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Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota-Results from the PAGODA Trial.
Nutrients. 2020 Apr 28; 12(5)N

Abstract

(1)

Background:

Alterations in the structural composition of the human gut microbiota have been identified in various disease entities along with exciting mechanistic clues by reductionist gnotobiotic modeling. Improving health by beneficially modulating an altered microbiota is a promising treatment approach. Prebiotics, substrates selectively used by host microorganisms conferring a health benefit, are broadly used for dietary and clinical interventions. Herein, we sought to investigate the microbiota-modelling effects of the soluble fiber, partially hydrolyzed guar gum (PHGG). (2)

Methods:

We performed a 9 week clinical trial in 20 healthy volunteers that included three weeks of a lead-in period, followed by three weeks of an intervention phase, wherein study subjects received 5 g PHGG up to three times per day, and concluding with a three-week washout period. A stool diary was kept on a daily basis, and clinical data along with serum/plasma and stool samples were collected on a weekly basis. PHGG-induced alterations of the gut microbiota were studied by 16S metagenomics of the V1-V3 and V3-V4 regions. To gain functional insight, we further studied stool metabolites using nuclear magnetic resonance (NMR) spectroscopy. (3)

Results:

In healthy subjects, PHGG had significant effects on stool frequency and consistency. These effects were paralleled by changes in α- (species evenness) and β-diversity (Bray-Curtis distances), along with increasing abundances of metabolites including butyrate, acetate and various amino acids. On a taxonomic level, PHGG intake was associated with a bloom in Ruminococcus, Fusicatenibacter, Faecalibacterium and Bacteroides and a reduction in Roseburia, Lachnospiracea and Blautia. The majority of effects disappeared after stopping the prebiotic and most effects tended to be more pronounced in male participants. (4)

Conclusions:

Herein, we describe novel aspects of the prebiotic PHGG on compositional and functional properties of the healthy human microbiota.

Authors+Show Affiliations

Christian Doppler Laboratory for Mucosal Immunology, Medical University Innsbruck, 6020 Innsbruck, Austria. Department of Internal Medicine I, Gastroenterology, Hepatology, Endocrinology & Metabolism, Medical University Innsbruck, 6020 Innsbruck, Austria.Institute of Pharmacy/Pharmacognosy & Center for Molecular Biosciences Innsbruck, Leopold-Franzens Universität, 6020 Innsbruck, Austria.Christian Doppler Laboratory for Mucosal Immunology, Medical University Innsbruck, 6020 Innsbruck, Austria.Division of Computational Systems Biology, Department of Microbiology, University of Vienna, 1010 Vienna, Austria.General Zoology/Institute of Biology, University Halle, 06108 Halle/Saale, Germany.Division of Computational Systems Biology, Department of Microbiology, University of Vienna, 1010 Vienna, Austria.Christian Doppler Laboratory for Mucosal Immunology, Medical University Innsbruck, 6020 Innsbruck, Austria. Department of Internal Medicine I, Gastroenterology, Hepatology, Endocrinology & Metabolism, Medical University Innsbruck, 6020 Innsbruck, Austria.Institute of Pharmacy/Pharmacognosy & Center for Molecular Biosciences Innsbruck, Leopold-Franzens Universität, 6020 Innsbruck, Austria.Department of Internal Medicine I, Gastroenterology, Hepatology, Endocrinology & Metabolism, Medical University Innsbruck, 6020 Innsbruck, Austria.Institute of Pharmacy/Pharmacognosy & Center for Molecular Biosciences Innsbruck, Leopold-Franzens Universität, 6020 Innsbruck, Austria.Division of Computational Systems Biology, Department of Microbiology, University of Vienna, 1010 Vienna, Austria.Christian Doppler Laboratory for Mucosal Immunology, Medical University Innsbruck, 6020 Innsbruck, Austria. Department of Internal Medicine I, Gastroenterology, Hepatology, Endocrinology & Metabolism, Medical University Innsbruck, 6020 Innsbruck, Austria.

Pub Type(s)

Clinical Trial
Journal Article

Language

eng

PubMed ID

32354152

Citation

Reider, Simon J., et al. "Prebiotic Effects of Partially Hydrolyzed Guar Gum On the Composition and Function of the Human Microbiota-Results From the PAGODA Trial." Nutrients, vol. 12, no. 5, 2020.
Reider SJ, Moosmang S, Tragust J, et al. Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota-Results from the PAGODA Trial. Nutrients. 2020;12(5).
Reider, S. J., Moosmang, S., Tragust, J., Trgovec-Greif, L., Tragust, S., Perschy, L., Przysiecki, N., Sturm, S., Tilg, H., Stuppner, H., Rattei, T., & Moschen, A. R. (2020). Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota-Results from the PAGODA Trial. Nutrients, 12(5). https://doi.org/10.3390/nu12051257
Reider SJ, et al. Prebiotic Effects of Partially Hydrolyzed Guar Gum On the Composition and Function of the Human Microbiota-Results From the PAGODA Trial. Nutrients. 2020 Apr 28;12(5) PubMed PMID: 32354152.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota-Results from the PAGODA Trial. AU - Reider,Simon J, AU - Moosmang,Simon, AU - Tragust,Judith, AU - Trgovec-Greif,Lovro, AU - Tragust,Simon, AU - Perschy,Lorenz, AU - Przysiecki,Nicole, AU - Sturm,Sonja, AU - Tilg,Herbert, AU - Stuppner,Hermann, AU - Rattei,Thomas, AU - Moschen,Alexander R, Y1 - 2020/04/28/ PY - 2020/04/07/received PY - 2020/04/24/revised PY - 2020/04/26/accepted PY - 2020/5/2/entrez PY - 2020/5/2/pubmed PY - 2021/2/16/medline KW - PHGG KW - SCFA KW - fiber KW - microbiota KW - partially hydrolyzed guar gum KW - prebiotics KW - short-chain fatty acids JF - Nutrients JO - Nutrients VL - 12 IS - 5 N2 - (1) Background: Alterations in the structural composition of the human gut microbiota have been identified in various disease entities along with exciting mechanistic clues by reductionist gnotobiotic modeling. Improving health by beneficially modulating an altered microbiota is a promising treatment approach. Prebiotics, substrates selectively used by host microorganisms conferring a health benefit, are broadly used for dietary and clinical interventions. Herein, we sought to investigate the microbiota-modelling effects of the soluble fiber, partially hydrolyzed guar gum (PHGG). (2) Methods: We performed a 9 week clinical trial in 20 healthy volunteers that included three weeks of a lead-in period, followed by three weeks of an intervention phase, wherein study subjects received 5 g PHGG up to three times per day, and concluding with a three-week washout period. A stool diary was kept on a daily basis, and clinical data along with serum/plasma and stool samples were collected on a weekly basis. PHGG-induced alterations of the gut microbiota were studied by 16S metagenomics of the V1-V3 and V3-V4 regions. To gain functional insight, we further studied stool metabolites using nuclear magnetic resonance (NMR) spectroscopy. (3) Results: In healthy subjects, PHGG had significant effects on stool frequency and consistency. These effects were paralleled by changes in α- (species evenness) and β-diversity (Bray-Curtis distances), along with increasing abundances of metabolites including butyrate, acetate and various amino acids. On a taxonomic level, PHGG intake was associated with a bloom in Ruminococcus, Fusicatenibacter, Faecalibacterium and Bacteroides and a reduction in Roseburia, Lachnospiracea and Blautia. The majority of effects disappeared after stopping the prebiotic and most effects tended to be more pronounced in male participants. (4) Conclusions: Herein, we describe novel aspects of the prebiotic PHGG on compositional and functional properties of the healthy human microbiota. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/32354152/Prebiotic_Effects_of_Partially_Hydrolyzed_Guar_Gum_on_the_Composition_and_Function_of_the_Human_Microbiota_Results_from_the_PAGODA_Trial_ L2 - https://www.mdpi.com/resolver?pii=nu12051257 DB - PRIME DP - Unbound Medicine ER -