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Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients.
Emerg Microbes Infect. 2020 Dec; 9(1):940-948.EM

Abstract

The emerging COVID-19 caused by SARS-CoV-2 infection poses severe challenges to global public health. Serum antibody testing is becoming one of the critical methods for the diagnosis of COVID-19 patients. We investigated IgM and IgG responses against SARS-CoV-2 nucleocapsid (N) and spike (S) protein after symptom onset in the intensive care unit (ICU) and non-ICU patients. 130 blood samples from 38 COVID-19 patients were collected. The levels of IgM and IgG specific to N and S protein were detected by ELISA. A series of blood samples were collected along the disease course from the same patient, including 11 ICU patients and 27 non-ICU patients for longitudinal analysis. N and S specific IgM and IgG (N-IgM, N-IgG, S-IgM, S-IgG) in non-ICU patients increased after symptom onset. N-IgM and S-IgM in some non-ICU patients reached a peak in the second week, while N-IgG and S-IgG continued to increase in the third week. The combined detection of N and S specific IgM and IgG could identify up to 75% of SARS-CoV-2 infected patients in the first week. S-IgG was significantly higher in non-ICU patients than in ICU patients in the third week. In contrast, N-IgG was significantly higher in ICU patients than in non-ICU patients. The increase of S-IgG positively correlated with the decrease of C-reactive protein (CRP) in non-ICU patients. N and S specific IgM and IgG increased gradually after symptom onset and can be used for detection of SARS-CoV-2 infection. Analysis of the dynamics of S-IgG may help to predict prognosis.

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Authors+Show Affiliations

Sun B
State Key Laboratory of Respiratory Diseases, National Clinical Research Center of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.
Feng Y
State Key Laboratory of Respiratory Diseases, National Clinical Research Center of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.
Mo X
Institute of Infectious Diseases, Guangzhou Eighth people's Hospital, Guangzhou Medical University, Guangzhou, People's Republic of China.
Zheng P
State Key Laboratory of Respiratory Diseases, National Clinical Research Center of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.
Wang Q
Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, People's Republic of China.
Li P
Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, People's Republic of China.
Peng P
Institute of Infectious Diseases, Guangzhou Eighth people's Hospital, Guangzhou Medical University, Guangzhou, People's Republic of China.
Liu X
State Key Laboratory of Respiratory Diseases, National Clinical Research Center of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.
Chen Z
Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, People's Republic of China.
Huang H
State Key Laboratory of Respiratory Diseases, National Clinical Research Center of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.
Zhang F
Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, People's Republic of China.
Luo W
State Key Laboratory of Respiratory Diseases, National Clinical Research Center of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.
Niu X
State Key Laboratory of Respiratory Diseases, National Clinical Research Center of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.
Hu P
Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, People's Republic of China.
Wang L
Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, People's Republic of China.
Peng H
Institute of Infectious Diseases, Guangzhou Eighth people's Hospital, Guangzhou Medical University, Guangzhou, People's Republic of China.
Huang Z
State Key Laboratory of Respiratory Diseases, National Clinical Research Center of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.
Feng L
Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, People's Republic of China.
Li F
Institute of Infectious Diseases, Guangzhou Eighth people's Hospital, Guangzhou Medical University, Guangzhou, People's Republic of China.
Zhang F
Institute of Infectious Diseases, Guangzhou Eighth people's Hospital, Guangzhou Medical University, Guangzhou, People's Republic of China.
Li F
Institute of Infectious Diseases, Guangzhou Eighth people's Hospital, Guangzhou Medical University, Guangzhou, People's Republic of China.
Zhong N
State Key Laboratory of Respiratory Diseases, National Clinical Research Center of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.
Chen L
State Key Laboratory of Respiratory Diseases, National Clinical Research Center of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China. Institute of Infectious Diseases, Guangzhou Eighth people's Hospital, Guangzhou Medical University, Guangzhou, People's Republic of China. Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, People's Republic of China.

MeSH

AgedAntibodies, ViralBetacoronavirusC-Reactive ProteinCOVID-19COVID-19 TestingClinical Laboratory TechniquesCoronavirus InfectionsCoronavirus Nucleocapsid ProteinsCritical CareFemaleHumansImmunoglobulin GImmunoglobulin MKineticsMaleMiddle AgedNucleocapsid ProteinsPandemicsPhosphoproteinsPneumonia, ViralSARS-CoV-2Spike Glycoprotein, Coronavirus

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32357808

Citation

Sun, Baoqing, et al. "Kinetics of SARS-CoV-2 Specific IgM and IgG Responses in COVID-19 Patients." Emerging Microbes & Infections, vol. 9, no. 1, 2020, pp. 940-948.
Sun B, Feng Y, Mo X, et al. Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients. Emerg Microbes Infect. 2020;9(1):940-948.
Sun, B., Feng, Y., Mo, X., Zheng, P., Wang, Q., Li, P., Peng, P., Liu, X., Chen, Z., Huang, H., Zhang, F., Luo, W., Niu, X., Hu, P., Wang, L., Peng, H., Huang, Z., Feng, L., Li, F., ... Chen, L. (2020). Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients. Emerging Microbes & Infections, 9(1), 940-948. https://doi.org/10.1080/22221751.2020.1762515
Sun B, et al. Kinetics of SARS-CoV-2 Specific IgM and IgG Responses in COVID-19 Patients. Emerg Microbes Infect. 2020;9(1):940-948. PubMed PMID: 32357808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients. AU - Sun,Baoqing, AU - Feng,Ying, AU - Mo,Xiaoneng, AU - Zheng,Peiyan, AU - Wang,Qian, AU - Li,Pingchao, AU - Peng,Ping, AU - Liu,Xiaoqing, AU - Chen,Zhilong, AU - Huang,Huimin, AU - Zhang,Fan, AU - Luo,Wenting, AU - Niu,Xuefeng, AU - Hu,Peiyu, AU - Wang,Longyu, AU - Peng,Hui, AU - Huang,Zhifeng, AU - Feng,Liqiang, AU - Li,Feng, AU - Zhang,Fuchun, AU - Li,Fang, AU - Zhong,Nanshan, AU - Chen,Ling, PY - 2020/5/3/pubmed PY - 2020/5/15/medline PY - 2020/5/3/entrez KW - C-reactive protein KW - COVID-19 KW - IgG KW - IgM KW - SARS-CoV-2 SP - 940 EP - 948 JF - Emerging microbes & infections JO - Emerg Microbes Infect VL - 9 IS - 1 N2 - The emerging COVID-19 caused by SARS-CoV-2 infection poses severe challenges to global public health. Serum antibody testing is becoming one of the critical methods for the diagnosis of COVID-19 patients. We investigated IgM and IgG responses against SARS-CoV-2 nucleocapsid (N) and spike (S) protein after symptom onset in the intensive care unit (ICU) and non-ICU patients. 130 blood samples from 38 COVID-19 patients were collected. The levels of IgM and IgG specific to N and S protein were detected by ELISA. A series of blood samples were collected along the disease course from the same patient, including 11 ICU patients and 27 non-ICU patients for longitudinal analysis. N and S specific IgM and IgG (N-IgM, N-IgG, S-IgM, S-IgG) in non-ICU patients increased after symptom onset. N-IgM and S-IgM in some non-ICU patients reached a peak in the second week, while N-IgG and S-IgG continued to increase in the third week. The combined detection of N and S specific IgM and IgG could identify up to 75% of SARS-CoV-2 infected patients in the first week. S-IgG was significantly higher in non-ICU patients than in ICU patients in the third week. In contrast, N-IgG was significantly higher in ICU patients than in non-ICU patients. The increase of S-IgG positively correlated with the decrease of C-reactive protein (CRP) in non-ICU patients. N and S specific IgM and IgG increased gradually after symptom onset and can be used for detection of SARS-CoV-2 infection. Analysis of the dynamics of S-IgG may help to predict prognosis. SN - 2222-1751 UR - https://www.unboundmedicine.com/medline/citation/32357808/Kinetics_of_SARS_CoV_2_specific_IgM_and_IgG_responses_in_COVID_19_patients_ DB - PRIME DP - Unbound Medicine ER -