Tags

Type your tag names separated by a space and hit enter

Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract.
Lab Chip. 2020 05 05; 20(9):1621-1627.LC

Abstract

Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.

Authors+Show Affiliations

Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA. bcunning@illinois.edu.Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA.RAIN Incubator, Tacoma, Washington, USA.Department of Interdisciplinary Arts and Sciences & The Center for Urban Waters, University of Washington Tacoma, Washington, USA.Department of Interdisciplinary Arts and Sciences & The Center for Urban Waters, University of Washington Tacoma, Washington, USA.RAIN Incubator, Tacoma, Washington, USA and Department of Interdisciplinary Arts and Sciences & The Center for Urban Waters, University of Washington Tacoma, Washington, USA.Department of Animal Sciences, University of Illinois at Urbana-Champaign, Illinois, USA.Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA. bcunning@illinois.edu and Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA.Private equine veterinarian, Kentucky, USA.Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA. bcunning@illinois.edu and Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

32334422

Citation

Sun, Fu, et al. "Smartphone-based Multiplex 30-minute Nucleic Acid Test of Live Virus From Nasal Swab Extract." Lab On a Chip, vol. 20, no. 9, 2020, pp. 1621-1627.
Sun F, Ganguli A, Nguyen J, et al. Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract. Lab Chip. 2020;20(9):1621-1627.
Sun, F., Ganguli, A., Nguyen, J., Brisbin, R., Shanmugam, K., Hirschberg, D. L., Wheeler, M. B., Bashir, R., Nash, D. M., & Cunningham, B. T. (2020). Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract. Lab On a Chip, 20(9), 1621-1627. https://doi.org/10.1039/d0lc00304b
Sun F, et al. Smartphone-based Multiplex 30-minute Nucleic Acid Test of Live Virus From Nasal Swab Extract. Lab Chip. 2020 05 5;20(9):1621-1627. PubMed PMID: 32334422.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract. AU - Sun,Fu, AU - Ganguli,Anurup, AU - Nguyen,Judy, AU - Brisbin,Ryan, AU - Shanmugam,Krithika, AU - Hirschberg,David L, AU - Wheeler,Matthew B, AU - Bashir,Rashid, AU - Nash,David M, AU - Cunningham,Brian T, PY - 2020/4/26/pubmed PY - 2020/4/26/medline PY - 2020/4/26/entrez SP - 1621 EP - 1627 JF - Lab on a chip JO - Lab Chip VL - 20 IS - 9 N2 - Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes. SN - 1473-0189 UR - https://www.unboundmedicine.com/medline/citation/32334422/Smartphone-based_multiplex_30-minute_nucleic_acid_test_of_live_virus_from_nasal_swab_extract L2 - https://doi.org/10.1039/d0lc00304b DB - PRIME DP - Unbound Medicine ER -