiviral Activity of Native and Modified Fucoidans from Brown Algae Fucus evanescens In Vitro and In Vivo. Mar Drugs. 2020;18(4).
Krylova, N. V., Ermakova, S. P., Lavrov, V. F., Leneva, I. A., Kompanets, G. G., Iunikhina, O. V., Nosik, M. N., Ebralidze, L. K., Falynskova, I. N., Silchenko, A. S., & Zaporozhets, T. S. (2020). The Comparative Analysis of Antiviral Activity of Native and Modified Fucoidans from Brown Algae Fucus evanescens In Vitro and In Vivo. Marine Drugs, 18(4). https://doi.org/10.3390/md18040224
Krylova NV, et al. The Comparative Analysis of Antiviral Activity of Native and Modified Fucoidans From Brown Algae Fucus Evanescens in Vitro and in Vivo. Mar Drugs. 2020 Apr 22;18(4) PubMed PMID: 32331442.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Comparative Analysis of Antiviral Activity of Native and Modified Fucoidans from Brown Algae Fucus evanescens In Vitro and In Vivo. AU - Krylova,Natalya V, AU - Ermakova,Svetlana P, AU - Lavrov,Vyacheslav F, AU - Leneva,Irina A, AU - Kompanets,Galina G, AU - Iunikhina,Olga V, AU - Nosik,Marina N, AU - Ebralidze,Linna K, AU - Falynskova,Irina N, AU - Silchenko,Artem S, AU - Zaporozhets,Tatyana S, Y1 - 2020/04/22/ PY - 2020/03/19/received PY - 2020/04/13/revised PY - 2020/04/20/accepted PY - 2020/4/26/entrez PY - 2020/4/26/pubmed PY - 2020/4/26/medline KW - ECHO-1 KW - HIV KW - HSV-1 KW - HSV-2 KW - antiviral activity KW - fucoidans JF - Marine drugs JO - Mar Drugs VL - 18 IS - 4 N2 - The enzymatic depolymerization of fucoidans from brown algae allowed the production of their standardized derivatives with different biological activities. This work aimed to compare the antiviral activities of native (FeF) and modified with enzyme (FeHMP) fucoidans from F. evanescens. The cytotoxicity and antiviral activities of the FeF and FeHMP against herpes viruses (HSV-1, HSV-2), enterovirus (ECHO-1), and human immunodeficiency virus (HIV-1) in Vero and human MT-4 cell lines were examined by methylthiazolyltetrazolium bromide (MTT) and cytopathic effect (CPE) reduction assays, respectively. The efficacy of fucoidans in vivo was evaluated in the outbred mice model of vaginitis caused by HSV-2. We have shown that both FeF and FeHMP significantly inhibited virus-induced CPE in vitro and were more effective against HSV. FeF exhibited antiviral activity against HSV-2 with a selective index (SI) > 40, and FeHMP with SI ˃ 20, when they were added before virus infection or at the early stages of the HSV-2 lifecycle. Furthermore, in vivo studies showed that after intraperitoneal administration (10 mg/kg), both FeF and FeHMP protected mice from lethal intravaginal HSV-2 infection to approximately the same degree (44-56%). Thus, FeF and FeHMP have comparable potency against several DNA and RNA viruses, allowing us to consider the studied fucoidans as promising broad-spectrum antivirals. SN - 1660-3397 UR - https://www.unboundmedicine.com/medline/citation/32331442/The_Comparative_Analysis_of_Antiviral_Activity_of_Native_and_Modified_Fucoidans_from_Brown_Algae_Fucus_evanescens_In_Vitro_and_In_Vivo L2 - https://www.mdpi.com/resolver?pii=md18040224 DB - PRIME DP - Unbound Medicine ER -
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