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The influence of balanced complex chromosomal rearrangements on preimplantation embryonic development potential and molecular karyotype.
BMC Genomics. 2020 Apr 29; 21(1):326.BG

Abstract

BACKGROUND

Balanced complex chromosome rearrangements (BCCR) are balanced chromosomal structural aberrations that involve two or more chromosomes and at least three breakpoints. It is very rare in the population. The objective is to explore the difference of influence of three types of BCCR on early embryonic development and molecular karyotype.

RESULTS

Twelve couples were recruited including four couples of three-way rearrangements carriers (group A), three couples of double two-way translocations carriers (group B) and five couples of exceptional CCR carriers (group C). A total of 243 oocytes were retrievedin the seventeen preimplantation genetic testing (PGT) cycles, and 207 of these were available for fertilization. After intracytoplasmic sperm injection, 181oocytes normally fertilized. The rates of embryos forming on day3 in three groups were 87.88, 97.78 and77.14%, which was significantly different (P = 0.01). Compared with group B, the rate of embryo formation was statistically significantly lower in group C (P = 0.01). Furthermore, the rates of high-quality blastocysts in three group were 14.71, 48.15 and 62.96%, respectively, which was significantly different (P = 0.00). Compared with group B andC, the rate of high-quality blastocysts in group A was statistically significantly lower (P = 0.00;P = 0.00). Comprehensive chromosome analysis was performed on 83 embryos, including 75 trophectodermcellsand 8 blastomeres. Except 7 embryos failed to amplify, 9.01%embryos were diagnosed as euploidy, and 90.91% were diagnosed as abnormal. As for group A, the euploid embryo rate was 10.71%and the abnormal embryo rate was 89.29%. In group B,the euploid embryo rate was 3.85%, the abnormal embryo rate was 96.15%. The euploid embryo rate was 13.04%, the abnormal embryo rate was 86.96% in group C. There were no significant differences among the three groups (P = 0.55).

CONCLUSIONS

The lowest rate of high quality blastocysts has been for three-way rearrangements and the lowest rate of euploidy has been for double two-way translocations, although no significant difference. Different types of BCCR maybe have little effect on the embryonic molecular karyotype. The difference of influence of BCCR on early embryonic developmentandmolecular karyotypeshould be further studied.

Authors+Show Affiliations

Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, China. lgvigor@126.com.Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, China.Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, China.Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, China.Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, China.Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, China.Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, China. syp2008@vip.sina.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32349655

Citation

Li, Gang, et al. "The Influence of Balanced Complex Chromosomal Rearrangements On Preimplantation Embryonic Development Potential and Molecular Karyotype." BMC Genomics, vol. 21, no. 1, 2020, p. 326.
Li G, Shi W, Niu W, et al. The influence of balanced complex chromosomal rearrangements on preimplantation embryonic development potential and molecular karyotype. BMC Genomics. 2020;21(1):326.
Li, G., Shi, W., Niu, W., Xu, J., Guo, Y., Su, Y., & Sun, Y. (2020). The influence of balanced complex chromosomal rearrangements on preimplantation embryonic development potential and molecular karyotype. BMC Genomics, 21(1), 326. https://doi.org/10.1186/s12864-020-6731-9
Li G, et al. The Influence of Balanced Complex Chromosomal Rearrangements On Preimplantation Embryonic Development Potential and Molecular Karyotype. BMC Genomics. 2020 Apr 29;21(1):326. PubMed PMID: 32349655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The influence of balanced complex chromosomal rearrangements on preimplantation embryonic development potential and molecular karyotype. AU - Li,Gang, AU - Shi,Weiyi, AU - Niu,Wenbin, AU - Xu,Jiawei, AU - Guo,Yihong, AU - Su,Yingchun, AU - Sun,Yingpu, Y1 - 2020/04/29/ PY - 2019/09/09/received PY - 2020/04/14/accepted PY - 2020/5/1/entrez PY - 2020/5/1/pubmed PY - 2020/5/1/medline KW - Assisted reproductive technology KW - Balanced complex chromosome rearrangements KW - Next-generation sequencing technology KW - Preimplantation genetic testing SP - 326 EP - 326 JF - BMC genomics JO - BMC Genomics VL - 21 IS - 1 N2 - BACKGROUND: Balanced complex chromosome rearrangements (BCCR) are balanced chromosomal structural aberrations that involve two or more chromosomes and at least three breakpoints. It is very rare in the population. The objective is to explore the difference of influence of three types of BCCR on early embryonic development and molecular karyotype. RESULTS: Twelve couples were recruited including four couples of three-way rearrangements carriers (group A), three couples of double two-way translocations carriers (group B) and five couples of exceptional CCR carriers (group C). A total of 243 oocytes were retrievedin the seventeen preimplantation genetic testing (PGT) cycles, and 207 of these were available for fertilization. After intracytoplasmic sperm injection, 181oocytes normally fertilized. The rates of embryos forming on day3 in three groups were 87.88, 97.78 and77.14%, which was significantly different (P = 0.01). Compared with group B, the rate of embryo formation was statistically significantly lower in group C (P = 0.01). Furthermore, the rates of high-quality blastocysts in three group were 14.71, 48.15 and 62.96%, respectively, which was significantly different (P = 0.00). Compared with group B andC, the rate of high-quality blastocysts in group A was statistically significantly lower (P = 0.00;P = 0.00). Comprehensive chromosome analysis was performed on 83 embryos, including 75 trophectodermcellsand 8 blastomeres. Except 7 embryos failed to amplify, 9.01%embryos were diagnosed as euploidy, and 90.91% were diagnosed as abnormal. As for group A, the euploid embryo rate was 10.71%and the abnormal embryo rate was 89.29%. In group B,the euploid embryo rate was 3.85%, the abnormal embryo rate was 96.15%. The euploid embryo rate was 13.04%, the abnormal embryo rate was 86.96% in group C. There were no significant differences among the three groups (P = 0.55). CONCLUSIONS: The lowest rate of high quality blastocysts has been for three-way rearrangements and the lowest rate of euploidy has been for double two-way translocations, although no significant difference. Different types of BCCR maybe have little effect on the embryonic molecular karyotype. The difference of influence of BCCR on early embryonic developmentandmolecular karyotypeshould be further studied. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/32349655/The_influence_of_balanced_complex_chromosomal_rearrangements_on_preimplantation_embryonic_development_potential_and_molecular_karyotype L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6731-9 DB - PRIME DP - Unbound Medicine ER -
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