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Burkholderia pseudomallei was Identified in a Melioidosis Aneurysm using Polymerase Chain Reaction Targeting 23S rRNA.
Ann Vasc Surg. 2020 Oct; 68:569.e13-569.e20.AV

Abstract

Melioidosis abdominal aortic aneurysm and splenic abscesses lead to poor prognosis and high mortality rate as high as 50% due to delayed/missed diagnosis. We describe an attempt to identify Burkholderia pseudomallei immediately, which was confirmed by polymerase chain reaction (PCR) and gene sequence analysis of 23S rRNA gene. PCR is not only an unambiguous identification of B. pseudomallei but also a rapid detection because B. pseudomallei may not be readily isolated. For patients of melioidosis abdominal aortic aneurysm with spleen abscess, prolonged antibiotic therapy, splenectomy and artificial vessel replacement provided an excellent result in our study. The progression, roentgenographic findings and histopathology character of melioidosis are similar to those of tuberculosis disease. PCR is useful to differentiate B. pseudomallei from Mycobacterium tuberculosis.

Authors+Show Affiliations

The Infective Disease Department and Tropical Medicine Research Unit, Affiliated Hainan Hospital of Hainan Medical University, Haikou, Hainan Province, People's Republic of China. Electronic address: shilideng@126.com.Acupuncture and Massage Institute, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, People's Republic of China.The Infective Disease Department and Tropical Medicine Research Unit, Affiliated Hainan Hospital of Hainan Medical University, Haikou, Hainan Province, People's Republic of China.

Pub Type(s)

Case Reports

Language

eng

PubMed ID

32339680

Citation

Shi, Li, et al. "Burkholderia Pseudomallei Was Identified in a Melioidosis Aneurysm Using Polymerase Chain Reaction Targeting 23S RRNA." Annals of Vascular Surgery, vol. 68, 2020, pp. 569.e13-569.e20.
Shi L, Chen J, Yi G. Burkholderia pseudomallei was Identified in a Melioidosis Aneurysm using Polymerase Chain Reaction Targeting 23S rRNA. Ann Vasc Surg. 2020;68:569.e13-569.e20.
Shi, L., Chen, J., & Yi, G. (2020). Burkholderia pseudomallei was Identified in a Melioidosis Aneurysm using Polymerase Chain Reaction Targeting 23S rRNA. Annals of Vascular Surgery, 68, e13-e20. https://doi.org/10.1016/j.avsg.2020.04.035
Shi L, Chen J, Yi G. Burkholderia Pseudomallei Was Identified in a Melioidosis Aneurysm Using Polymerase Chain Reaction Targeting 23S RRNA. Ann Vasc Surg. 2020;68:569.e13-569.e20. PubMed PMID: 32339680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Burkholderia pseudomallei was Identified in a Melioidosis Aneurysm using Polymerase Chain Reaction Targeting 23S rRNA. AU - Shi,Li, AU - Chen,Jie, AU - Yi,Gao, Y1 - 2020/04/25/ PY - 2019/08/02/received PY - 2020/03/28/revised PY - 2020/04/04/accepted PY - 2020/4/28/pubmed PY - 2020/11/25/medline PY - 2020/4/28/entrez SP - 569.e13 EP - 569.e20 JF - Annals of vascular surgery JO - Ann Vasc Surg VL - 68 N2 - Melioidosis abdominal aortic aneurysm and splenic abscesses lead to poor prognosis and high mortality rate as high as 50% due to delayed/missed diagnosis. We describe an attempt to identify Burkholderia pseudomallei immediately, which was confirmed by polymerase chain reaction (PCR) and gene sequence analysis of 23S rRNA gene. PCR is not only an unambiguous identification of B. pseudomallei but also a rapid detection because B. pseudomallei may not be readily isolated. For patients of melioidosis abdominal aortic aneurysm with spleen abscess, prolonged antibiotic therapy, splenectomy and artificial vessel replacement provided an excellent result in our study. The progression, roentgenographic findings and histopathology character of melioidosis are similar to those of tuberculosis disease. PCR is useful to differentiate B. pseudomallei from Mycobacterium tuberculosis. SN - 1615-5947 UR - https://www.unboundmedicine.com/medline/citation/32339680/Burkholderia_pseudomallei_was_Identified_in_a_Melioidosis_Aneurysm_using_Polymerase_Chain_Reaction_Targeting_23S_rRNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0890-5096(20)30354-X DB - PRIME DP - Unbound Medicine ER -