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Efficient, fast and inexpensive bioassay to monitor benthic microalgae toxicity: Application to Ostreopsis species.
Aquat Toxicol. 2020 Jun; 223:105485.AT

Abstract

Even though HPLC-MS is commonly used to quantify the toxin content of Ostreopsis spp. cells, there is a need to develop easy-to-use toxicological tests to set thresholds during Ostreopsis spp. blooms. The crustacean Artemia has been widely used to evaluate the presence and toxicity of chemicals and biological contaminants and we anticipated that it could also be useful to test Ostreopsis spp. toxicity. Its relevance was first assessed by investigating the variability of the toxic effects among Ostreopsis spp. strains and throughout the dinoflagellate life cycle in combination with chemical analyses of the toxinic content by UHPLC-HRMS. After testing the toxicity of fractions prepared from Ostreopsis spp. cells, the known ova- and paly-toxins were not the only toxic metabolites to Artemia franciscana, indicating that other toxic compounds synthesized by Ostreopsis spp. still remain to be identified. To extend the bioassay to in situ monitoring, the toxicity of the benthic microalgal consortium was tested during a natural bloom of Ostreopsis cf. ovata in the NW Mediterranean Sea. The results highlight the accuracy and sensitivity of the ecotoxicological assay with Artemia franciscana to assess the toxicity of Ostreopsis spp. blooms.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Pavaux AS
Sorbonne Université, CNRS, Laboratoire d'Océanographie de Villefranche, LOV, F-06230, Villefranche-sur-Mer, France. Electronic address: annesophie.pavaux@gmail.com.
Ternon E
Université Côte d'Azur, CNRS, OCA, IRD, Géoazur, 250 rue Albert Einstein, 06560, Valbonne, France.
Dufour L
Sorbonne Université, CNRS, Laboratoire d'Océanographie de Villefranche, LOV, F-06230, Villefranche-sur-Mer, France.
Marro S
Sorbonne Université, CNRS, Laboratoire d'Océanographie de Villefranche, LOV, F-06230, Villefranche-sur-Mer, France.
Gémin MP
IFREMER - Laboratoire Phycotoxines (PHYC), Rue de l'Île d'Yeu, BP 21105, F- 44311, Nantes, France.
Thomas OP
Marine Biodiscovery, School of Chemistry and Ryan Institute, National University of Ireland Galway, University Road, H91 TK33, Galway, Ireland.
Lemée R
Sorbonne Université, CNRS, Laboratoire d'Océanographie de Villefranche, LOV, F-06230, Villefranche-sur-Mer, France.

MeSH

AnimalsArtemiaBiological AssayDinoflagellidaEnvironmental MonitoringMass SpectrometryMediterranean SeaMicroalgaeWater Pollutants, Chemical

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32353662

Citation

Pavaux, Anne-Sophie, et al. "Efficient, Fast and Inexpensive Bioassay to Monitor Benthic Microalgae Toxicity: Application to Ostreopsis Species." Aquatic Toxicology (Amsterdam, Netherlands), vol. 223, 2020, p. 105485.
Pavaux AS, Ternon E, Dufour L, et al. Efficient, fast and inexpensive bioassay to monitor benthic microalgae toxicity: Application to Ostreopsis species. Aquat Toxicol. 2020;223:105485.
Pavaux, A. S., Ternon, E., Dufour, L., Marro, S., Gémin, M. P., Thomas, O. P., & Lemée, R. (2020). Efficient, fast and inexpensive bioassay to monitor benthic microalgae toxicity: Application to Ostreopsis species. Aquatic Toxicology (Amsterdam, Netherlands), 223, 105485. https://doi.org/10.1016/j.aquatox.2020.105485
Pavaux AS, et al. Efficient, Fast and Inexpensive Bioassay to Monitor Benthic Microalgae Toxicity: Application to Ostreopsis Species. Aquat Toxicol. 2020;223:105485. PubMed PMID: 32353662.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficient, fast and inexpensive bioassay to monitor benthic microalgae toxicity: Application to Ostreopsis species. AU - Pavaux,Anne-Sophie, AU - Ternon,Eva, AU - Dufour,Louison, AU - Marro,Sophie, AU - Gémin,Marin-Pierre, AU - Thomas,Olivier P, AU - Lemée,Rodolphe, Y1 - 2020/04/22/ PY - 2019/12/18/received PY - 2020/04/02/revised PY - 2020/04/07/accepted PY - 2020/5/1/pubmed PY - 2020/6/20/medline PY - 2020/5/1/entrez KW - Artemia franciscana KW - Bioassay KW - Ecotoxicity KW - HPLC-HRMS KW - Ostreopsis spp. SP - 105485 EP - 105485 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 223 N2 - Even though HPLC-MS is commonly used to quantify the toxin content of Ostreopsis spp. cells, there is a need to develop easy-to-use toxicological tests to set thresholds during Ostreopsis spp. blooms. The crustacean Artemia has been widely used to evaluate the presence and toxicity of chemicals and biological contaminants and we anticipated that it could also be useful to test Ostreopsis spp. toxicity. Its relevance was first assessed by investigating the variability of the toxic effects among Ostreopsis spp. strains and throughout the dinoflagellate life cycle in combination with chemical analyses of the toxinic content by UHPLC-HRMS. After testing the toxicity of fractions prepared from Ostreopsis spp. cells, the known ova- and paly-toxins were not the only toxic metabolites to Artemia franciscana, indicating that other toxic compounds synthesized by Ostreopsis spp. still remain to be identified. To extend the bioassay to in situ monitoring, the toxicity of the benthic microalgal consortium was tested during a natural bloom of Ostreopsis cf. ovata in the NW Mediterranean Sea. The results highlight the accuracy and sensitivity of the ecotoxicological assay with Artemia franciscana to assess the toxicity of Ostreopsis spp. blooms. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/32353662/Efficient_fast_and_inexpensive_bioassay_to_monitor_benthic_microalgae_toxicity:_Application_to_Ostreopsis_species_ DB - PRIME DP - Unbound Medicine ER -