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Effects of chronic nicotine exposure on Δ[9]-tetrahydrocannabinol-induced locomotor activity and neural activation in male and female adolescent and adult rats.
Pharmacol Biochem Behav. 2020 07; 194:172931.PB

Abstract

RATIONALE

High rates of comorbid tobacco and cannabis use in adolescents and young adults may be related to functional interactions between the nicotinic cholinergic and cannabinoid systems in the brain during development. This study examined the effects of chronic exposure to nicotine (the psychoactive component in tobacco) on acute exposure to delta-9-tetrahydrocannabinol (THC) (the psychoactive component of cannabis).

METHODS

Male and female adolescent and adult Sprague-Dawley rats (N = 112) were injected daily with nicotine (1 mg/kg, i.p.) or vehicle for 14 days, followed by a 14-day drug-free period. On test day, rats were injected with THC (5 mg/kg, i.p.) or vehicle, locomotor activity was recorded for 2 h, and brains harvested for c-Fos immunoreactivity (IR).

RESULTS

Locomotor activity and c-Fos IR changes induced by THC challenge were altered by nicotine pre-exposure and modified by age and sex. THC-induced suppression of locomotor activity was attenuated by nicotine pre-exposure in adult but not adolescent males. THC-induced suppression of locomotor activity was potentiated by nicotine pre-exposure in female adolescents, with no effects of THC or nicotine observed in female adults. THC increased c-Fos IR in the caudate, nucleus accumbens, stria terminalis, septum, amygdala, hypothalamus, and thalamus. Nicotine pre-exposure potentiated this effect in all regions. Several brain regions showed age and sex differences in c-Fos IR such that expression was greater in adults than adolescents and in females than males.

CONCLUSIONS

Chronic nicotine pre-exposure produces lasting effects on cannabinoid-mediated signalling in the brain and on behaviour that are mediated by age and sex.

FUNDING SUPPORT

NSERC.

Authors+Show Affiliations

Department of Psychology, Wilfrid Laurier University, Waterloo, ON N2L3C5, Canada.Department of Psychology, Wilfrid Laurier University, Waterloo, ON N2L3C5, Canada. Electronic address: lmanwell@wlu.ca.Department of Psychology, Wilfrid Laurier University, Waterloo, ON N2L3C5, Canada.Department of Psychology, Wilfrid Laurier University, Waterloo, ON N2L3C5, Canada.Department of Psychology, Wilfrid Laurier University, Waterloo, ON N2L3C5, Canada.Department of Psychology, Wilfrid Laurier University, Waterloo, ON N2L3C5, Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32353393

Citation

Miladinovic, T, et al. "Effects of Chronic Nicotine Exposure On Δ[9]-tetrahydrocannabinol-induced Locomotor Activity and Neural Activation in Male and Female Adolescent and Adult Rats." Pharmacology, Biochemistry, and Behavior, vol. 194, 2020, p. 172931.
Miladinovic T, Manwell LA, Raaphorst E, et al. Effects of chronic nicotine exposure on Δ[9]-tetrahydrocannabinol-induced locomotor activity and neural activation in male and female adolescent and adult rats. Pharmacol Biochem Behav. 2020;194:172931.
Miladinovic, T., Manwell, L. A., Raaphorst, E., Malecki, S. L., Rana, S. A., & Mallet, P. E. (2020). Effects of chronic nicotine exposure on Δ[9]-tetrahydrocannabinol-induced locomotor activity and neural activation in male and female adolescent and adult rats. Pharmacology, Biochemistry, and Behavior, 194, 172931. https://doi.org/10.1016/j.pbb.2020.172931
Miladinovic T, et al. Effects of Chronic Nicotine Exposure On Δ[9]-tetrahydrocannabinol-induced Locomotor Activity and Neural Activation in Male and Female Adolescent and Adult Rats. Pharmacol Biochem Behav. 2020;194:172931. PubMed PMID: 32353393.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of chronic nicotine exposure on Δ[9]-tetrahydrocannabinol-induced locomotor activity and neural activation in male and female adolescent and adult rats. AU - Miladinovic,T, AU - Manwell,L A, AU - Raaphorst,E, AU - Malecki,S L, AU - Rana,S A, AU - Mallet,P E, Y1 - 2020/04/28/ PY - 2019/06/26/received PY - 2020/03/27/revised PY - 2020/04/11/accepted PY - 2020/5/1/pubmed PY - 2021/3/31/medline PY - 2020/5/1/entrez KW - Adolescent KW - Adult KW - C-Fos gene expression KW - Cross-sensitization KW - Development KW - Drug abuse KW - Locomotor activity KW - Nicotine KW - Rat KW - Tetrahydrocannabinol (THC) SP - 172931 EP - 172931 JF - Pharmacology, biochemistry, and behavior JO - Pharmacol Biochem Behav VL - 194 N2 - RATIONALE: High rates of comorbid tobacco and cannabis use in adolescents and young adults may be related to functional interactions between the nicotinic cholinergic and cannabinoid systems in the brain during development. This study examined the effects of chronic exposure to nicotine (the psychoactive component in tobacco) on acute exposure to delta-9-tetrahydrocannabinol (THC) (the psychoactive component of cannabis). METHODS: Male and female adolescent and adult Sprague-Dawley rats (N = 112) were injected daily with nicotine (1 mg/kg, i.p.) or vehicle for 14 days, followed by a 14-day drug-free period. On test day, rats were injected with THC (5 mg/kg, i.p.) or vehicle, locomotor activity was recorded for 2 h, and brains harvested for c-Fos immunoreactivity (IR). RESULTS: Locomotor activity and c-Fos IR changes induced by THC challenge were altered by nicotine pre-exposure and modified by age and sex. THC-induced suppression of locomotor activity was attenuated by nicotine pre-exposure in adult but not adolescent males. THC-induced suppression of locomotor activity was potentiated by nicotine pre-exposure in female adolescents, with no effects of THC or nicotine observed in female adults. THC increased c-Fos IR in the caudate, nucleus accumbens, stria terminalis, septum, amygdala, hypothalamus, and thalamus. Nicotine pre-exposure potentiated this effect in all regions. Several brain regions showed age and sex differences in c-Fos IR such that expression was greater in adults than adolescents and in females than males. CONCLUSIONS: Chronic nicotine pre-exposure produces lasting effects on cannabinoid-mediated signalling in the brain and on behaviour that are mediated by age and sex. FUNDING SUPPORT: NSERC. SN - 1873-5177 UR - https://www.unboundmedicine.com/medline/citation/32353393/Effects_of_chronic_nicotine_exposure_on_Δ[9]_tetrahydrocannabinol_induced_locomotor_activity_and_neural_activation_in_male_and_female_adolescent_and_adult_rats_ DB - PRIME DP - Unbound Medicine ER -