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In vitro intestinal toxicity of commercially available spray disinfectant products advertised to contain colloidal silver.
Sci Total Environ. 2020 Aug 01; 728:138611.ST

Abstract

The use of colloidal silver-containing products as dietary supplements, immune boosters and surface disinfectants has increased in recent years which has elevated the potential for human exposure to silver nanoparticles and ions. Product mislabeling and long-term use of these products may put consumers at risk for adverse health outcomes including argyria. This study assessed several physical and chemical characteristics of five commercial products as well as their cytotoxicity using a rat intestinal epithelial cell (IEC-6) model. Concentrations of silver were determined for both the soluble and particulate fractions of the products. Primary particle size distribution and elemental composition were determined by transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS), respectively. Hydrodynamic diameters were measured using nanoparticle tracking analysis (NTA) and dynamic light scattering (DLS). The effect of gastrointestinal (GI) simulation on the colloidal silver products was determined using two systems. First, physical and chemical changes of the silver nanoparticles in these products was assessed after exposure to Synthetic Stomach Fluid (SSF) resulting in particle agglomeration, and the appearance of AgCl on the surfaces and between particles. IEC-6 cells were exposed for 24 h to dilutions of the products and assessed for cell viability. The products were also treated with a three-stage simulated GI system (stomach and intestinal fluids) prior to exposure of the IEC-6 cells to the isolated silver nanoparticles. Cell viability was affected by each of the consumer products. Based on the silver nitrate and commercial silver nanoparticle dose response, the cytotoxicity for each of the colloidal silver products was attributed to the particulate silver, soluble silver or non‑silver matrix constituents.

Authors+Show Affiliations

Watershed and Ecosystem Characterization Division, Center for Environmental Measurement and Modeling, Office of Research and Development, USEPA, RTP, NC 27711, United States. Electronic address: rogers.kim@epa.gov.Oak Ridge Institute for Science and Education, Research Triangle Park, NC 27711, United States; Chemical Characterization and Exposure Division, Center for Computational Toxicology and Exposure, Office of Research and Development, USEPA, RTP, NC 27711, United States.Watershed and Ecosystem Characterization Division, Center for Environmental Measurement and Modeling, Office of Research and Development, USEPA, RTP, NC 27711, United States.Watershed and Ecosystem Characterization Division, Center for Environmental Measurement and Modeling, Office of Research and Development, USEPA, RTP, NC 27711, United States.Chemical Characterization and Exposure Division, Center for Computational Toxicology and Exposure, Office of Research and Development, USEPA, RTP, NC 27711, United States.Watershed and Ecosystem Characterization Division, Center for Environmental Measurement and Modeling, Office of Research and Development, USEPA, RTP, NC 27711, United States.National Institute for Occupational Safety and Health, Morgantown, WV 26506, United States.National Institute for Occupational Safety and Health, Morgantown, WV 26506, United States.National Institute for Occupational Safety and Health, Morgantown, WV 26506, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32344222

Citation

Rogers, Kim R., et al. "In Vitro Intestinal Toxicity of Commercially Available Spray Disinfectant Products Advertised to Contain Colloidal Silver." The Science of the Total Environment, vol. 728, 2020, p. 138611.
Rogers KR, Henson TE, Navratilova J, et al. In vitro intestinal toxicity of commercially available spray disinfectant products advertised to contain colloidal silver. Sci Total Environ. 2020;728:138611.
Rogers, K. R., Henson, T. E., Navratilova, J., Surette, M., Hughes, M. F., Bradham, K. D., Stefaniak, A. B., Knepp, A. K., & Bowers, L. (2020). In vitro intestinal toxicity of commercially available spray disinfectant products advertised to contain colloidal silver. The Science of the Total Environment, 728, 138611. https://doi.org/10.1016/j.scitotenv.2020.138611
Rogers KR, et al. In Vitro Intestinal Toxicity of Commercially Available Spray Disinfectant Products Advertised to Contain Colloidal Silver. Sci Total Environ. 2020 Aug 1;728:138611. PubMed PMID: 32344222.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro intestinal toxicity of commercially available spray disinfectant products advertised to contain colloidal silver. AU - Rogers,Kim R, AU - Henson,Taylor E, AU - Navratilova,Jana, AU - Surette,Mark, AU - Hughes,Michael F, AU - Bradham,Karen D, AU - Stefaniak,Aleksandr B, AU - Knepp,Alycia K, AU - Bowers,Lauren, Y1 - 2020/04/14/ PY - 2020/02/19/received PY - 2020/04/08/revised PY - 2020/04/08/accepted PY - 2020/4/29/pubmed PY - 2020/7/11/medline PY - 2020/4/29/entrez KW - Bioavailability KW - Colloidal silver KW - Silver nanoparticles SP - 138611 EP - 138611 JF - The Science of the total environment JO - Sci Total Environ VL - 728 N2 - The use of colloidal silver-containing products as dietary supplements, immune boosters and surface disinfectants has increased in recent years which has elevated the potential for human exposure to silver nanoparticles and ions. Product mislabeling and long-term use of these products may put consumers at risk for adverse health outcomes including argyria. This study assessed several physical and chemical characteristics of five commercial products as well as their cytotoxicity using a rat intestinal epithelial cell (IEC-6) model. Concentrations of silver were determined for both the soluble and particulate fractions of the products. Primary particle size distribution and elemental composition were determined by transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS), respectively. Hydrodynamic diameters were measured using nanoparticle tracking analysis (NTA) and dynamic light scattering (DLS). The effect of gastrointestinal (GI) simulation on the colloidal silver products was determined using two systems. First, physical and chemical changes of the silver nanoparticles in these products was assessed after exposure to Synthetic Stomach Fluid (SSF) resulting in particle agglomeration, and the appearance of AgCl on the surfaces and between particles. IEC-6 cells were exposed for 24 h to dilutions of the products and assessed for cell viability. The products were also treated with a three-stage simulated GI system (stomach and intestinal fluids) prior to exposure of the IEC-6 cells to the isolated silver nanoparticles. Cell viability was affected by each of the consumer products. Based on the silver nitrate and commercial silver nanoparticle dose response, the cytotoxicity for each of the colloidal silver products was attributed to the particulate silver, soluble silver or non‑silver matrix constituents. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/32344222/In_vitro_intestinal_toxicity_of_commercially_available_spray_disinfectant_products_advertised_to_contain_colloidal_silver L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(20)32127-6 DB - PRIME DP - Unbound Medicine ER -