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Isolation and purification of acidic polysaccharides from Agaricus blazei Murill and evaluation of their lipid-lowering mechanism.
Int J Biol Macromol. 2020 Aug 15; 157:276-287.IJ

Abstract

Polysaccharides are important active constituents of Agaricus blazei Morrill. In the present study, WABM-A was isolated from WABM using DEAE-cellulose, and subsequently purified using sepharose CL-6B to obtain the acidic polysaccharide WABM-A-b. WABM-A-b is mainly composed of Glc dextran, with a molecular weight of 10 KDa and β-1,6-D-Glcp as its main chain. The results of in vivo experiments show that in comparison with the MG, WABM-A significantly reduced the serum levels of TC, TG, and LDL-C, increased the serum levels of HDL-C (P < 0.01), and upregulated the liver expression of PPARγ, LXRα, ABCA1, and ABCG1 in rats with hyperlipidemia (P < 0.05). The results of in vitro experiments show that in comparison with the MG group, WABM-A-b-H significantly reduced the levels of TC and TG in HepG2 cells induced by oleic acid (P < 0.01), and significantly upregulated the protein expression of PPARγ, LXRα, ABCA1, and ABCG1 (P < 0.05). The present study demonstrates that WABM-A-b is an acidic glucan with lipid-lowering activity. The lipid-lowering mechanism of WABM-A-b is via the activation of the PPARγ/LXRα/ABCA1/ABCG1 cholesterol metabolism pathway. This is the first time that the hypolipidemic effect of Agaricus blazei Morrill acidic polysaccharides has been reported.

Authors+Show Affiliations

College of Pharmacy, Beihua University, Jilin 132013, China.College of Pharmacy, Beihua University, Jilin 132013, China.General Hospital of the People's Liberation Army, Beijing 100853, China.College of Pharmacy, Beihua University, Jilin 132013, China.College of Pharmacy, Beihua University, Jilin 132013, China.College of Pharmacy, Beihua University, Jilin 132013, China.College of Pharmacy, Beihua University, Jilin 132013, China. Electronic address: alp960@126.com.College of Pharmacy, Beihua University, Jilin 132013, China. Electronic address: dupeige2001@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32344083

Citation

Li, Yuxin, et al. "Isolation and Purification of Acidic Polysaccharides From Agaricus Blazei Murill and Evaluation of Their Lipid-lowering Mechanism." International Journal of Biological Macromolecules, vol. 157, 2020, pp. 276-287.
Li Y, Sheng Y, Lu X, et al. Isolation and purification of acidic polysaccharides from Agaricus blazei Murill and evaluation of their lipid-lowering mechanism. Int J Biol Macromol. 2020;157:276-287.
Li, Y., Sheng, Y., Lu, X., Guo, X., Xu, G., Han, X., An, L., & Du, P. (2020). Isolation and purification of acidic polysaccharides from Agaricus blazei Murill and evaluation of their lipid-lowering mechanism. International Journal of Biological Macromolecules, 157, 276-287. https://doi.org/10.1016/j.ijbiomac.2020.04.190
Li Y, et al. Isolation and Purification of Acidic Polysaccharides From Agaricus Blazei Murill and Evaluation of Their Lipid-lowering Mechanism. Int J Biol Macromol. 2020 Aug 15;157:276-287. PubMed PMID: 32344083.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and purification of acidic polysaccharides from Agaricus blazei Murill and evaluation of their lipid-lowering mechanism. AU - Li,Yuxin, AU - Sheng,Yu, AU - Lu,Xuechun, AU - Guo,Xiao, AU - Xu,Guangyu, AU - Han,Xiao, AU - An,Liping, AU - Du,Peige, Y1 - 2020/04/25/ PY - 2019/12/09/received PY - 2020/04/21/revised PY - 2020/04/22/accepted PY - 2020/4/29/pubmed PY - 2021/3/13/medline PY - 2020/4/29/entrez KW - Agaricus blazei Murrill KW - Lipid-lowering KW - Polysaccharide SP - 276 EP - 287 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 157 N2 - Polysaccharides are important active constituents of Agaricus blazei Morrill. In the present study, WABM-A was isolated from WABM using DEAE-cellulose, and subsequently purified using sepharose CL-6B to obtain the acidic polysaccharide WABM-A-b. WABM-A-b is mainly composed of Glc dextran, with a molecular weight of 10 KDa and β-1,6-D-Glcp as its main chain. The results of in vivo experiments show that in comparison with the MG, WABM-A significantly reduced the serum levels of TC, TG, and LDL-C, increased the serum levels of HDL-C (P < 0.01), and upregulated the liver expression of PPARγ, LXRα, ABCA1, and ABCG1 in rats with hyperlipidemia (P < 0.05). The results of in vitro experiments show that in comparison with the MG group, WABM-A-b-H significantly reduced the levels of TC and TG in HepG2 cells induced by oleic acid (P < 0.01), and significantly upregulated the protein expression of PPARγ, LXRα, ABCA1, and ABCG1 (P < 0.05). The present study demonstrates that WABM-A-b is an acidic glucan with lipid-lowering activity. The lipid-lowering mechanism of WABM-A-b is via the activation of the PPARγ/LXRα/ABCA1/ABCG1 cholesterol metabolism pathway. This is the first time that the hypolipidemic effect of Agaricus blazei Morrill acidic polysaccharides has been reported. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/32344083/Isolation_and_purification_of_acidic_polysaccharides_from_Agaricus_blazei_Murill_and_evaluation_of_their_lipid_lowering_mechanism_ DB - PRIME DP - Unbound Medicine ER -