Tags

Type your tag names separated by a space and hit enter

A Strategy for Personalized Treatment of iPS-Retinal Immune Rejections Assessed in Cynomolgus Monkey Models.
Int J Mol Sci. 2020 Apr 27; 21(9)IJ

Abstract

Recently, we successfully transplanted an autograft, or major histocompatibility complex (MHC)-matched allografts, from induced-pluripotent-stem-cell-derived retinal pigment epithelial (iPSC-RPE) cells in patients with age-related macular degeneration. However, there was an issue regarding immune rejection after transplantation. In this study, we established a preoperational in vitro "drug-lymphocytes-grafts immune reaction (Drug-LGIR)" test to determine the medication for immune rejection using host immunocompetent cells (lymphocytes) and transplant cells (target iPSC-RPE cells) together with different medications. The adequacy of the test was assessed by in vivo transplantation in monkey models together with medication based on in vitro data. In the results of Drug-LGIR tests, some drugs exhibited significant suppression of RPE cell-related allogeneic reactions, while other drugs did not, and the efficacy of each drug differed among the recipient monkeys. Based on the results of Drug-LGIR, we applied cyclosporine A or local steroid (triamcinolone) therapy to two monkeys, and successfully suppressed RPE-related immune rejections with RPE grafts, which survived without any signs of rejection under drug administration. We propose that our new preoperational in vitro Drug-LGIR test, which specifies the most efficacious medication for each recipient, is useful for controlling immune attacks with personalized treatment for each patient after retinal transplantation.

Authors+Show Affiliations

Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.Department of Ophthalmology, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama 701-0114, Japan.Department of Ophthalmology & Visual Science, Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, 1-5-45, Yushima, Bunkyo-Ku, Tokyo 113-8510, Japan.Department of Ophthalmology, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.Department of Ophthalmology, Kobe City Eye Hospital, 2-1-8 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.Department of Ophthalmology, Kobe City Eye Hospital, 2-1-8 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32349277

Citation

Fujii, Shota, et al. "A Strategy for Personalized Treatment of iPS-Retinal Immune Rejections Assessed in Cynomolgus Monkey Models." International Journal of Molecular Sciences, vol. 21, no. 9, 2020.
Fujii S, Sugita S, Futatsugi Y, et al. A Strategy for Personalized Treatment of iPS-Retinal Immune Rejections Assessed in Cynomolgus Monkey Models. Int J Mol Sci. 2020;21(9).
Fujii, S., Sugita, S., Futatsugi, Y., Ishida, M., Edo, A., Makabe, K., Kamao, H., Iwasaki, Y., Sakaguchi, H., Hirami, Y., Kurimoto, Y., & Takahashi, M. (2020). A Strategy for Personalized Treatment of iPS-Retinal Immune Rejections Assessed in Cynomolgus Monkey Models. International Journal of Molecular Sciences, 21(9). https://doi.org/10.3390/ijms21093077
Fujii S, et al. A Strategy for Personalized Treatment of iPS-Retinal Immune Rejections Assessed in Cynomolgus Monkey Models. Int J Mol Sci. 2020 Apr 27;21(9) PubMed PMID: 32349277.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Strategy for Personalized Treatment of iPS-Retinal Immune Rejections Assessed in Cynomolgus Monkey Models. AU - Fujii,Shota, AU - Sugita,Sunao, AU - Futatsugi,Yoko, AU - Ishida,Masaaki, AU - Edo,Ayaka, AU - Makabe,Kenichi, AU - Kamao,Hiroyuki, AU - Iwasaki,Yuko, AU - Sakaguchi,Hirokazu, AU - Hirami,Yasuhiko, AU - Kurimoto,Yasuo, AU - Takahashi,Masayo, Y1 - 2020/04/27/ PY - 2020/04/13/received PY - 2020/04/25/revised PY - 2020/04/26/accepted PY - 2020/5/1/entrez PY - 2020/5/1/pubmed PY - 2021/2/2/medline KW - drug KW - iPS cells KW - immune rejection KW - retinal pigment epithelial cells KW - transplantation JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 9 N2 - Recently, we successfully transplanted an autograft, or major histocompatibility complex (MHC)-matched allografts, from induced-pluripotent-stem-cell-derived retinal pigment epithelial (iPSC-RPE) cells in patients with age-related macular degeneration. However, there was an issue regarding immune rejection after transplantation. In this study, we established a preoperational in vitro "drug-lymphocytes-grafts immune reaction (Drug-LGIR)" test to determine the medication for immune rejection using host immunocompetent cells (lymphocytes) and transplant cells (target iPSC-RPE cells) together with different medications. The adequacy of the test was assessed by in vivo transplantation in monkey models together with medication based on in vitro data. In the results of Drug-LGIR tests, some drugs exhibited significant suppression of RPE cell-related allogeneic reactions, while other drugs did not, and the efficacy of each drug differed among the recipient monkeys. Based on the results of Drug-LGIR, we applied cyclosporine A or local steroid (triamcinolone) therapy to two monkeys, and successfully suppressed RPE-related immune rejections with RPE grafts, which survived without any signs of rejection under drug administration. We propose that our new preoperational in vitro Drug-LGIR test, which specifies the most efficacious medication for each recipient, is useful for controlling immune attacks with personalized treatment for each patient after retinal transplantation. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/32349277/A_Strategy_for_Personalized_Treatment_of_iPS_Retinal_Immune_Rejections_Assessed_in_Cynomolgus_Monkey_Models_ L2 - https://www.mdpi.com/resolver?pii=ijms21093077 DB - PRIME DP - Unbound Medicine ER -