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Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches.
BMC Genomics. 2020 Apr 29; 21(1):331.BG

Abstract

BACKGROUND

Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today.

RESULTS

We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, Hirudo orientalis, and Hirudo verbana. Differential expression analysis verified by proteomics identified salivary cell-specific gene expression, many of which encode previously unknown salivary components. However, the genes encoding known anticoagulants have been found to be expressed not only in salivary cells. The function-related analysis of the unique salivary cell genes enabled an update of the concept of interactions between salivary proteins and components of haemostasis.

CONCLUSIONS

Here we report a genome draft of Hirudo medicinalis and describe identification of novel salivary proteins and new homologs of genes encoding known anticoagulants in transcriptomes of three medicinal leech species. Our data provide new insights in genetics of blood-feeding lifestyle in leeches.

Authors+Show Affiliations

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia. daniorerio34@gmail.com.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov str, Moscow, 119334, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia. Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia.Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia. Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkina str, Moscow, 119991, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia. Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, 19 Bol'shoi Karetnyi per, Moscow, 127051, Russia. Skolkovo Institute of Science and Technology, 3 Nobelya Ulitsa str, Moscow, 121205, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia. Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str, Moscow, 117997, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str, Moscow, 117997, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.V.N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, 10 Pogodinskaja str, Moscow, 119832, Russia.Faculty of Biology, Lomonosov Moscow State University, 1-12 Leninskie Gory, Moscow, 119991, Russia.I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation (Sechenovskiy University), Trubetskaya str., 8-2, Moscow, 119991, Russia.A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, 19 Bol'shoi Karetnyi per, Moscow, 127051, Russia. Skolkovo Institute of Science and Technology, 3 Nobelya Ulitsa str, Moscow, 121205, Russia. Faculty of Computer Science, National Research University Higher School of Economics, 20 Myasnitskaya str, Moscow, 101000, Russia. Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 1-73 Leninskie Gory, Moscow, 119991, Russia.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia. Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia.I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation (Sechenovskiy University), Trubetskaya str., 8-2, Moscow, 119991, Russia. Functional Pharmacology, Department of Neuroscience, Uppsala University, Husargatan 3, Uppsala, 75124, Sweden.Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia. Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32349672

Citation

Babenko, Vladislav V., et al. "Draft Genome Sequences of Hirudo Medicinalis and Salivary Transcriptome of Three Closely Related Medicinal Leeches." BMC Genomics, vol. 21, no. 1, 2020, p. 331.
Babenko VV, Podgorny OV, Manuvera VA, et al. Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches. BMC Genomics. 2020;21(1):331.
Babenko, V. V., Podgorny, O. V., Manuvera, V. A., Kasianov, A. S., Manolov, A. I., Grafskaia, E. N., Shirokov, D. A., Kurdyumov, A. S., Vinogradov, D. V., Nikitina, A. S., Kovalchuk, S. I., Anikanov, N. A., Butenko, I. O., Pobeguts, O. V., Matyushkina, D. S., Rakitina, D. V., Kostryukova, E. S., Zgoda, V. G., Baskova, I. P., ... Lazarev, V. N. (2020). Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches. BMC Genomics, 21(1), 331. https://doi.org/10.1186/s12864-020-6748-0
Babenko VV, et al. Draft Genome Sequences of Hirudo Medicinalis and Salivary Transcriptome of Three Closely Related Medicinal Leeches. BMC Genomics. 2020 Apr 29;21(1):331. PubMed PMID: 32349672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches. AU - Babenko,Vladislav V, AU - Podgorny,Oleg V, AU - Manuvera,Valentin A, AU - Kasianov,Artem S, AU - Manolov,Alexander I, AU - Grafskaia,Ekaterina N, AU - Shirokov,Dmitriy A, AU - Kurdyumov,Alexey S, AU - Vinogradov,Dmitriy V, AU - Nikitina,Anastasia S, AU - Kovalchuk,Sergey I, AU - Anikanov,Nickolay A, AU - Butenko,Ivan O, AU - Pobeguts,Olga V, AU - Matyushkina,Daria S, AU - Rakitina,Daria V, AU - Kostryukova,Elena S, AU - Zgoda,Victor G, AU - Baskova,Isolda P, AU - Trukhan,Vladimir M, AU - Gelfand,Mikhail S, AU - Govorun,Vadim M, AU - Schiöth,Helgi B, AU - Lazarev,Vassili N, Y1 - 2020/04/29/ PY - 2019/10/03/received PY - 2020/04/21/accepted PY - 2020/5/1/entrez PY - 2020/5/1/pubmed PY - 2020/5/1/medline KW - Anticoagulants KW - Genome KW - Haematophagy KW - Leech H. medicinalis KW - Systems biology SP - 331 EP - 331 JF - BMC genomics JO - BMC Genomics VL - 21 IS - 1 N2 - BACKGROUND: Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. RESULTS: We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, Hirudo orientalis, and Hirudo verbana. Differential expression analysis verified by proteomics identified salivary cell-specific gene expression, many of which encode previously unknown salivary components. However, the genes encoding known anticoagulants have been found to be expressed not only in salivary cells. The function-related analysis of the unique salivary cell genes enabled an update of the concept of interactions between salivary proteins and components of haemostasis. CONCLUSIONS: Here we report a genome draft of Hirudo medicinalis and describe identification of novel salivary proteins and new homologs of genes encoding known anticoagulants in transcriptomes of three medicinal leech species. Our data provide new insights in genetics of blood-feeding lifestyle in leeches. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/32349672/Draft_genome_sequences_of_Hirudo_medicinalis_and_salivary_transcriptome_of_three_closely_related_medicinal_leeches L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6748-0 DB - PRIME DP - Unbound Medicine ER -