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Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies.
Molecules. 2020 Apr 24; 25(8)M

Abstract

Seaweeds are a rich source of protein and can contain up to 47% on the dry weight basis. It is challenging to extract proteins from the raw biomass of seaweed due to resilient cell-wall complexes. Four species of macroalgae were used in this study-two brown, Fucus vesiculosus and Alaria esculenta, and two red, Palmaria palmata and Chondrus crispus. Three treatments were applied individually to the macroalgal species: (I) high-pressure processing (HPP); (II) laboratory autoclave processing and (III) a classical sonication and salting out method. The protein, ash and lipid contents of the resulting extracts were estimated. Yields of protein recovered ranged from 3.2% for Fucus vesiculosus pre-treated with high pressure processing to 28.9% protein recovered for Chondrus crispus treated with the classical method. The yields of protein recovered using the classical, HPP and autoclave pre-treatments applied to Fucus vesiculosus were 35.1, 23.7% and 24.3%, respectively; yields from Alaria esculenta were 18.2%, 15.0% and 17.1% respectively; yields from Palmaria palmata were 12.5%, 14.9% and 21.5% respectively, and finally, yields from Chondrus crispus were 35.2%, 16.1% and 21.9%, respectively. These results demonstrate that while macroalgal proteins may be extracted using either physical or enzymatic methods, the specific extraction procedure should be tailored to individual species.

Authors+Show Affiliations

The Food BioSciences Department, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland. School of Biological and Health Sciences, Technological University Dublin (TU Dublin)-City Campus, Kevin Street, Dublin 2, Ireland.School of Biological and Health Sciences, Technological University Dublin (TU Dublin)-City Campus, Kevin Street, Dublin 2, Ireland.School of Biological and Health Sciences, Technological University Dublin (TU Dublin)-City Campus, Kevin Street, Dublin 2, Ireland.The Food BioSciences Department, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32344706

Citation

O' Connor, Jack, et al. "Extraction of Protein From Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies." Molecules (Basel, Switzerland), vol. 25, no. 8, 2020.
O' Connor J, Meaney S, Williams GA, et al. Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies. Molecules. 2020;25(8).
O' Connor, J., Meaney, S., Williams, G. A., & Hayes, M. (2020). Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies. Molecules (Basel, Switzerland), 25(8). https://doi.org/10.3390/molecules25082005
O' Connor J, et al. Extraction of Protein From Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies. Molecules. 2020 Apr 24;25(8) PubMed PMID: 32344706.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies. AU - O' Connor,Jack, AU - Meaney,Steve, AU - Williams,Gwilym A, AU - Hayes,Maria, Y1 - 2020/04/24/ PY - 2020/03/19/received PY - 2020/04/18/revised PY - 2020/04/20/accepted PY - 2020/4/30/entrez PY - 2020/4/30/pubmed PY - 2021/1/29/medline KW - autoclave KW - high pressure processing KW - proteins KW - seaweeds KW - solubility KW - total and free amino acids KW - traditional protein extraction JF - Molecules (Basel, Switzerland) JO - Molecules VL - 25 IS - 8 N2 - Seaweeds are a rich source of protein and can contain up to 47% on the dry weight basis. It is challenging to extract proteins from the raw biomass of seaweed due to resilient cell-wall complexes. Four species of macroalgae were used in this study-two brown, Fucus vesiculosus and Alaria esculenta, and two red, Palmaria palmata and Chondrus crispus. Three treatments were applied individually to the macroalgal species: (I) high-pressure processing (HPP); (II) laboratory autoclave processing and (III) a classical sonication and salting out method. The protein, ash and lipid contents of the resulting extracts were estimated. Yields of protein recovered ranged from 3.2% for Fucus vesiculosus pre-treated with high pressure processing to 28.9% protein recovered for Chondrus crispus treated with the classical method. The yields of protein recovered using the classical, HPP and autoclave pre-treatments applied to Fucus vesiculosus were 35.1, 23.7% and 24.3%, respectively; yields from Alaria esculenta were 18.2%, 15.0% and 17.1% respectively; yields from Palmaria palmata were 12.5%, 14.9% and 21.5% respectively, and finally, yields from Chondrus crispus were 35.2%, 16.1% and 21.9%, respectively. These results demonstrate that while macroalgal proteins may be extracted using either physical or enzymatic methods, the specific extraction procedure should be tailored to individual species. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/32344706/Extraction_of_Protein_from_Four_Different_Seaweeds_Using_Three_Different_Physical_Pre_Treatment_Strategies_ L2 - https://www.mdpi.com/resolver?pii=molecules25082005 DB - PRIME DP - Unbound Medicine ER -