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[Electroacupuncture improves cutaneous allergic reaction by inhibiting degranulation of intrape-ritoneal mast cells, MAPK signaling and inflammatory factor levels in urticaria rats].
Zhen Ci Yan Jiu. 2020 Apr 25; 45(4):299-304.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on degranulation of intraperitoneal mast cells (MCs) and expression of mitogen-activated protein kinase (MAPK) signaling related proteins, tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6) in urticaria rats, so as to reveal its mechanisms underlying improvement of urticaria.

METHODS

Thirty-two SD rats were randomly divided into control,model,EA and medication groups (n=8 in each group). The urticaria model was established by using passive cutaneous anaphylaxis (PCA) reaction method. EA (2 Hz /15 Hz, 1 mA) was applied to bilateral "Zusanli"(ST36), "Quchi "(LI11) and "Xuehai"(SP10) for 20 min,once daily for 7 consecutive days before antigen attack. Rats of the medication group were treated by gavage of Loratadine(1 mg•kg-1•d-1)for 7 days. The diameter of cutaneous Evan's blue spots was measured to evaluate the severity of PCA. Intraperitoneal fluid smears were prepared to observe the degranulation state of MCs. The contents of TNF-α and IL-6 in the intraperitoneal fluid were detected by ELISA, and the expression of extracellular signal-regulated kinase (ERK), phosphorylated (p)-ERK, c-Jun N-terminal kinase (JNK), p-JNK, P38MAPK and p-P38MAPK of the acquired intraperitoneal MCs was detected by Western blot.

RESULTS

The diameter of cutaneous Evan's blue spot was significantly increased in the model group than that in the control group (P<0.01), and considerably decreased in both EA and medication groups compared with the model group(P<0.01). After modeling,the percentage of degranulated MCs, contents of TNF-α and IL-6, and expression levels of ERK, p-ERK, JNK, p-JNK, P38MAPK and p-P38MAPK were remarkably increased in the mo-del group than those in the control group (P<0.01, P<0.05). After the treatment, the percentage of degranulated MCs, contents of TNF-α and IL-6, and expression levels of p-ERK, JNK, p-JNK and p-P38MAPK were obviously decreased in both EA and medication groups relevant to the model group (P<0.01, P<0.05), while no significant changes were found in the expression of ERK in both EA and medication groups, and P38MAPK in the EA group. Compared with the model and EA groups, expression levels of P38MAPK were down-regulated in the medication group (P<0.05).

CONCLUSION

EA can reduce skin allergic reaction in rats with urticaria, which may be related to its effects in inhibiting the degranulation of intraperitoneal MCs, down-regulating the expression of MAPK signaling-related proteins and the level of pro-inflammatory factors TNF-α and IL-6 in intraperitoneal MCs.

Authors+Show Affiliations

Graduate School, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China.State Key Laboratory of Natural and Biomimetic Drugs of Peking University, Beijing 100191.Second Affiliated Hospital, Liaoning University of Traditional Chinese Medicine, Shenyang 110032.Basic Medical Sciences, Liaoning University of Traditional Chinese Medicine, Shenyang 110032.Graduate School, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China.Graduate School, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China.Graduate School, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China.School of Acupuncture-moxibustion and Tuina, Liaoning University of Traditional Chinese Medicine, Shenyang 110032.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

32333535

Citation

Zhang, Xiao-Hong, et al. "[Electroacupuncture Improves Cutaneous Allergic Reaction By Inhibiting Degranulation of Intrape-ritoneal Mast Cells, MAPK Signaling and Inflammatory Factor Levels in Urticaria Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 45, no. 4, 2020, pp. 299-304.
Zhang XH, Li FF, Qi Y, et al. [Electroacupuncture improves cutaneous allergic reaction by inhibiting degranulation of intrape-ritoneal mast cells, MAPK signaling and inflammatory factor levels in urticaria rats]. Zhen Ci Yan Jiu. 2020;45(4):299-304.
Zhang, X. H., Li, F. F., Qi, Y., Ming, C. R., Li, Y., Pan, S. T., Liu, S. J., & Ma, T. M. (2020). [Electroacupuncture improves cutaneous allergic reaction by inhibiting degranulation of intrape-ritoneal mast cells, MAPK signaling and inflammatory factor levels in urticaria rats]. Zhen Ci Yan Jiu = Acupuncture Research, 45(4), 299-304. https://doi.org/10.13702/j.1000-0607.190263
Zhang XH, et al. [Electroacupuncture Improves Cutaneous Allergic Reaction By Inhibiting Degranulation of Intrape-ritoneal Mast Cells, MAPK Signaling and Inflammatory Factor Levels in Urticaria Rats]. Zhen Ci Yan Jiu. 2020 Apr 25;45(4):299-304. PubMed PMID: 32333535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Electroacupuncture improves cutaneous allergic reaction by inhibiting degranulation of intrape-ritoneal mast cells, MAPK signaling and inflammatory factor levels in urticaria rats]. AU - Zhang,Xiao-Hong, AU - Li,Fei-Fei, AU - Qi,Yue, AU - Ming,Cai-Rong, AU - Li,Ying, AU - Pan,Si-Teng, AU - Liu,Si-Jia, AU - Ma,Tie-Ming, PY - 2020/4/26/entrez PY - 2020/4/26/pubmed PY - 2020/6/26/medline KW - Electroacupuncture KW - MAPK signaling pathway KW - Mast cell degranulation KW - Urticaria SP - 299 EP - 304 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 45 IS - 4 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on degranulation of intraperitoneal mast cells (MCs) and expression of mitogen-activated protein kinase (MAPK) signaling related proteins, tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6) in urticaria rats, so as to reveal its mechanisms underlying improvement of urticaria. METHODS: Thirty-two SD rats were randomly divided into control,model,EA and medication groups (n=8 in each group). The urticaria model was established by using passive cutaneous anaphylaxis (PCA) reaction method. EA (2 Hz /15 Hz, 1 mA) was applied to bilateral "Zusanli"(ST36), "Quchi "(LI11) and "Xuehai"(SP10) for 20 min,once daily for 7 consecutive days before antigen attack. Rats of the medication group were treated by gavage of Loratadine(1 mg•kg-1•d-1)for 7 days. The diameter of cutaneous Evan's blue spots was measured to evaluate the severity of PCA. Intraperitoneal fluid smears were prepared to observe the degranulation state of MCs. The contents of TNF-α and IL-6 in the intraperitoneal fluid were detected by ELISA, and the expression of extracellular signal-regulated kinase (ERK), phosphorylated (p)-ERK, c-Jun N-terminal kinase (JNK), p-JNK, P38MAPK and p-P38MAPK of the acquired intraperitoneal MCs was detected by Western blot. RESULTS: The diameter of cutaneous Evan's blue spot was significantly increased in the model group than that in the control group (P<0.01), and considerably decreased in both EA and medication groups compared with the model group(P<0.01). After modeling,the percentage of degranulated MCs, contents of TNF-α and IL-6, and expression levels of ERK, p-ERK, JNK, p-JNK, P38MAPK and p-P38MAPK were remarkably increased in the mo-del group than those in the control group (P<0.01, P<0.05). After the treatment, the percentage of degranulated MCs, contents of TNF-α and IL-6, and expression levels of p-ERK, JNK, p-JNK and p-P38MAPK were obviously decreased in both EA and medication groups relevant to the model group (P<0.01, P<0.05), while no significant changes were found in the expression of ERK in both EA and medication groups, and P38MAPK in the EA group. Compared with the model and EA groups, expression levels of P38MAPK were down-regulated in the medication group (P<0.05). CONCLUSION: EA can reduce skin allergic reaction in rats with urticaria, which may be related to its effects in inhibiting the degranulation of intraperitoneal MCs, down-regulating the expression of MAPK signaling-related proteins and the level of pro-inflammatory factors TNF-α and IL-6 in intraperitoneal MCs. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/32333535/[Electroacupuncture_improves_cutaneous_allergic_reaction_by_inhibiting_degranulation_of_intrape_ritoneal_mast_cells_MAPK_signaling_and_inflammatory_factor_levels_in_urticaria_rats]_ L2 - https://medlineplus.gov/hives.html DB - PRIME DP - Unbound Medicine ER -