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Bridging genomics and field research: draft genome sequence of Bacillus thuringiensis CR71, an endophytic bacterium that promotes plant growth and fruit yield in Cucumis sativus L.
3 Biotech. 2020 May; 10(5):220.3B

Abstract

Here we report the draft genome sequence of bacterial strain CR71, consisting of a single chromosome with 5,914,775 base pairs (bp), 34.7% G + C content, and 5733 protein-coding genes. Phylogenetic analysis indicates that the CR71 strain is affiliated with Bacillus thuringiensis species, with an average nucleotide identity > 96% and genome to genome distance > 70%. The genome of B. thuringiensis strain CR71 contains genes potentially involved in a wide variety of both plant pathogen-antagonistic and plant-growth-promoting activities, such as biofilm production; acetoin, butanediol, and indoleacetic acid (IAA) synthesis; production of quorum-sensing molecules; synthesis of toxins and lytic enzymes; and promotion of tolerance to oxidative, metal, and salt stress. Additionally, antiSMASH analysis revealed a potential synthesis of siderophores and peptide antibiotics. To confirm the in silico data, strain CR71 was inoculated into cucumber plants (Cucumis sativus L.) in a field trial, in which we observed an increase in stem thickness, as well as shoot fresh weight and length. Importantly, compared to un-inoculated control plants, plants inoculated with strain CR71 increased the size/weight ratio of cucumber fruits (34.99%), biovolume index (16.8%), and total fruit yield (34.97%). In conclusion, genome analysis of strain CR71 confirmed multifactorial plant-beneficial mechanisms and the potential of CR71 as an agricultural bio-inoculant.

Authors+Show Affiliations

1Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Ciudad Universitaria, 58030 Morelia, Michoacán México.2Facultad de Agrobiología "Presidente Juárez", Universidad Michoacana de San Nicolás de Hidalgo (UMSNH), Paseo Lázaro Cárdenas S/N Esq. Berlín, Col. Viveros, 60190 Uruapan, Michoacán México.2Facultad de Agrobiología "Presidente Juárez", Universidad Michoacana de San Nicolás de Hidalgo (UMSNH), Paseo Lázaro Cárdenas S/N Esq. Berlín, Col. Viveros, 60190 Uruapan, Michoacán México.2Facultad de Agrobiología "Presidente Juárez", Universidad Michoacana de San Nicolás de Hidalgo (UMSNH), Paseo Lázaro Cárdenas S/N Esq. Berlín, Col. Viveros, 60190 Uruapan, Michoacán México.3Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, Col. Centro, 85000 Ciudad Obregón, Sonora México.1Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Ciudad Universitaria, 58030 Morelia, Michoacán México.1Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Ciudad Universitaria, 58030 Morelia, Michoacán México.

Pub Type(s)

Case Reports

Language

eng

PubMed ID

32355594

Citation

Flores, Aurora, et al. "Bridging Genomics and Field Research: Draft Genome Sequence of Bacillus Thuringiensis CR71, an Endophytic Bacterium That Promotes Plant Growth and Fruit Yield in Cucumis Sativus L." 3 Biotech, vol. 10, no. 5, 2020, p. 220.
Flores A, Diaz-Zamora JT, Orozco-Mosqueda MDC, et al. Bridging genomics and field research: draft genome sequence of Bacillus thuringiensis CR71, an endophytic bacterium that promotes plant growth and fruit yield in Cucumis sativus L. 3 Biotech. 2020;10(5):220.
Flores, A., Diaz-Zamora, J. T., Orozco-Mosqueda, M. D. C., Chávez, A., de Los Santos-Villalobos, S., Valencia-Cantero, E., & Santoyo, G. (2020). Bridging genomics and field research: draft genome sequence of Bacillus thuringiensis CR71, an endophytic bacterium that promotes plant growth and fruit yield in Cucumis sativus L. 3 Biotech, 10(5), 220. https://doi.org/10.1007/s13205-020-02209-1
Flores A, et al. Bridging Genomics and Field Research: Draft Genome Sequence of Bacillus Thuringiensis CR71, an Endophytic Bacterium That Promotes Plant Growth and Fruit Yield in Cucumis Sativus L. 3 Biotech. 2020;10(5):220. PubMed PMID: 32355594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bridging genomics and field research: draft genome sequence of Bacillus thuringiensis CR71, an endophytic bacterium that promotes plant growth and fruit yield in Cucumis sativus L. AU - Flores,Aurora, AU - Diaz-Zamora,J Trinidad, AU - Orozco-Mosqueda,Ma Del Carmen, AU - Chávez,Ana, AU - de Los Santos-Villalobos,Sergio, AU - Valencia-Cantero,Eduardo, AU - Santoyo,Gustavo, Y1 - 2020/04/28/ PY - 2020/02/06/received PY - 2020/04/15/accepted PY - 2021/05/01/pmc-release PY - 2020/5/2/entrez PY - 2020/5/2/pubmed PY - 2020/5/2/medline KW - Bacterial endophyte KW - Bioinoculant KW - Field trial KW - Plant growth-promoting bacteria SP - 220 EP - 220 JF - 3 Biotech JO - 3 Biotech VL - 10 IS - 5 N2 - Here we report the draft genome sequence of bacterial strain CR71, consisting of a single chromosome with 5,914,775 base pairs (bp), 34.7% G + C content, and 5733 protein-coding genes. Phylogenetic analysis indicates that the CR71 strain is affiliated with Bacillus thuringiensis species, with an average nucleotide identity > 96% and genome to genome distance > 70%. The genome of B. thuringiensis strain CR71 contains genes potentially involved in a wide variety of both plant pathogen-antagonistic and plant-growth-promoting activities, such as biofilm production; acetoin, butanediol, and indoleacetic acid (IAA) synthesis; production of quorum-sensing molecules; synthesis of toxins and lytic enzymes; and promotion of tolerance to oxidative, metal, and salt stress. Additionally, antiSMASH analysis revealed a potential synthesis of siderophores and peptide antibiotics. To confirm the in silico data, strain CR71 was inoculated into cucumber plants (Cucumis sativus L.) in a field trial, in which we observed an increase in stem thickness, as well as shoot fresh weight and length. Importantly, compared to un-inoculated control plants, plants inoculated with strain CR71 increased the size/weight ratio of cucumber fruits (34.99%), biovolume index (16.8%), and total fruit yield (34.97%). In conclusion, genome analysis of strain CR71 confirmed multifactorial plant-beneficial mechanisms and the potential of CR71 as an agricultural bio-inoculant. SN - 2190-572X UR - https://www.unboundmedicine.com/medline/citation/32355594/Bridging_genomics_and_field_research:_draft_genome_sequence_of_Bacillus_thuringiensis_CR71,_an_endophytic_bacterium_that_promotes_plant_growth_and_fruit_yield_in_Cucumis_sativus_L L2 - https://dx.doi.org/10.1007/s13205-020-02209-1 DB - PRIME DP - Unbound Medicine ER -
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