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In vitro α-amylase and α-glucosidase Inhibition, Antioxidant, Anti- Inflammatory Activity and GC-MS Profiling of Avicennia alba Blume.
Comb Chem High Throughput Screen. 2020; 23(9):945-954.CC

Abstract

BACKGROUND

Avicennia alba Blume, is a well-known mangrove plant used in traditional medicinal practices for several human ailments.

OBJECTIVE

The study aimed at the evaluation of antidiabetic, antioxidant, anti-inflammatory and cytotoxic activities of A. alba ethanolic leaf (AAL) and bark (AAB) extract along with phytochemical investigation.

METHODS

In vitro antidiabetic study was done by α-amylase, α-glucosidase enzyme inhibition assay; antioxidant study by DPPH, ABTS, superoxide, and metal chelating assays, antiinflammatory study by protein denaturation assay. The cytotoxicity study was done on TC1 murine cell line. Further, GC-MS analysis was carried out for AAL extracts.

RESULTS

AAL exhibited better antidiabetic activities with IC50 values of 1.18 and 0.87 mg/ml against α-amylase and α-glucosidase enzymes respectively. The AAL exhibited better ABTS, superoxide scavenging and metal chelating potential with IC50 values of 0.095, 0.127 and 0.444 mg/ml. However, AAB showed higher DPPH scavenging potential with IC50 value of 0.163 mg/ml. The AAL also exhibited higher protein denaturation potential with IC50 value of 0.370 mg/ml. The bark extract exhibited better cytotoxic activity as compared to leaf extracts on the TC1 murine cell line. The phytochemical study revealed higher total phenol (25.64 mg GAE/g), flavonoid (205.09 mg QE/g), and tannin content (251.17 mg GAE/g) in AAL. The GC-MS analysis revealed the presence of several compounds in AAL extract.

CONCLUSION

The result of the present study highlights the antidiabetic, antioxidant and cytotoxic activities of mangrove plant Avicennia alba.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Das SK
Department of Biotechnology, College of Engineering and Technology, Techno Campus, Ghatikia, Bhubaneswar 751003, Odisha, India.
Dash S
Department of Biotechnology, College of Engineering and Technology, Techno Campus, Ghatikia, Bhubaneswar 751003, Odisha, India.
Thatoi H
Department of Biotechnology, North Odisha University, Sriramchandra Vihar, Takatpur, Baripada 757003, Odisha, India.
Patra JK
Research Institute of Biotechnology & Medical Converged Science, Dongguk University-Seoul, Goyangsi 10326, Korea.

MeSH

AnimalsAnti-Inflammatory AgentsAntioxidantsAvicenniaCell LineCell SurvivalDrug DiscoveryGas Chromatography-Mass SpectrometryGlycoside Hydrolase InhibitorsHumansHypoglycemic AgentsMicePhenolsPhytochemicalsPlant Components, AerialPlant ExtractsTanninsalpha-Amylasesalpha-Glucosidases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32342807

Citation

Das, Swagat K., et al. "In Vitro Α-amylase and Α-glucosidase Inhibition, Antioxidant, Anti- Inflammatory Activity and GC-MS Profiling of Avicennia Alba Blume." Combinatorial Chemistry & High Throughput Screening, vol. 23, no. 9, 2020, pp. 945-954.
Das SK, Dash S, Thatoi H, et al. In vitro α-amylase and α-glucosidase Inhibition, Antioxidant, Anti- Inflammatory Activity and GC-MS Profiling of Avicennia alba Blume. Comb Chem High Throughput Screen. 2020;23(9):945-954.
Das, S. K., Dash, S., Thatoi, H., & Patra, J. K. (2020). In vitro α-amylase and α-glucosidase Inhibition, Antioxidant, Anti- Inflammatory Activity and GC-MS Profiling of Avicennia alba Blume. Combinatorial Chemistry & High Throughput Screening, 23(9), 945-954. https://doi.org/10.2174/1386207323666200428081748
Das SK, et al. In Vitro Α-amylase and Α-glucosidase Inhibition, Antioxidant, Anti- Inflammatory Activity and GC-MS Profiling of Avicennia Alba Blume. Comb Chem High Throughput Screen. 2020;23(9):945-954. PubMed PMID: 32342807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro α-amylase and α-glucosidase Inhibition, Antioxidant, Anti- Inflammatory Activity and GC-MS Profiling of Avicennia alba Blume. AU - Das,Swagat K, AU - Dash,Sagarika, AU - Thatoi,Hrudayanath, AU - Patra,Jayanta K, PY - 2019/11/26/received PY - 2020/01/14/revised PY - 2020/01/28/accepted PY - 2020/4/29/pubmed PY - 2021/7/3/medline PY - 2020/4/29/entrez KW - Avicennia alba KW - GC-MS analysis KW - anti-inflammatory KW - antidiabetic KW - antioxidant KW - cytotoxic SP - 945 EP - 954 JF - Combinatorial chemistry & high throughput screening JO - Comb Chem High Throughput Screen VL - 23 IS - 9 N2 - BACKGROUND: Avicennia alba Blume, is a well-known mangrove plant used in traditional medicinal practices for several human ailments. OBJECTIVE: The study aimed at the evaluation of antidiabetic, antioxidant, anti-inflammatory and cytotoxic activities of A. alba ethanolic leaf (AAL) and bark (AAB) extract along with phytochemical investigation. METHODS: In vitro antidiabetic study was done by α-amylase, α-glucosidase enzyme inhibition assay; antioxidant study by DPPH, ABTS, superoxide, and metal chelating assays, antiinflammatory study by protein denaturation assay. The cytotoxicity study was done on TC1 murine cell line. Further, GC-MS analysis was carried out for AAL extracts. RESULTS: AAL exhibited better antidiabetic activities with IC50 values of 1.18 and 0.87 mg/ml against α-amylase and α-glucosidase enzymes respectively. The AAL exhibited better ABTS, superoxide scavenging and metal chelating potential with IC50 values of 0.095, 0.127 and 0.444 mg/ml. However, AAB showed higher DPPH scavenging potential with IC50 value of 0.163 mg/ml. The AAL also exhibited higher protein denaturation potential with IC50 value of 0.370 mg/ml. The bark extract exhibited better cytotoxic activity as compared to leaf extracts on the TC1 murine cell line. The phytochemical study revealed higher total phenol (25.64 mg GAE/g), flavonoid (205.09 mg QE/g), and tannin content (251.17 mg GAE/g) in AAL. The GC-MS analysis revealed the presence of several compounds in AAL extract. CONCLUSION: The result of the present study highlights the antidiabetic, antioxidant and cytotoxic activities of mangrove plant Avicennia alba. SN - 1875-5402 UR - https://www.unboundmedicine.com/medline/citation/32342807/In_vitro_α_amylase_and_α_glucosidase_Inhibition_Antioxidant_Anti__Inflammatory_Activity_and_GC_MS_Profiling_of_Avicennia_alba_Blume_ DB - PRIME DP - Unbound Medicine ER -

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